Article
Chemistry, Medicinal
Ruiqi Huang, Vijay Gyanani, Shen Zhao, Yifan Lu, Xin Guo
Summary: In this study, three novel lipids were prepared and incorporated into PEGylated liposomes containing doxorubicin to enhance their activity against solid tumors. These lipids were designed to cluster with negatively charged phosphatidylethanolamine-polyethylene glycol at acidic tumor sites, thereby triggering the conversion of liposomes into pH-sensitive convertible liposomes. The pH drop resulted in increased positive surface charges, lipid phase separation, and aggregation with cell membrane-mimetic liposomes, leading to enhanced drug release and better anticancer activity against both monolayer cells and 3D multicellular spheroids.
Review
Pharmacology & Pharmacy
Kenan Aloss, Peter Hamar
Summary: Doxorubicin (DOX) is a potent anti-cancer agent that has attracted significant research interest for its high efficacy but dose-limiting toxicities. Various strategies, including liposomes, targeted liposomes, and pH-sensitive or thermo-sensitive liposomes combined with local heating, have been explored to enhance DOX delivery to tumors. Clinical trials have shown promising results for lyso-thermosensitive liposomal DOX (LTLD), MM-302, and C225-immunoliposomal (IL)-DOX. Additionally, preclinical models have demonstrated improved anti-tumor activity with functionalized PEGylated liposomal DOX (PLD), TSLs, and PSLs. However, further investigations are needed to address issues such as fast clearance, ligand density optimization, stability, and release rate.
Article
Chemistry, Analytical
Bing Wang, Lei Zhang, Guo Yin, Jue Wang, Ping Wang, Tiejie Wang, Jiangwei Tian, Xie-an Yu, Huachao Chen
Summary: In this study, a Argliposome-amplified colorimetric immunoassay was developed for the detection of Cys C to predict AKI. The method demonstrated rapid, highly sensitive, linear and potential for evaluating renal protective drugs.
ANALYTICA CHIMICA ACTA
(2022)
Review
Pharmacology & Pharmacy
Maryam Saadat, Farid Mostafaei, Somaye Mahdinloo, Mahdieh Abdi, Fahimeh Zahednezhad, Parvin Zakeri-Milani, Hadi Valizadeh
Summary: This review discusses the use of pH-sensitive nanoparticles for liver cancer chemotherapy, focusing on their applications in targeted delivery and gene delivery, as well as their accumulation in blood circulation and tumor media. The systems show advantages in reducing side effects and non-target cell uptake.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Physical
Liping Zhao, Mingxia Jiang, Zhilu Xu, Fengshuo Sun, Xinghan Wu, Mogen Zhang, Xiuwen Guan, Jinlong Ma, Weifen Zhang
Summary: A self-regulating photothermal conversion system based on self-assembling gold nanoparticles was designed for selective thermotherapy. The system demonstrated selective heating effect in both in vitro and in vivo models, achieving good tumor inhibition without damaging normal tissue. This work offers a novel validation protocol for tumor thermotherapy and provides an evaluation methodology for precise cancer therapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Monica C. Garcia, Jose Manuel Calderon-Montano, Manuela Rueda, Marcela Longhi, Antonio M. Rabasco, Miguel Lopez-Lazaro, Francisco Prieto-Dapena, Maria Luisa Gonzalez-Rodriguez
Summary: This study reports the development of pH/thermo-sensitive liposomes using nucleolipids and modified with gold nanoparticles. The results demonstrate that these liposomes can efficiently release anticancer drugs and exhibit significant toxicity against cancer cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Analytical
Jayoung Kim, Byeong-Seok Moon, Eunhee Hwang, Samy Shaban, Wonjung Lee, Do-Gi Pyun, Dong Hyun Lee, Dong-Hwan Kim
Summary: Researchers have developed a highly sensitive solid-state biosensor based on PDA liposomes, which can enhance sensitivity without relying on labels by adding gold nanoparticles (AuNPs) on the PDA liposomes.
Review
Pharmacology & Pharmacy
Bruna G. Carvalho, Bruno T. Ceccato, Mariano Michelon, Sang W. Han, Lucimara G. de la Torre
Summary: This article reviews the recent advances in the synthesis of lipid-based nanostructures through microfluidics, including liposomes, lipid nanoparticles, and biomimetic nanovesicles. It also discusses new strategies for controlled release of lipid payloads and the use of microfluidic systems to mimic the cellular environment. Future trends and perspectives in designing novel nano and micro scales are also discussed.
