Article
Geriatrics & Gerontology
Xiaolan Lian, Mingyi Zhao, Ji Xia, Yulei He, Lina Zhang
Summary: The study found that both well-known mRNA splicing genes and rarely reported mRNA splicing relevant genes were significantly down-regulated during the aging process of HSPCs. This research enhanced our understanding of the role of mRNA splicing in HSPC aging.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2021)
Article
Infectious Diseases
Chak-Lun Chan, Hogan Kok-Fung Wai, Peng Wu, Siu-Wai Lai, Olivia Sinn-Kay Chan, Hein M. Tun
Summary: This study presents a methodology for simultaneous extraction and detection of antibiotic residues in animal and environmental samples. It can qualitatively detect 30 different antibiotics and has the potential for quantification of antibiotic residues.
Article
Environmental Sciences
Lucie Drabova, Darina Dvorakova, Katerina Urbancova, Tomas Gramblicka, Jana Hajslova, Jana Pulkrabova
Summary: Interference of residual lipids is a common problem in ultratrace analysis of contaminants in fatty matrices. This study evaluated the efficiency and performance of four commonly used sorbents for lipid clean-up and found that the EMR-lipid sorbent was the most efficient for the determination of organic pollutants in fatty fish samples.
Article
Biochemistry & Molecular Biology
Yue Yu, Hui-Yu Pan, Xin Zheng, Fang Yuan, Ying-Lin Zhou, Xin-Xiang Zhang
Summary: Early cancer diagnosis is crucial for successful treatment and prognosis. This study presents an ultrasensitive quantification method for the simultaneous detection of multiple modified nucleosides. The method was applied to breast tissue samples from breast cancer patients, revealing significant changes in the abundance of certain modified nucleosides, suggesting their potential as cancer biomarkers.
Article
Chemistry, Analytical
Sara Kheirandish, Shokoufeh Goudarzi, Maryam Amirahmadi, Shiva Ghareghani, Homanaz Ghafari, Bahram Daraei, Mahmoud Ghazi-Khansari
Summary: This study aimed to detect antibiotic residues in Iranian yogurt samples using a selective multi-residues method. The results showed no antibiotic contamination in the samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Martin Meyer, Lidia Montero, Sven W. Meckelmann, Oliver J. Schmitz
Summary: This study compared the analysis of carbohydrates using four common chromatographic methods coupled to mass spectrometry. It was found that gas chromatography and reversed-phase liquid chromatography, after derivatisation, showed superior separation performance compared to the other methods. Targeted analysis using different working modes of the mass spectrometer improved sensitivity and repeatability, with RP-LC-MS in MRM mode after derivatisation being the most suitable method for monosaccharide analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Feng Xu, Yuanyuan Pang, Qixing Nie, Zhipeng Zhang, Chuan Ye, Changtao Jiang, Yuan Wang, Huiying Liu
Summary: Pyrimidines are essential nutrients and key molecules in nucleic acid biosynthesis and metabolism. The concept of pyrimidine metabolome was proposed, covering 14 analytes and establishing a novel analytical strategy. Metabolomics analysis revealed diverse pyrimidine metabolism and offered reference data for future studies.
Article
Toxicology
Wei Zhang, Ruiguo Wang, John P. Giesy, Su Zhang, Shulin Wei, Peilong Wang
Summary: In this study, the effects of TDCIPP on immune cells were investigated using iTRAQ-based proteomic techniques. A total of 180 differentially expressed proteins were identified, with some found to be involved in pathways related to apoptosis, DNA damage, cell cycle arrest, immune-toxicity, and signaling pathways. The study also suggested that the collapse of MMP may play a role in TDCIPP induced apoptosis.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Ting Huang, Mateusz Staniak, Felipe da Veiga Leprevost, Amanda M. Figueroa-Navedo, Alexander R. Ivanov, Alexey I. Nesvizhskii, Meena Choi, Olga Vitek
Summary: Repeated measures experimental designs are commonly used in mass spectrometry-based proteomics to quantify proteins in biological subjects repeatedly over multiple conditions or times. Combining repeated measures with tandem mass tag (TMT) labeling presents statistical challenges due to unique interplays of variation. This manuscript proposes a family of linear mixed-effects models for analyzing proteomics experiments with repeated measures and TMT multiplexing, which decompose the variation and enable statistical inference of differential protein abundance.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Plant Sciences
Jialiang Li, Rui Yang, Yonglei Jiang, Shubin Sun, Junying Li, Hao Gu, Ying Lin, Xianxue Luo, Chenggang He, Yi Chen
Summary: This study provides insights into the responses of tobacco roots to different soil types, showing that red soil promotes the coordination of chemical composition of tobacco leaves and the growth and activity of the root system, while limestone soil and paddy soil have relatively weaker effects.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Nafiseh Bagheri, Haider A. J. Al Lawati, Nada A. Al Sharji, Javad Hassanzadeh
Summary: The 2D metal-organic framework (MOF) proved to be an efficient adsorbent for simultaneous extraction of fluoroquinolones (FQs). The use of acetic acid solution for elution showed high recovery without the need for toxic organic solvents. The method demonstrated great selectivity and sensitivity in detecting OF, NOR, and CIP in milk samples.
