Article
Chemistry, Multidisciplinary
Yazan Bdour, Graham Beaton, Juan Gomez-Cruz, Oscar Cabezuelo, Kevin Stamplecoskie, Carlos Escobedo
Summary: A surface-enhanced Raman scattering (SERS) active metasurface composed of metallic nanohole arrays and metallic nanoparticles has been developed. The metasurface can operate in aqueous environments, achieving an enhancement factor of 1.83 x 10(9) for Rhodamine 6G, and enabling the detection of malachite green at a concentration of 0.46 ppb.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yi Hou, Shuai Yuan, Guangda Zhu, Baihao You, Ying Xu, Wenxin Jiang, Ho Cheung Shum, Philip W. T. Pong, Chia-Hung Chen, Liqiu Wang
Summary: In this study, a 3D composite photonic crystal (PC)-based optoelectronic device is designed for fast, naked-eye, high-resolution ultratrace detection. The mechanically strong ellipsoidal composite PCs are hand-machined into rectangular geometries, allowing them to be easily integrated with the flat light-detection area. The developed PC-based device shows improved signal-to-noise ratio, enabling rapid identification of ultratrace analytes and detection of glucose in artificial sweat.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Raj Kishen Radha Krishnan, Shiyi Liu, Drona Dahal, Pushpa Raj Paudel, Bjorn Lussem
Summary: The mechanism of doping at ultralow doping concentrations was studied, resulting in the successful realization of doped C-60 OFETs with ultralow operation voltage and high on/off ratio.
ADVANCED OPTICAL MATERIALS
(2021)
Article
Chemistry, Analytical
Serge G. Lemay, Taghi Moazzenzade
Summary: This article discusses the challenges of quantifying ultralow analyte concentrations in electrochemistry and introduces the method of digital sensing to address these challenges, using large arrays of separately addressable single-entity detectors to provide real-time information on individual binding events.
ANALYTICAL CHEMISTRY
(2021)
Article
Optics
Reza Hadjiaghaie Vafaie, Hamid Heidarzadeh
Summary: The research focused on improving mass transport limitations in SPR-based biosensors by coupling them with a microfluidic system and employing an AC electrothermal concentrator. Multi-physical modeling using finite element method was conducted to study the enhanced plasmonic biosensor. Numerical results showed that the proposed method enhances the analyte-receptor binding reaction and reduces the response time effectively.
OPTICS AND LASER TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Neda Hosseinpour Moghadam, Rezvan Najafi, Ali Ghanbariasad, Amin Shiralizadeh Dezfuli, Akram Jalali
Summary: The study develops an optical biosensor for COVID-19 detection using the localized surface plasmon resonance (LSPR) phenomenon. Two types of plasmonic nanoparticles, AuNPs and hexagonal core-shell nanoparticles (Ag@AuNPs), are synthesized. The biosensors employ efficient targeting and binding abilities for three regions of the COVID-19 genome and achieve high sensitivity and specificity. This selective, reliable, reproducible, and visual 'naked-eye' detection method provides an alternative to sophisticated instrumental techniques.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Analytical
Karthikeyan Kandasamy, Miftakhul Jannatin, Yu-Chie Chen
Summary: A rapid detection method for bacteria on sample surfaces was developed based on the endogenous enzymatic reactions of E. coli and S. aureus. The presence of the bacteria can be visually confirmed through the appearance of blue color in the swab and the formation of air bubbles in the reagent solution after two-step enzymatic reactions.
Article
Biochemical Research Methods
Xuejun Wang, Changhao Dai, Yungeng Wu, Yunqi Liu, Dacheng Wei
Summary: This study presents a testing platform using a molecular-electromechanical system (MolEMS) immobilized on graphene field-effect transistors for the analysis of trace analytes in biofluids. MolEMS achieves unamplified detection of proteins, ions, small molecules, and nucleic acids within minutes and has wide-ranging applications. This article provides step-by-step procedures for MolEMS design and assemblage, sensor manufacture, and operation in various applications.
Article
Chemistry, Multidisciplinary
Mingxi Wu, Guohua Li, Xiangyi Ye, Bin Zhou, Jianhua Zhou, Jingxuan Cai
Summary: This study presents the design and fabrication of a novel ultrasensitive plasmonic metasurface sensor for label-free and spectrometer-free detection of trace molecules. By integrating it with a microfluidic setup, quantitative characterization of biomolecular interactions is achieved with a low manufacturing cost.
