Article
Multidisciplinary Sciences
Shelly Reuven, Mieka Rinsky, Vera Brekhman, Assaf Malik, Oren Levy, Tamar Lotan
Summary: In cnidarians like Nematostella vectensis, long-term ecological success relies on sexual reproduction, with temperature and light being major inducers for spawning. Transcriptome analysis revealed upregulation of various processes like receptors and cytoskeleton during spawning induction, while downregulation of cell cycle and metabolism-related processes was observed. Our findings also suggest temperature change as a stronger inducer for spawning in Nematostella, expanding our understanding of sexual reproduction in cnidarians.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Laura Baldassarre, Hua Ying, Adam Reitzel, Soren Franzenburgq, Sebastian Fraune
Summary: This study shows that sea anemones acclimated to high temperatures demonstrate increased resistance to thermal stress and that this improved adaptation can be transferred through microbiome transplantation. The findings suggest that microbiome plasticity may play a crucial role in thermal adaptations in animals.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Layla Al-Shaer, Whitney Leach, Noor Baban, Mia Yagodich, Mathew C. C. Gibson, Michael J. J. Layden
Summary: We investigate factors influencing asexual reproduction in Nematostella vectensis, a burrowing sea anemone capable of transverse fission. Our study demonstrates that the presence of a burrowing substrate strongly promotes transverse fission and animal size does not affect fission rates. Gene expression analysis suggests that transcription factors and signaling pathways play vital roles in regulating transverse fission. Additionally, we find that the cell cycle is suppressed and cell adhesion and patterning mechanisms are downregulated during fission. This research provides insights into the reproductive biology of cnidarians.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Biochemical Research Methods
Casimir Bamberger, Sandra Pankow, John R. Yates
Summary: The study finds that nvp63 may play a role in controlling genome stability in response to DNA damage, particularly those induced by the activation of transposable elements.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Microbiology
Anthony M. Bonacolta, Michael T. Connelly, Stephanie M. Rosales, Javier del Campo, Nikki Traylor-Knowles
Summary: Sampling of different body regions can reveal highly specialized bacterial associations within the holobiont and facilitate identification of core microbial symbionts that would otherwise be overlooked by bulk sampling methods. Through compartment-specific sampling of the model cnidarian Nematostella vectensis, a dominance of spirochetes in the capitulum was uncovered, with a phylogenetic analysis showing a close relation to spirochetes previously recovered from wild N. vectensis, suggesting a prevalent and yet uncharacterized association between N. vectensis and spirochetes from the order Spirochaetales.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Laura Baldassarre, Adam M. Reitzel, Sebastian Fraune
Summary: Most multicellular organisms have microbial colonizers that provide benefits to the hosts. This study examines the relative contribution of environment and host genotype to bacterial community composition in Nematostella vectensis, a marine organism. The results show that bacterial communities in N. vectensis respond to changes in ambient temperature and are influenced by host genotype. This study highlights the importance of understanding the plasticity of microbial communities in response to environmental changes.
Article
Multidisciplinary Sciences
Leslie S. Babonis, Camille Enjolras, Joseph F. Ryan, Mark Q. Martindale
Summary: This study reveals the developmental mechanism of a truly novel cell type, cnidocytes. Cnidocytes develop by suppressing the neural fate of a subset of neurons expressing RFamide. A single regulatory gene, a C2H2-type zinc finger transcription factor (ZNF845), plays a crucial role in the development of cnidocytes and emerged through domain shuffling in the ancestor of cnidarians.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Oceane Tourniere, James M. Gahan, Henriette Busengdal, Natascha Bartsch, Fabian Rentzsch
Summary: Using the sea anemone Nematostella vectensis as a model system, researchers found that neurons and secretory cells derive from a common pool of progenitor cells, with NvInsm1 playing a crucial role in this process, revealing a close evolutionary relationship between neurons and secretory cells.
Article
Multidisciplinary Sciences
Quentin I. B. Lemaitre, Natascha Bartsch, Ian U. Kouzel, Henriette Busengdal, Gemma Sian Richards, Patrick R. H. Steinmetz, Fabian Rentzsch
Summary: Endodermal neurogenesis is a rare phenomenon in animals. Researchers have identified a group of endoderm-specific neural progenitor cells in the cnidarian Nematostella vectensis, characterized by the expression of the transcription factor prdm14d. This study sheds light on the molecular regulation of non-ectodermal neurogenesis using Nematostella vectensis as a model, providing new insights into this poorly understood process.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Hanny E. Rivera, Cheng-Yi Chen, Matthew C. Gibson, Ann M. Tarrant
Summary: Parental exposure to elevated temperatures can increase larval thermal tolerance in Nematostella vectensis, with subsequent spawns returning to baseline thresholds when parents are back to normal temperatures, indicating plasticity in parental effects. Maternal effects in gametogenesis may play a role in facilitating dynamic shifts in larval thermal tolerance. Despite genetic differences, parental populations from different locations can increase larval thermal tolerance under high-temperature conditions, suggesting that plasticity in parental effects is inherent in N. vectensis.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Cell Biology
Arie Fridrich, Miguel Salinas-Saaverda, Itamar Kozlolvski, Joachim M. Surm, Eleni Chrysostomou, Abhinandan M. Tripathi, Uri Frank, Yehu Moran
Summary: The study reveals the importance of miR-2022 in initiating cell generation in Nematostella and its conservation across other cnidarian species.
