Article
Cell Biology
Lu Xiao, Juan Zhu, Zheng Liu, Bangyong Wu, Xiaohua Zhou, Yanxing Wei, Fei Sun, Zhijian Wang, Song Quan, Qi Li, Jun Wang, Liping Huang, Yanlin Ma
Summary: This study discovered that human embryonic stem cells grown in different environments exhibited different gene expression patterns, with significant differential expression of 23 lncRNAs and 15 genes in different culture conditions. Gene ontology analysis showed that these genes were involved in processes such as growth factor stimuli, cell growth, and stem cell maintenance.
Article
Veterinary Sciences
Nakami Wilkister Nabulindo, James Nguhiu-Mwangi, Ambrose Ng'eno Kipyegon, Moses Ogugo, Charity Muteti, Tiambo Christian, Melissa J. Oatley, Jon M. Oatley, Stephen Kemp
Summary: This study establishes a method for isolating and long-term maintenance of undifferentiated spermatogonia containing spermatogonial stem cells (SSCs) from goat testes.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tereza Souralova, Daniela Rehakova, Michal Jeseta, Lenka Tesarova, Jindrich Beranek, Pavel Ventruba, Ales Hampl, Irena Koutna
Summary: Human embryonic stem cells (hESCs) are being used more and more in clinical trials due to their potential to improve the outcomes of treatment for various human diseases. Ensuring the quality of hESCs is crucial, and current good manufacturing practice (cGMP) guidelines are followed during the transport of embryos and the derivation of hESC cultures. This paper presents the derivation of three clinical-grade hESC lines that meet these criteria and demonstrates the development and validation of a system for manufacturing high-quality starting materials suitable for cell therapy according to cGMP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Ziyuan Liu, Chunju He
Summary: An excellent self-healing antifouling coating with high mechanical strength was prepared through a simple one-step method. Amphiphilic block copolymer PDMS-PDMAEMA was introduced into the PDMS-PU system and zwitter-ionized during solidification, resulting in a micro-phase separation structure that improved the coating's underwater self-healing ability.
PROGRESS IN ORGANIC COATINGS
(2023)
Article
Nanoscience & Nanotechnology
Jinbao Huang, Marshall Yang, Hui Zhang, Jesse Zhu
Summary: A novel solvent-free, chemical modification-free, and mechanically, chemically, and UV robust superhydrophobic powder coating has been developed with the capability to adjust hydrophobicity. The coating exhibits remarkable mechanical stability, resistance to chemical corrosion and UV irradiation, as well as anti-abrasion and self-cleaning properties, making it promising for outdoor applications. This study contributes to the environmentally friendly preparation of robust superhydrophobic surfaces.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Delia Alba Soto, Micaela Navarro, Canbin Zheng, Michelle Margaret Halstead, Chuan Zhou, Carly Guiltinan, Jun Wu, Pablo Juan Ross
Summary: Through the use of a commercially available alternative and a chemically-defined substrate, we have successfully simplified the culture conditions for bESCs, resulting in newly derived bESC lines that are easy to establish, propagate, and maintain stability after long-term culture, while still retaining pluripotency.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Shadi Tarazi, Alejandro Aguilera-Castrejon, Carine Joubran, Nadir Ghanem, Shand Ashouokhi, Francesco Roncato, Emilie Wildschutz, Montaser Haddad, Bernardo Oldak, Elidet Gomez-Cesar, Nir Livnat, Sergey Viukov, Dmitry Lokshtanov, Segev Naveh-Tassa, Max Rose, Suhair Hanna, Calanit Raanan, Ori Brenner, Merav Kedmi, Hadas Keren-Shaul, Tsvee Lapidot, Itay Maza, Noa Novershtern, Jacob H. Flannel
Summary: Researchers have successfully generated synthetic whole embryo models with both embryonic and extraembryonic compartments using manipulated naive pluripotent stem cells. These models can complete embryonic development, develop organ progenitors, and exhibit complex structures similar to mouse embryos. This discovery highlights the potential of naive pluripotent cells.
Article
Chemistry, Physical
Antonius Chrisnandy, Delphine Blondel, Saba Rezakhani, Nicolas Broguiere, Matthias P. Lutolf
Summary: The study investigated the influence of stress relaxation of the extracellular matrix on the formation of intestinal organoids, revealing that a stress-relaxing synthetic matrix promotes crypt budding through increased symmetry breaking and niche cell formation.
Article
Engineering, Environmental
Jinbao Huang, Marshall Yang, Wenhao Zhu, Keyong Tang, Hui Zhang, Jian Chen, James J. Noel, Ivan Barker, Haiping Zhang, Jesse Zhu
Summary: This study demonstrates a novel extrusion-free method to fabricate zinc-rich powder coatings (ZRPCs) using a press instead of an extruder. The binder and zinc particles form a raspberry-like zinc-on-binder structure after press bonding, allowing for a zinc content of around 90% in the coating powders. The press-bonded zinc-rich coating films exhibit slightly lower barrier effects but have a more uniform dispersion of zinc particles, higher zinc utilization, and longer cathodic protection compared to extruded coating films with the same zinc content.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Cell & Tissue Engineering
Xiaoyong Li, Xiaoying Yao, Chunlan Mu, Zhaoxia Wang, Rong Hu, Yan Chang, Ji Wu
Summary: This study identified FGSCs in the ovaries of juvenile cynomolgus monkeys and established a long-term culture system and cell line for them. It was found that testosterone can promote self-renewal of the cells. This discovery has important implications for research in medicine and reproductive biology.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Cell & Tissue Engineering
Xiaoyong Li, Xiaoying Yao, Chunlan Mu, Zhaoxia Wang, Rong Hu, Yan Chang, Ji Wu
Summary: This study confirms the presence of FGSCs in the ovaries of juvenile cynomolgus monkeys for the first time and establishes a long-term serum- and cell feeder-free culture system and cell line.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Cell & Tissue Engineering
Tereza Souralova, Zuzana Holubcova, Drahomira Kyjovska, Ales Hampl, Irena Koutna
Summary: The research presented the generation of two sex-discordant sibling hESC lines through mechanical biopsy under xeno-and feeder-free conditions. This approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
STEM CELL RESEARCH
(2021)
Article
Materials Science, Multidisciplinary
Jeong Hui Lee, Taekyung Lim, Keumyoung Seo, Jeeyin Park, Jonguk Yang, Sang-Mi Jeong, Sanghyun Ju
Summary: The study proposes a novel method for fabricating PDMS fibers using thermal curing and stretching, without the need for molds. The resulting PDMS fibers have excellent transparency and mechanical strength, and can be functionalized for different applications.