Article
Chemistry, Multidisciplinary
Zhimin Song, Yan Liu, Yongzhi Zhong, Qing Guo, Jie Zeng, Zhigang Geng
Summary: A Cu-based catalyst, Cu@C, is developed for the electroreduction of nitrate at ultralow concentrations, achieving high Faradaic efficiency and NH3 yield rate. The mechanism involves concentration of NO3- enabled by the porous carbon framework, facilitating efficient electroreduction into NH3.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Analytical
Meihui Cui, Huaqing Yang, Binglin Ma, Shenjunjie Lu, Dawei Sun, Minye Yang, Kang Peng, Shufang Zhang, Jing Liu, Peiyuan Liu, Hanjie Wang
Summary: This study presents a portable platform for naked-eye detection of OTA using photonic crystal inverse opal hydrogel films based on smartphones. The platform allows for rapid and accurate detection of OTA concentration, with exceptional reliability and convenience. Additionally, it is versatile for detecting other mycotoxins.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Paula M. Castillo, Francisco J. Fernandez-Acejo, Jose M. Carnerero, Rafael Prado-Gotor, Aila Jimenez-Ruiz
Summary: The study utilizes the stabilizing effect of lysozymes to develop a quick naked-eye test for detecting urinary lysozyme concentrations over 57.2 μg/mL. By calculating color difference parameters, the test can reliably provide a positive colorimetric response in less than 15 minutes, making it useful for detecting disorders such as leukemia-associated lysozymurias.
Article
Nanoscience & Nanotechnology
Gaurav Sinsinbar, Sushanth Gudlur, Bo Liedberg
Summary: Recent advancements in microbial contamination detection methods have facilitated the development of on-site and point-of-care testing for food and water samples. This study presents a novel fluorescence-based OmptinPolythiophene assay for the rapid and reliable detection of Escherichia coli in contaminated water samples. The assay demonstrates a significant reduction in detection time, from 1 hour to just 10 minutes, and includes colorimetric sensing capability for easy visual interpretation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Food Science & Technology
Alejandro Garrido-Maestu, Marta Prado
Summary: Nucleic acid amplification-based techniques, particularly isothermal amplification approaches, have gained acceptance and interest due to their ability to overcome the need for specialized equipment and their potential for combined naked-eye detection strategies. This review provides up-to-date information on different types of naked-eye detection strategies combined with isothermal amplification, allowing readers to select the most appropriate strategies based on their specific needs and resources.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Chemistry, Multidisciplinary
Yang Xiong, Tong Fu, Daxiao Zhang, Shunping Zhang, Hongxing Xu
Summary: The research presents a biosensor based on superradiative plasmonic nanoantennas for simple and sensitive detection of biomarkers. By enhancing light scattering through strong coupling between gold nanoparticles and a gold mirror, the method allows observation of captured Au NPs with the naked eye. This technique offers a broad dynamic range, low detection limit, and precise quantification of biomarkers.
Article
Chemistry, Multidisciplinary
Rujie Sun, Xin Song, Kun Zhou, Yuyang Zuo, Richard Wang, Omar Rifaie-Graham, David J. Peeler, Ruoxiao Xie, Yixuan Leng, Hongya Geng, Giulia Brachi, Yun Ma, Yutong Liu, Lorna Barron, Molly M. Stevens
Summary: This study presents a versatile platform for fabricating fillable microrobots using microfluidic loading and dip sealing (MLDS), which allows for encapsulating different types of cargoes within hollow microrobots and protecting the cargo integrity. The technique is supported by high-resolution 3D printing and an integrated microfluidic loading system, enabling a highly precise loading process and improved cargo loading capacity. The MLDS platform offers new opportunities for targeted drug delivery, environmental sensing, and chemically powered micromotor applications.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Omar Rifaie-Graham, Jonathan Yeow, Adrian Najer, Richard Wang, Rujie Sun, Kun Zhou, Tristan N. Dell, Christopher Adrianus, Chalaisorn Thanapongpibul, Mohamed Chami, Stephen Mann, Javier Read de Alaniz, Molly M. Stevens
Summary: This study describes a non-equilibrium nanosystem that utilizes light-gated chemical communication, controllable feedback, and macroscopic oscillations. The system allows for communication between nanoreactors and induces chemical mechanical oscillations in a hydrogel using feedback loops.