Article
Biochemistry & Molecular Biology
Anna A. Efimova, Anton S. Popov, Alexey V. Kazantsev, Pavel I. Semenyuk, Irina M. Le-Deygen, Nikolay V. Lukashev, Alexander A. Yaroslavov
Summary: pH-sensitive liposomes with embedded AMS showed rapid cargo release when pH was altered, but the mechanism is not yet fully understood. This article presents the details of fast cargo release based on ATR-FTIR spectroscopy and molecular modeling. The findings are relevant to the potential use of AMS-containing pH-sensitive liposomes for drug delivery.
Article
Biotechnology & Applied Microbiology
Lin Liu, Wendong Yao, Xiaowei Xie, Jianqing Gao, Xiaoyang Lu
Summary: A novel dual drug delivery system was designed to achieve synergistic analgesia between najanajaatra venom protein and resveratrol. The Janus nanoparticles were able to pass through intestinal epithelial cells and showed significant synergistic analgesic effects in animal models. This study provides new insights into the oral administration and analgesic treatment of dual drug delivery systems containing peptide drugs.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Hye Jin Kim, Sujin Lee, Jong Min Park, Hui Bang Cho, Ji-In Park, Ji Sun Park, Keun-Hong Park
Summary: This study developed a three-layer consecutive gene delivery system (T-CGDS) for timely gene delivery into hMSCs, promoting bone differentiation effectively. The genes were released in a time-dependent manner within the multi-layer system, demonstrating successful induction of bone regeneration both in vitro and in vivo.
Article
Nanoscience & Nanotechnology
Xiangshi Sun, Kongtong Yu, Yulin Zhou, Shiyan Dong, Wenji Hu, Yating Sun, Yuhuan Li, Jing Xie, Robert J. Lee, Fengying Sun, Yifan Ma, Shengnian Wang, Betty Y. S. Kim, Yifan Wang, Zhaogang Yang, Wen Jiang, Youxin Li, Lesheng Teng
Summary: The newly synthesized amphiphilic polymers can self-assemble into micellar nanoparticles with favorable hydrolysis kinetics, biocompatibility, and long circulation time. These materials not only exhibit better inflammation-targeting effects, but also do not induce an acidic microenvironment during degradation.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Pharmacology & Pharmacy
Aqeel Ahmad, Kirsi Rilla, Jing Zou, Weikai Zhang, Ilmari Pyykko, Paavo Kinnunen, Sanjeev Ranjan
Summary: The study demonstrates the design and characterization of an endosomolytic peptide LZEP based on the leucine zipper sequence. LZEP showed potential for membrane disruption and improved gene delivery in mammalian cells, providing a novel approach for gene therapy.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Youngdo Jeong, Soyeong Jin, L. Palanikumar, Huyeon Choi, Eunhye Shin, Eun Min Go, Changjoon Keum, Seunghwan Bang, Dongkap Kim, Seungho Lee, Minsoo Kim, Hojun Kim, Kwan Hyi Lee, Batakrishna Jana, Myoung-Hwan Park, Sang Kyu Kwak, Chaekyu Kim, Ja-Hyoung Ryu
Summary: The dynamic behavior of biological nanomachines, controlled by supramolecular recognition, has led to the development of potential therapeutic artificial nanomachines. This study integrates actuatable molecular components into a hierarchical nanomaterial to regulate biological functions in response to external stimuli, providing a strategy for designing biomimetic nanotoxins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Physical
Eunyoung Park, Donghyun Lee, Yeeun Lee, Eunjin Jeong, Sehee Kim, Heebeom Koo
Summary: The study prepared pH-sensitive liposomes for successful combination therapy with high drug accumulation in tumor tissue and simultaneous attack on nucleus and cytosol through a double hit strategy. Effective tumor suppression in a mouse model demonstrated the synergistic effect of combination therapy enabled by the pH-lipo-Dox-Pba.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Weitie Wang, Yong Wang, Hulin Piao, Bo Li, Zhicheng Zhu, Dan Li, Tiance Wang, Kexiang Liu
Summary: This study integrated miRNA and mRNA profiling analyses to demonstrate the critical roles of miR-193a-3p and ACTG2 in the pathogenesis of aortic dissection. miR-193a-3p may serve as a novel regulator of phenotypic switching in VSMCs, and the miR-193a-3p/ACTG2 axis could be a promising diagnostic biomarker and therapeutic candidate for aortic dissection.