NPG ASIA MATERIALS
(2022)
Article
Polymer Science
Gaby D. Lizana-Vasquez, Luis F. Arrieta-Viana, Janet Mendez-Vega, Aldo Acevedo, Madeline Torres-Lugo
Summary: This study focuses on the design, characterization, and evaluation of thermo-responsive synthetic terpolymers for cell manufacturing and in vitro culture applications. The terpolymers show promising potential as a scaffold for cell culture, with low cytotoxicity and the ability to encapsulate various cell types without interfering with staining or imaging protocols.
Article
Cell & Tissue Engineering
Xu Qian, Jin Koo Kim, Wilbur Tong, Luis G. Villa-Diaz, Paul H. Krebsbach
Article
Cell & Tissue Engineering
Luis G. Villa-Diaz, Jin Koo Kim, Alex Laperle, Sean P. Palecek, Paul H. Krebsbach
Review
Cell & Tissue Engineering
Paul H. Krebsbach, Luis G. Villa-Diaz
STEM CELLS AND DEVELOPMENT
(2017)
Article
Engineering, Biomedical
Xu Qian, Luis G. Villa-Diaz, Ramya Kumar, Joerg Lahann, Paul H. Krebsbach
Article
Chemistry, Multidisciplinary
Weiqiang Chen, Shuo Han, Weiyi Qian, Shinuo Weng, Haiou Yang, Yubing Sun, Luis G. Villa-Diaz, Paul H. Krebsbach, Jianping Fu
Article
Chemistry, Physical
Yubing Sun, Koh Meng Aw Yong, Luis G. Villa-Diaz, Xiaoli Zhang, Weiqiang Chen, Renee Philson, Shinuo Weng, Haoxing Xu, Paul H. Krebsbach, Jianping Fu
Article
Cell & Tissue Engineering
Guihua Jiang, Julie Di Bernardo, Michael M. Maiden, Luis G. Villa-Diaz, Omar S. Mabrouk, Paul H. Krebsbach, K. Sue O'Shea, Shaun M. Kunisaki
STEM CELLS AND DEVELOPMENT
(2014)
Article
Cell & Tissue Engineering
Luis Gerardo Villa-Diaz, Jin Koo Kim, Ierg Lahann, Paul H. Krebsbach
STEM CELLS TRANSLATIONAL MEDICINE
(2014)
Review
Cell & Tissue Engineering
Gabriele D. Bigoni-Ordonez, Daniel Czarnowski, Tyler Parsons, Gerard J. Madlambayan, Luis G. Villa-Diaz
CURRENT STEM CELL RESEARCH & THERAPY
(2019)
Review
Cell & Tissue Engineering
Christina Ramotowski, Xianggui Qu, Luis G. Villa-Diaz
STEM CELLS TRANSLATIONAL MEDICINE
(2019)
Article
Dentistry, Oral Surgery & Medicine
Zeena Qiryaqoz, Suraj Timilsina, Daniel Czarnowski, Paul H. Krebsbach, Luis G. Villa-Diaz
ORTHODONTICS & CRANIOFACIAL RESEARCH
(2019)
Article
Oncology
Tyler M. Parsons, Katie L. Buelow, Alaa Hanna, Marisa A. Brake, Crystal Poma, Sarah E. Hosch, Randal J. Westrick, Luis G. Villa-Diaz, George D. Wilson, Gerard J. Madlambayan
Summary: The study revealed that CSF-1 signaling induced differentiation of intratumoural HSPCs into M2 polarized TAMs, promoting post-RT tumour survival and regrowth. Conversely, hyperfractionated/pulsed radiation therapy (PRT) and GW2580 disrupted this process, resulting in improved tumour killing and mouse survival.
BRITISH JOURNAL OF CANCER
(2022)
Article
Multidisciplinary Sciences
S. Timilsina, T. Kirsch-Mangu, S. Werth, B. Shepard, T. Ma, L. G. Villa-Diaz
Summary: This study investigated the effects of simulated microgravity on human pluripotent stem cells. The results showed that microgravity enhanced cell proliferation and self-renewal, while affecting cell differentiation and cell cycle regulation. These findings are important for understanding the impacts of microgravity on human health and physiological processes.
Article
Cell Biology
Tugba Topal, Byoung Choul Kim, Luis G. Villa-Diaz, Cheri X. Deng, Shuichi Takayama, Paul H. Krebsbach
INTEGRATIVE BIOLOGY
(2019)
Article
Cell & Tissue Engineering
Jacob H. Jordahl, Luis Villa-Diaz, Paul H. Krebsbach, Joerg Lahann
CURRENT STEM CELL REPORTS
(2016)