Article
Multidisciplinary Sciences
Alison G. G. Cole, Stefan M. M. Jahnel, Sabrina Kaul, Julia Steger, Julia Hagauer, Andreas Denner, Patricio Ferrer Murguia, Elisabeth Taudes, Bob Zimmermann, Robert Reischl, Patrick R. H. Steinmetz, Ulrich Technau
Summary: Gene duplications of bHLH transcription factors and effector genes contribute to the diversification of muscle cell types in the sea anemone Nematostella.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
James M. Gahan, Ian U. Kouzel, Kamilla Ormevik Jansen, Pawel Burkhardt, Fabian Rentzsch
Summary: This study reveals the role of chromatin modifier Lsd1 in developmental regulation in cnidarians. The findings suggest that the integration of chromatin modifying proteins into developmental regulation predates the divergence of cnidarians and bilaterians, and is an ancient feature of animal development.
NATURE COMMUNICATIONS
(2022)
Article
Ecology
Shani Levy, Vera Brekhman, Anna Bakhman, Assaf Malik, Arnau Sebe-Pedros, Mickey Kosloff, Tamar Lotan
Summary: The study demonstrates that ectopic activation of GABA(B)R signaling affects neurogenic functions in the sea anemone Nematostella vectensis. The sustained activation of GABA(B)R signaling reversibly arrests the critical metamorphosis transition from planktonic larva to sessile polyp life stage. The findings point to an evolutionarily conserved function of GABA(B)R in neurogenesis regulation and shed light on early cnidarian development.
NATURE ECOLOGY & EVOLUTION
(2021)
Article
Biology
Wiebke Ehrlich, James M. Gahan, Fabian Rentzsch, Frank J. P. Kuehn
Summary: This study investigates the physiological function of TRPM2 in N. vectensis and demonstrates that it sensitizes to oxidative stress while attenuates high-temperature injury in this marine invertebrate.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Longjun Wu, Laurel S. Hiebert, Marleen Klann, Yale Passamaneck, Benjamin R. Bastin, Stephan Q. Schneider, Mark Q. Martindale, Elaine C. Seaver, Svetlana A. Maslakova, J. David Lambert
NATURE COMMUNICATIONS
(2020)
Article
Environmental Sciences
Catherine B. Eastman, Jessica A. Farrell, Liam Whitmore, Devon R. Rollinson Ramia, Rachel S. Thomas, Jenifer Prine, Scott F. Eastman, Todd Z. Osborne, Mark Q. Martindale, David J. Duffy
FRONTIERS IN MARINE SCIENCE
(2020)
Article
Zoology
Dorothy G. Mitchell, Allison Edgar, Mark Q. Martindale
Summary: The study found that a household water repellant glass treatment product can significantly improve the morphological preservation of gelatinous zooplankton, facilitating long-term storage and detection of target molecules for further research. Extending this method may enhance the morphological fidelity and durability of museum and laboratory specimens for other delicate sample types.
FRONTIERS IN ZOOLOGY
(2021)
Review
Genetics & Heredity
Allison Edgar, Dorothy G. Mitchell, Mark Q. Martindale
Summary: Ctenophores, including Mnemiopsis leidyi, exhibit varied regenerative abilities within the group. M. leidyi is known for its rapid wound healing, tissue replacement, and stereotyped development, making it a popular model for research. While some cell signaling pathways related to regeneration in other animals are absent in ctenophores, further research may provide insights into ancient regenerative principles or novel solutions for cell fate stability and whole-body regeneration.
Article
Biochemistry & Molecular Biology
Robert E. Jinkerson, Joseph A. Russo, Casandra R. Newkirk, Andrea L. Kirk, Richard J. Chi, Mark Q. Martindale, Arthur R. Grossman, Masayuki Hatta, Tingting Xiang
Summary: Photosynthesis is not necessary for symbiosis establishment, but its impact depends on specific cnidarian-Symbiodiniaceae relationships. UV mutagenesis can generate photosynthetic mutants capable of infecting hosts in the absence of photosynthesis.
Article
Multidisciplinary Sciences
Allison Edgar, Jose Miguel Ponciano, Mark Q. Martindale
Summary: Research shows that ctenophores, specifically Mnemiopsis leidyi, do not have separate phases of early and adult reproduction. They start reproducing at a small body size and continue spawning under suitable environmental conditions. The constraints on reproductive capacity are similar in both small and large animals. These findings have significant implications for understanding resource partitioning and managing the species in its invaded range.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Leslie S. Babonis, Camille Enjolras, Joseph F. Ryan, Mark Q. Martindale
Summary: This study reveals the developmental mechanism of a truly novel cell type, cnidocytes. Cnidocytes develop by suppressing the neural fate of a subset of neurons expressing RFamide. A single regulatory gene, a C2H2-type zinc finger transcription factor (ZNF845), plays a crucial role in the development of cnidocytes and emerged through domain shuffling in the ancestor of cnidarians.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Evolutionary Biology
Bailey M. Steinworth, Mark Q. Martindale, Joseph F. Ryan
Summary: Hox and ParaHox transcription factors play crucial roles in determining cell fates in animal development. In this study, researchers analyzed the complete set of Hox and ParaHox genes from various species of cnidarians, including all four medusozoan classes and both anthozoan classes. The results showed that the Hox genes involved in patterning anthozoan polyps are missing in the stem leading to Medusozoa. Additionally, the study provided new insights into the spatial and temporal expression patterns of Hox and ParaHox genes in a Scyphozoa jellyfish.