FRONTIERS IN GENETICS
(2021)
Article
Cardiac & Cardiovascular Systems
Weitie Wang, Qing Liu, Yong Wang, Hulin Piao, Zhicheng Zhu, Dan Li, Tiance Wang, Kexiang Liu
Summary: The study reveals that linc01278 regulates VSMC phenotypic switching by controlling miR-500b-5p and ACTG2, playing a significant role in the pathogenesis of AD. This axis may have potential implications in developing diagnostic markers and therapeutic targets for AD.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Chemistry, Multidisciplinary
Weiqi Zhang, Baoji Du, Menghan Gao, Ching-Hsuan Tung
Summary: In this study, long-term lysosome tracking with minimized photodamage was achieved by using a multifunctional nanoprobe. The platinum nanoparticle scavenged light-induced reactive oxygen species, protecting lysosomal membranes from damage and enabling long-term quinacrine fluorescence imaging of lysosomes.
Article
Cell Biology
Yichen Wang, Lin Jia, Cong Wang, Zhonghua Du, Shilin Zhang, Lei Zhou, Xue Wen, Hui Li, Huiling Chen, Yuanyuan Nie, Dan Li, Shanshan Liu, Daniela Salgado Figueroa, Ferhat Ay, Wei Xu, Songling Zhang, Wei Li, Jiuwei Cui, Andrew R. Hoffman, Hui Guo, Ji-Fan Hu
Summary: Wang et al. identify Peln1 as a key chromatin long non-coding RNA that controls pluripotency exit and intrachromosomal looping in stem cells. Peln1 interacts with the Oct4 promoter, recruits DNMT3A, and alters the epigenotype to disrupt the intrachromosomal loop, leading to pluripotency exit. Peln1 also targets multiple pathway genes associated with stem cell self-renewal.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qing Dong, Xiuna Jia, Yuling Wang, Hao Wang, Qiong Liu, Dan Li, Jin Wang, Erkang Wang
Summary: Pancreatic cancer is difficult to diagnose in the early stage due to the lack of symptoms. Current diagnosis techniques have limitations in terms of personnel expertise and cost. Therefore, alternative detection methods are highly demanded. In this study, a new strategy combining nanoparticles and amplification reaction was proposed to improve the sensitivity of pancreatic cancer cell detection. The strategy showed good specificity and potential for clinical diagnosis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Xiuna Jia, Mengmeng Lv, Yunwei Fei, Qing Dong, Hao Wang, Qiong Liu, Dan Li, Jin Wang, Erkang Wang
Summary: Diagnosis-guided synergistic treatment based on innovative nanomaterials is important for the development of anti-cancer therapies. This study developed a smart carrier named LPAR, which can selectively deliver and release genes using near-infrared light and convert the light into heat for photothermal synergistic therapy. LPAR also possesses the capability of triple-modal imaging.
Article
Chemistry, Physical
Hao Wang, Jinxing Chen, Qing Dong, Xiuna Jia, Dan Li, Jin Wang, Erkang Wang
Summary: Recently, there has been increased attention on the study of mimic enzymes. However, research on the oxidoreductase activity of electron mediators in the biological respiratory chain is still limited. In this study, we found that cadmium sulfide (CdS) nanorods can catalyze the formation of superoxide anions and exhibit NADH oxidase-like activity and Cyt c reductase-like activity. This provides a new perspective for the in-depth study of new nanozymes.
Article
Chemistry, Multidisciplinary
Xiuna Jia, Deming Liu, Cong Yu, Niu Niu, Dan Li, Jin Wang, Erkang Wang
Summary: This study presents a new strategy for the rational design and development of a safe and efficient single near-infrared laser-triggered synergistic treatment platform for hypoxic cancer, based on the characteristics of the tumor microenvironment.