Article
Biotechnology & Applied Microbiology
Taras Sych, Jan Schlegel, Hanna M. G. Barriga, Miina Ojansivu, Leo Hanke, Florian Weber, R. Beklem Bostancioglu, Kariem Ezzat, Herbert Stangl, Birgit Plochberger, Jurga Laurencikiene, Samir El Andaloussi, Daniel Fuerth, Molly M. Stevens, Erdinc Sezgin
Summary: Lipid nanoparticles, viruses, exosomes and liposomes can be characterized using fluorescence fluctuations analysis. A method called single-particle profiler is introduced to provide information on the content and biophysical properties of thousands of particles within the size range of 5-200 nm. The single-particle profiler is utilized to measure mRNA encapsulation efficiency of lipid nanoparticles, viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
NATURE BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Andreu Vaquer, Cristina Adrover-Jaume, Antonio Clemente, Amanda Iglesias, Meritxell Lopez, Rocio Martinez, Isabel M. Roig, Borja G. Cosio, Roberto de la Rica
Summary: A new method for infusing porous materials with concentrated polymers is proposed, which solves the issue of high viscosity during conventional printing methods and reduces manufacturing variability of biosensors. Immunosenors for detecting cytokines IL-6 and IL-5 were developed using this method and showed promising results.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Sebastien J. P. Callens, Daniel Fan, Ingmar A. J. van Hengel, Michelle Minneboo, Pedro J. Diaz-Payno, Molly M. Stevens, Lidy E. Fratila-Apachitei, Amir A. Zadpoor
Summary: The organization of bone cells in landscapes with varying curvatures was studied, revealing a preference for local concavities, multicellular bridging, and collective stress fiber orientation.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Hongya Geng, Emily J. Lupton, Yun Ma, Rujie Sun, Christopher L. Grigsby, Giulia Brachi, Xiaorui Li, Kun Zhou, Daniel J. Stuckey, Molly M. Stevens
Summary: The development of near-infrared light responsive conductive polymers provides a useful theranostic platform for malignant tumors. The self-assembly of ultrathin 2D polypyrrole nanosheets with tunable size distribution enables photoacoustic imaging and photothermal therapy. The pH-responsive features of the nanosheets show potential for accurate and highly efficient theranostics.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Anu K. Solanki, Helene Autefage, Antonio R. Rodriguez, Shweta Agarwal, Joaquin Penide, Muzamir Mahat, Thomas Whittaker, Amy Nommeots-Nomm, Elena Littmann, David J. Payne, Anthony D. Metcalfe, Felix Quintero, Juan Pou, Molly M. Stevens, Julian R. Jones
Summary: In this study, novel glass fibers were developed to improve chronic wound healing by activating the HIF pathway. The results showed that the glass fibers released therapeutic ions, including cobalt, which greatly promoted the expression of angiogenic genes. This suggests the potential use of these fibers in chronic wound dressings.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Catherine Saunders, Camille A. de Villiers, Molly M. Stevens
Summary: Nanoparticles have great potential in medicine, but their manufacturing and quality control present challenges to their clinical translation. Advancements in single particle techniques can help overcome these challenges and facilitate the development of future nanoformulations.
