期刊
NATURE PROTOCOLS
卷 8, 期 11, 页码 2098-2104出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2013.138
关键词
-
资金
- Yokohama City University
- grant for Establishment of Research Center for Clinical Proteomics of Post-translational Modifications
- [20116005]
- [24390371]
- [24770216]
- Grants-in-Aid for Scientific Research [24770216] Funding Source: KAKEN
The in vitro propagation of mouse spermatogonial stem cells (SSCs) became possible in 2003; these cultured SSCs were named germ-line stem (GS) cells. To date, however, it has not been possible to induce spermatogenesis from GS cells in vitro. Recently, we succeeded in producing functional sperm from primitive spermatogonia in explanted neonatal mouse testis tissues. Here we describe a protocol that can support spermatogenesis from GS cells up to sperm formation in vitro using an organ culture method. GS cells transplanted in the extracted testis form colonies in the tissue fragments and differentiate into sperm under the described in vitro organ culture conditions. It takes about 6 weeks to obtain sperm from GS cells. The sperm are viable, resulting in healthy offspring through micro-insemination. Thus, this protocol should be a valuable tool for the study of mammalian spermatogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据