Article
Chemistry, Physical
Hao Wang, Jinxing Chen, Qing Dong, Xu Sun, Qiong Liu, Dan Li, Erkang Wang, Jin Wang
Summary: In this study, N, P, or S doped carbon nanotubes (CNTs) were used as enzyme mimics of nicotinamide adenine dinucleotide (NADH) oxidase and cytochrome c (Cyt c) reductase. Through N doping, the NADH oxidase-like activity of CNTs was significantly enhanced, with a 4.28-fold increase in the maximum initial velocity for N-doped CNTs (N-CNTs) compared to the unmodified ones. Biologically active NAD* was produced during NADH oxidation, and oxygen was selectively reduced to water or hydrogen peroxide, consistent with natural NADH oxidase. Additionally, it was discovered for the first time that carbon nanotubes can facilitate the electron transfer from NADH to Cyt c, mimicking the properties of Cyt c reductase.
Article
Engineering, Biomedical
Ye Zhu, Lining Zhang, Wanglin Duan, Sergio Martin-Saldana, Chaowei Li, Hongwen Yu, Luyao Feng, Xianpeng Zhang, Baoji Du, Guanying Li, Xifu Zheng, Yazhong Bu
Summary: Based on cryopolymerization, a succinic ester-based sponge (Ssponge) is fabricated and shows better hemostasis performance and tissue regeneration in liver hemorrhage and liver regeneration models compared to commercially available gelatin sponge (Csponge).
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Oncology
Huikun Niu, Dexiang Wang, Tingting Wen, Han Liu, Jing Jie, Lei Song, Dan Li
Summary: Background: Anwuligan (ANW), isolated from nutmeg, has been found to have potential therapeutic effects in diseases. However, its effect on lung cancer is still unclear. Methods: In this study, the cytotoxicity of ANW on non-small cell lung cancer (NSCLC) cells was investigated using various assays. Results: ANW treatment showed clear cytotoxicity against NSCLC cells, inhibiting cell proliferation and migration both in vitro and in vivo. The overexpression of let-7c-3p also inhibited the growth of NSCLC cells. Conclusions: ANW inhibits the growth and metastasis of NSCLC cells by upregulating the expression of let-7c-3p and targeting PIK3CA.
Article
Chemistry, Multidisciplinary
Haiwei Hou, Lan Liu, Qiuyue Li, Jianming Wang, Baoji Du
Summary: A cascade enzyme system of peroxidase and catalase is proposed in this study to expand the linear range of hydrogen peroxide (H2O2) detection. The system, consisting of ruthenium nanoparticles, catalase, and graphene, demonstrates an expanded linear range and higher maximum linear range for H2O2 detection.
Article
Engineering, Biomedical
Shuaining Ma, Weiguo Xu, Yunwei Fei, Dan Li, Xiuna Jia, Jin Wang, Erkang Wang
Summary: A TME-responsive nanoplatform is designed for synergistic photothermal therapy and photodynamic therapy against cancer. The nanoplatform allows for effective tumor accumulation and payload release through aptamer modification and acid degradable shell. It also exhibits excellent photodynamic therapy properties and achieves synergistic therapy through a single wavelength of near-infrared laser.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Physical
Hao Wang, Jinxing Chen, Qing Dong, Xu Sun, Qiong Liu, Dan Li, Erkang Wang, Jin Wang
Summary: Most nanozyme research focuses on oxidase and peroxidase capabilities, but this study explores N, P, or S doped carbon nanotubes (CNTs) as enzyme mimics of NADH oxidase and Cyt c reductase. Doping N significantly enhances the NADH oxidase-like activity of CNTs, increasing the maximum initial velocity by 4.28 times. NAD(+) is produced as a biologically active product during NADH oxidation, while oxygen is selectively reduced to water or hydrogen peroxide, similar to natural NADH oxidase. Additionally, carbon nanotubes can promote electron transfer from NADH to Cyt c, mimicking the properties of Cyt c reductase.
Article
Chemistry, Analytical
Wei Zhang, Ling Wang, Dan Li, Douglas H. Campbell, Bradley J. Walsh, Nicolle H. Packer, Qing Dong, Erkang Wang, Yuling Wang
Summary: Circulating sEVs derived from PDAC cells have potential applications in cancer diagnosis. The phenotype of sEVs is closely associated with the presence and progression of PDAC, and can be validated through various imaging techniques. The proposed SERS assay enables simultaneous detection of multiple surface membrane proteins on sEVs, providing a potential method for PDAC diagnosis and early cancer stage prediction.
ANALYTICAL METHODS
(2022)