Article
Chemistry, Multidisciplinary
Kaja I. Ritzau-Reid, Sebastien J. P. Callens, Ruoxiao Xie, Martina Cihova, Daniel Reumann, Christopher L. Grigsby, Lino Prados-Martin, Richard Wang, Axel C. Moore, James P. K. Armstrong, Juergen A. Knoblich, Molly M. Stevens
Summary: 3D organoids are widely used in vitro models for studying human development and disease. However, the current culture methods have limitations in terms of reproducibility and geometric control. A new approach combining stem cell biology and bioengineering is presented, using melt electrospinning writing to generate grid scaffolds that guide the self-organization of pluripotent stem cells. This method allows for high-throughput generation, culture, and analysis of organoids, reducing the time and labor involved in traditional methods.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Junfang Zhang, Yuntao Zhu, Christian Njel, Yuxin Liu, Pietro Dallabernardina, Molly M. Stevens, Peter H. Seeberger, Oleksandr Savateev, Felix F. Loeffler
Summary: In this study, a new synthesis strategy for carbon nitride photoelectrodes was introduced through advanced film generation techniques. This method involved spin coating pretreatment of a conductive substrate with a multipurpose polymer and a supramolecular precursor, followed by chemical vapor deposition to synthesize dual-layer carbon nitride photoelectrodes. The polymer improved the polymerization degree and electrical conductivity of carbon nitride, leading to state-of-the-art photoelectrochemical performance and high yields in C-H functionalization.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Manuel Gutierrez-Capitan, Ana Sanchis, Estela O. Carvalho, Antonio Baldi, Lluisa Vilaplana, Vanessa F. Cardoso, Alvaro Calleja, Mingxing Wei, Roberto de la Rica, Javier Hoyo, Arnau Bassegoda, Tzanko Tzanov, Maria-Pilar Marco, Senentxu Lanceros-Mendez, Cesar Fernandez-Sanchez
Summary: This study demonstrates a unique and easily deployable multiplex device that can simultaneously measure interleukin-8, tumor necrosis factor-α, and myeloperoxidase biomarkers in sputum, aiming to facilitate the timely detection of acute exacerbations of chronic obstructive pulmonary disease.
Article
Multidisciplinary Sciences
Daniel W. Hagey, Miina Ojansivu, Beklem R. Bostancioglu, Osama Saher, Jeremy P. Bost, Manuela O. Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M. Stevens, Andre Goergens, Samir EL Andaloussi
Summary: This study provides important insights into the cellular response to extracellular vesicles (EVs), showing that EV dose has a more significant effect than cell source. At high doses, exocytosis is down-regulated and lysosomal activity is up-regulated. However, at low doses, specific responses based on EV cell source are observed, reflecting the activities of the EV's source cells. Furthermore, immune cell-derived EVs are most associated with recipient cells.
Article
Chemistry, Analytical
David Delgado-Cano, Antonio Clemente, Cristina Adrover-Jaume, Andreu Vaquer, Meritxell Lopez, Rocio Martinez, Isabel M. Roig, Amanda Iglesias, Borja G. Cosio, Roberto de la Rica
Summary: Rapid detection and treatment of Pseudomonas aeruginosa (P. aeruginosa) infections in the respiratory tract of patients with chronic lung diseases is crucial. In this study, an alternative diagnosis approach based on analyzing breath aerosols was proposed. The use of nanoparticle immunosensors on the polypropylene layer of surgical facemasks worn by patients allowed for the detection of chronic and acute P. aeruginosa lung infections.
Article
Biochemical Research Methods
Vernon LaLone, Aleksandra Aizenshtadt, John Goertz, Froydis Sved Skottvoll, Marco Barbero Mota, Junji You, Xiaoyu Zhao, Henriette Engen Berg, Justyna Stokowiec, Minzhi Yu, Anna Schwendeman, Hanne Scholz, Steven Ray Wilson, Stefan Krauss, Molly M. Stevens
Summary: Confocal Raman spectral imaging (RSI) enables label-free visualization of molecules in biological specimens without sample preparation. qRamanomics, an integrated bioanalytical methodology, is developed to quantify the deconvoluted spectra for quantitative spatial chemotyping of biomolecules. It is applied to assess specimen variation and maturity in 3D liver organoids and identify biomolecular response signatures from liver-altering drugs, facilitating drug metabolism and accumulation monitoring.
CELL REPORTS METHODS
(2023)
Article
Chemistry, Multidisciplinary
Jules Lee, Prajakatta Mulay, Matthew J. Tamasi, Jonathan Yeow, Molly M. Stevens, Adam J. Gormley
Summary: Oxygen tolerant polymerizations allow for high-throughput synthesis of diverse polymer architectures. The development of a robotic platform enables fully automated PET-RAFT polymerizations with individual control of reactions. This platform successfully synthesizes various polymers with high conversions and low dispersity. With the inclusion of machine learning protocols, this robotic platform has the potential to become a self-driving lab for dispensing, synthesizing, and monitoring large polymer libraries.