Article
Multidisciplinary Sciences
Tzu-Yun Kuo, Jen-Hung Wang, Yung-Wen Huang, Ting-Yi Sung, Ching-Tai Chen
Summary: In this study, a novel quantitation pipeline that combines sequence database searching, spectral library searching, and a feature-based peptide-spectrum-match (PSM) filter is proposed to improve the quantitation accuracy of isobaric labeling-based mass spectrometry. The results demonstrate that this pipeline can enhance the overall quantitation accuracy at both the PSM and protein levels. This is the first study to utilize spectral library searching to improve isobaric labeling-based quantitation.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Salvador Gonzalez-Gordo, Jose M. Palma, Francisco J. Corpas
Summary: The proteomic composition of peroxisomes in sweet pepper fruits was analyzed, revealing significant differences in peroxisomal proteins involved in antioxidant metabolism and beta-oxidation pathway. These findings provide new insights into the metabolic functions of peroxisomes during fruit ripening.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Applied
Harischandra R. Naik, M. S. Pallavi, M. Bheemanna, K. PavanKumar, V. Chandra Sekhara Reddy, Udaykumar R. Nidoni, M. Paramasivam, Satish Yadav
Summary: A method for simultaneous determination of 79 pesticide residues in pigeonpea has been developed and validated, with low LOD and LOQ, good recoveries, and applicability for routine monitoring purposes.
Article
Biochemical Research Methods
Rongmei Huang, Yi Hong, Yike Wu, Weifeng Li, Wenlan Liu
Summary: We developed a new LC-MS/MS method for simultaneous determination of serum testosterone and free testosterone levels. The method is simple to perform and can be applied in clinical practice, providing important information for diagnosis, treatment, and monitoring of androgen-related diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Geriatrics & Gerontology
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Stefano Patassini, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper
Summary: The study found alterations in levels of several metals across multiple regions of Parkinson's disease dementia (PDD) brain, with widespread decreases in copper being the most common, closely resembling those in Alzheimer's disease dementia (ADD) patients, suggesting similar disease mechanisms in both dementias.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Ophthalmology
Izabela P. Klaska, Anne White, Pilar Villacampa, Justin Hoke, Laura Abelleira-Hervas, Ryea N. Maswood, Robin R. Ali, Catey Bunce, Richard D. Unwin, Garth J. S. Cooper, Paul N. Bishop, James W. Bainbridge
Summary: Intravitreal administration of recombinant human opticin can safely protect against the development of pathological angiogenesis and promote its regression in mice. This finding suggests the potential use of opticin as a therapeutic intervention for ocular diseases.
EXPERIMENTAL EYE RESEARCH
(2022)
Letter
Multidisciplinary Sciences
Richard D. Unwin, Daniela S. Couto, Simon J. Clark
Article
Geriatrics & Gerontology
Sasha A. Philbert, Jingshu Xu, Melissa Scholefield, Stephanie J. Church, Richard D. Unwin, Garth J. S. Cooper
Summary: Vascular dementia (VaD) is the second most common cause of age-related dementia, and there are currently no disease-modifying therapies available for VaD. A study investigating VaD found elevated levels of sodium and sodium/potassium ratio, as well as decreased levels of potassium in the hippocampal tissue. These findings suggest dysfunction of Na+/K+-exchanging ATPase, possibly due to deficient ATP generation. The study highlights the potential diagnostic importance of measuring cerebral sodium in VaD patients.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Cell Biology
Helene A. Fachim, Zohaib Iqbal, J. Martin Gibson, Ivona Baricevic-Jones, Amy E. Campbell, Bethany Geary, Akheel A. Syed, Antony Whetton, Handrean Soran, Rachelle P. Donn, Adrian H. Heald
Summary: After metabolic bariatric surgery, changes in inflammatory markers and circulating proteins were observed, with some proteins correlating with alterations in IL6, TNF alpha, and CRP. Further studies are required to elucidate the mechanisms by which MBS reduces low-grade inflammation.
