期刊
NATURE PROTOCOLS
卷 6, 期 3, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2010.200
关键词
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资金
- RCUK
- MRC
- BBSRC
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/F018371/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E500315/1] Funding Source: researchfish
- Medical Research Council [G0400551] Funding Source: researchfish
- BBSRC [BB/F018371/1] Funding Source: UKRI
- MRC [G0400551] Funding Source: UKRI
Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, for spike timing-dependent plasticity clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real-time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments through an intuitive and powerful interface with a minimal initial lead time of a few hours. After initial configuration, experimental results can be generated within minutes of establishing cell recording.
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