GENOME BIOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Jennifer Ortiz, Yuriy Bobkov, Melissa B. DeBiasse, Dorothy G. Mitchell, Allison Edgar, Mark Q. Martindale, Anthony G. Moss, Leslie S. Babonis, Joseph F. Ryan
Summary: This article investigates the innexin gene in four species of ctenophores and finds that these genes have evolved independently from those of other animals and were established early in the evolution of ctenophores. The study also shows that ctenophores have functional innexin hemichannels that are closely related to those of other animals. Overall, the results suggest that the last common ancestor of animals had channels capable of forming both gap junctions and innexin hemichannels, and that innexin genes have independently evolved in major lineages throughout Metazoa.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Letter
Multidisciplinary Sciences
Allison Edgar, Jose Miguel Ponciano, Mark Q. Martindale
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Labib Rouhana, Allison Edgar, Fredrik Hugosson, Valeria Dountcheva, Mark Q. Martindale, Joseph F. Ryan
Summary: Differential regulation of gene expression is crucial for the astonishing diversity of life on Earth. Cytoplasmic polyadenylation, mediated by the CPEB family, regulates the translation of maternal transcripts and is present in animals but not non-animal lineages. Phylogenetic analyses reveal that CPEB1 and CPEB2 originated in the animal stem lineage. This study demonstrates the conservation of maternal expression and polyadenylation targets in cnidarians, ctenophores, and vertebrates, suggesting that cytoplasmic polyadenylation through CPEBs was a fundamental innovation in animal evolution.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Leslie S. Babonis, Camille Enjolras, Abigail J. Reft, Brent M. Foster, Fredrik Hugosson, Joseph F. Ryan, Marymegan Daly, Mark Q. Martindale
Summary: Cnidocytes are explosive stinging cells unique to cnidarians, such as corals and jellyfish. Using CRISPR/Cas9-mediated genome editing, researchers have discovered that a single transcription factor, NvSox2, is responsible for determining two alternative fates of stinging cells in the sea anemone Nematostella vectensis. Knockout of NvSox2 transforms piercing cells into ensnaring cells, providing insights into the diversification of cell types.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Pei Zhang, Yuanzhen Zhu, Qunfei Guo, Ji Li, Xiaoyu Zhan, Hao Yu, Nianxia Xie, Huishuang Tan, Nina Lundholm, Lydia Garcia-Cuetos, Michael D. Martin, Meritxell Anto Subirats, Yi-Hsien Su, Inaki Ruiz-Trillo, Mark Q. Martindale, Jr-Kai Yu, M. Thomas P. Gilbert, Guojie Zhang, Qiye Li
Summary: By analyzing the RNA editomes of 22 Holozoa species, we provide substantial evidence that A-to-I mRNA editing is a regulatory innovation that originated in the last common ancestor of extant metazoans. This ancient biochemical process is preserved in most metazoans and primarily targets endogenous dsRNA formed by young repeats. We also find that intermolecular pairing of sense-antisense transcripts is an important mechanism for forming dsRNA substrates for A-to-I editing in some lineages. Recoding editing is rarely shared across lineages but preferentially targets genes involved in neural and cytoskeleton systems in bilaterians. We conclude that metazoan A-to-I editing initially emerged as a safeguard mechanism against repeat-derived dsRNA and was later co-opted into diverse biological processes due to its mutagenic nature.
Article
Chemistry, Physical
S. Tori Ellison, Senthilkumar Duraivel, Vignesh Subramaniam, Fredrik Hugosson, Bo Yu, Joseph J. Lebowitz, Habibeh Khoshbouei, Tanmay P. Lele, Mark Q. Martindale, Thomas E. Angelini
Summary: Cell type variation plays a crucial role in tissue physiology. To better understand the relationship between tissue structure and function, and to engineer accurate tissues, it is necessary to assemble 3D cellular structures with detailed precision. In this study, a method of 3D cell assembly with subcellular precision is introduced. The authors demonstrate the ability to create detailed cellular patterns and show functional characteristics after assembly.
Article
Engineering, Multidisciplinary
Jeremy Elias, Thomas Angelini, Mark Q. Martindale, Laurie Gower
Summary: Cellular strategies and regulation are crucial for the formation of biominerals. By studying the interaction between organic matrices and mineral precursors under different temperatures and seawater compositions, we identified suitable conditions for calcium carbonate infiltration. These findings are valuable for the development of novel biosynthetic materials.