Article
Geriatrics & Gerontology
Sasha A. Philbert, Jingshu Xu, Stephanie J. Church, Richard D. Unwin, Federico Roncaroli, Garth J. S. Cooper
Summary: This study used inductively coupled plasma-mass spectrometry to measure the levels of essential metals in vascular dementia (VaD) brain tissue. The results showed elevated sodium levels, decreased potassium levels, and increased Na/K ratios in VaD brain tissue. These findings suggest a possible involvement of altered Na+/K+ exchanging ATPase activity in the pathogenesis of VaD.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Geriatrics & Gerontology
Crystal Sang, Sasha A. Philbert, Danielle Hartland, Richard. D. Unwin, Andrew W. Dowsey, Jingshu Xu, Garth J. S. Cooper
Summary: Sporadic Alzheimer's disease (sAD) is a common cause of age-related neurodegeneration and dementia. This study suggests that a deficiency of vitamin B5 may impair brain energy metabolism in sAD by affecting the levels of mitochondrial-TCA cycle enzymes.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Biochemical Research Methods
Nyasha Munjoma, Giorgis Isaac, Ammara Muazzam, Olivier Cexus, Fowz Azhar, Hardev Pandha, Anthony D. Whetton, Paul A. Townsend, Ian D. Wilson, Lee A. Gethings, Robert S. Plumb
Summary: Lipids are crucial in biological processes and their accurate measurement is essential for understanding diseases and human health. A high throughput LC-MS method was developed to screen over 2000 lipids in human plasma/serum, and it combines the advantages of global lipid analysis and targeted approaches.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Immunology
Anna L. Tierney, Wajd Mohammed Alali, Thomas Scott, Karen S. Rees-Unwin, Simon J. Clark, Richard D. Unwin
Summary: The SARS-CoV-2 virus continues to cause significant morbidity and mortality worldwide. Dysregulation of the complement cascade, specifically elevations in FHR proteins, may predict disease severity in COVID-19 patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Sasha A. Philbert, Sarah J. Schoenberger, Jingshu Xu, Stephanie J. Church, Richard D. Unwin, Garth J. S. Cooper
Summary: The study found that hippocampal copper levels were significantly elevated in patients with T2D, similar to untreated cases of Wilson's disease (WD), while hippocampal copper levels were markedly deficient in patients with sAD. These results suggest that hippocampal copper may play a role in the neurodegeneration and cognitive impairment in T2D, and therapeutic approaches targeting copper reduction, similar to those used in WD, may be applicable to patients with T2D.
Article
Archaeology
K. N. White, D. Chiasserini, R. Loynes, A. R. David, B. E. van Dongen, K. Drosou, R. Forshaw, S. Fraser, P. Causey-Freeman, J. Metcalfe, E. Murphy, M. Regan, P. J. Reimer, D. G. Tosh, A. Whetton, A. J. Freemont
Summary: Scientific examination of mummies provides insights into human ancestry, diet, disease, cultures, and societies. However, invasive methods have raised concerns. A minimally invasive approach using imaging techniques and biopsy sampling allows for the collection of valuable data without significant disturbance to the mummy. This study on Takabuti demonstrates the potential of such methods.
JOURNAL OF ARCHAEOLOGICAL SCIENCE-REPORTS
(2023)
Article
Oncology
Kelechi Njoku, Andrew Pierce, Bethany Geary, Amy E. Campbell, Janet Kelsall, Rachel Reed, Alexander Armit, Rachel Da Sylva, Liqun Zhang, Heather Agnew, Ivona Baricevic-Jones, Davide Chiasserini, Anthony D. Whetton, Emma J. Crosbie
Summary: This study aimed to identify urine-based proteomic signatures that can differentiate endometrial cancer patients from symptomatic controls. Through mass spectrometry and machine learning techniques, a 10-marker protein panel was discovered that accurately detected endometrial cancer. This study provides a potential urine-based detection tool for non-invasive endometrial cancer detection.
BRITISH JOURNAL OF CANCER
(2023)
Article
Oncology
Ammara Muazzam, Matt Spick, Olivier N. F. Cexus, Bethany Geary, Fowz Azhar, Hardev Pandha, Agnieszka Michael, Rachel Reed, Sarah Lennon, Lee A. Gethings, Robert S. Plumb, Anthony D. Whetton, Nophar Geifman, Paul A. Townsend
Summary: Despite ongoing research, effective tools for detection and monitoring of prostate cancer are still lacking. This study aims to identify non-invasive biomarkers that can complement PSA in clinical decision-making. Novel prostate cancer protein signatures were discovered using an advanced analytical technique, and the results were validated using a second cohort of patients. The study provides a proteomic signature that can enhance the diagnosis and risk assessment of localized prostate cancer.
Article
Neurosciences
Sasha A. Philbert, Jingshu Xu, Melissa Scholefield, Stefano Patassini, Stephanie J. Church, Richard D. Unwin, Federico Roncaroli, Garth J. S. Cooper
Summary: Vascular dementia (VaD) is a common type of dementia in the elderly, but its molecular basis is not well understood compared to other age-related dementias. This study used a novel technique to measure urea levels in post-mortem brain tissue and found elevated levels in multiple regions of the brain in VaD cases, suggesting a common urea-mediated disease mechanism present in several types of age-related dementias.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Chemistry, Analytical
Stephanie Church, Kimme L. Hyrich, Kayode Ogungbenro, Richard D. Unwin, Anne Barton, James Bluett
Summary: We developed a novel HPLC MS/MS assay for the quantification of tofacitinib, which showed the potential to sensitively measure biochemical adherence. This method has the potential to be a more objective measure that could be used in the future in the clinic, but further studies are needed to explore factors that may influence drug level measurement, such as patients' clinical characteristics.
ANALYTICAL METHODS
(2023)