Article
Biochemistry & Molecular Biology
Hong-Lim Kim, Tae-Ryong Riew, Jieun Park, Youngchun Lee, In-Beom Kim
Summary: Researchers have developed a new method by combining light and electron microscopy with immunolabeling, to overcome technical barriers in immuno-electron microscopy. This method allows for excellent antigenicity of target proteins and preservation of ultrastructure, without the need for specifically designed correlative light and electron microscopy devices. It can be easily applied in many biomedical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Monika Kakol, Ezher Tagliasacchi, Andrzej Borkowski, Miroslaw Slowakiewicz
Summary: The nutritional condition of seed mussels used in New Zealand's Greenshell mussel aquaculture industry shows high variability, which may contribute to the marked variation observed in spat performance. Environmental factors that limit feeding during harvest and transport of spat are likely influencing the quality of spat used by the industry. Measures to improve the quality of wild spat supply, such as testing nutritional condition and developing nursery culture methods and artificial spat feeds, are needed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dong Si, Jason Chen, Andrew Nakamura, Luca Chang, Haowen Guan
Summary: The study of macromolecular structures has revolutionized the pharmaceutical industry's development of new vaccines. While X-ray crystallography has been the traditional method, cryo-EM is gaining popularity due to recent advancements. Automated software is desired for labor-intensive de novo macromolecular complex modeling. This paper presents deep learning-based methods for macromolecule modeling applications.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Erney Ramirez-Aportela, Jose M. Carazo, Carlos Oscar S. Sorzano
Summary: Single-particle cryo-electron microscopy is a powerful technique for determining the 3D structure of biological molecules. This article explores the use of deepEMhancer, a deep-learning approach, as a regularizer in the refinement process. The combination of deepEMhancer with RELION improves image quality and is particularly useful for studying membrane proteins.
Article
Multidisciplinary Sciences
Hongcheng Fan, Bo Wang, Yan Zhang, Yun Zhu, Bo Song, Haijin Xu, Yujia Zhai, Mingqiang Qiao, Fei Sun
Summary: The amphiphilic low-molecular-weight protein hydrophobin (HFBI) can self-assemble into a crystalline monolayer film, which is used as a support film in cryo-EM to protect specimens and overcome the preferred orientation problem. By adjusting the buffer pH, the distribution of particle orientations can be regulated. This support film has been successfully applied to determine high-resolution structures of various specimens in cryo-EM.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yuanmin Zhu, Qing Zhang, Xuming Yang, Menghao Li, Biao Wang, Meng Gu
Summary: The study utilized ultra-low-dose cryo-TEM to characterize the atomic-scale structure of organic-inorganic hybrid perovskite nanoplatelets, revealing the growth pattern and crystal structure evolution. Insights gained from atomic-scale investigations may have significant impacts on the development of battery materials and solar energy technologies.
Article
Chemistry, Multidisciplinary
Hanglong Wu, Teng Li, Sai P. Maddala, Zafeiris J. Khalil, Rick R. M. Joosten, Brahim Mezari, Emiel J. M. Hensen, Gijsbertus de With, Heiner Friedrich, Jeroen A. van Bokhoven, Joseph P. Patterson
Summary: A distributed electron microscopy method is proposed for studying complex chemical reactions, demonstrated through studying the desilication mechanism of zeolite crystals. The reaction is shown to proceed via a two-step anisotropic etching process, with defects in curved surfaces and between subunits controlling desilication kinetics.
Article
Biochemical Research Methods
Xuefeng Du, Haohan Wang, Zhenxi Zhu, Xiangrui Zeng, Yi-Wei Chang, Jing Zhang, Eric Xing, Min Xu
Summary: In this study, a Hybrid Active Learning (HAL) framework is proposed to alleviate the labeling burden by querying a large pool of unlabeled subtomograms. HAL framework utilizes uncertainty sampling and a discriminator to optimize subtomogram selection, achieving comparable testing performance with less labeled resources.
Review
Biochemistry & Molecular Biology
Miguel Ricardo Leung, Tzviya Zeev-Ben-Mordehai
Summary: The different oligomeric forms of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) have defined cellular and subcellular distributions. While crystal structures of AChE and BChE have been available for many years, structures of the physiologically relevant cholinesterase tetramer were only recently solved by cryo-electron microscopy (cryo-EM). The next frontier in cholinesterase structural biology is to image membrane-anchored ChE oligomers directly in their native environment - the cell.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yuqing Ye, Keni Cota-Ruiz, Jesus M. Cantu, Carolina Valdes, Jorge L. Gardea-Torresdey
Summary: Engineered nanomaterials interact dynamically with matrices such as soil, water, and biological tissues throughout their lifecycle. Electron microscopy plays a crucial role in assessing these interactions, providing high-resolution imaging capabilities for real-time visualization of nanomaterials in biological matrices. Recent progress in evaluating nanobio interactions using EM has led to advancements in nanoresearch, shedding light on nanocorona formation, lipid nanoparticle analysis, and real-time observation of cellular uptake of nanoparticles.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Materials Science, Multidisciplinary
Akira Sugawara, Tetsuya Akashi, Yoshio Takahashi, Toshiaki Tanigaki
Summary: Magnetic imaging with nanometer resolution is important for controlling the magnetization configuration within magnetic materials to have an impact on various fields of technology. This paper describes three recent topics in magnetic imaging research using ultra-high-voltage, cold-field-emission transmission electron microscopes.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Z. Faidon Brotzakis, Thomas Lohr, Michele Vendruscolo
Summary: Research using cryo-electron microscopy has identified the process of SARS-CoV-2 spike transitioning from closed to open state, revealing a cryptic pocket that could be targeted for therapeutic interventions. This study emphasizes the importance of understanding transient intermediate states of proteins for developing effective treatments against invisible binding sites.
Article
Chemistry, Physical
Jin-Hu Dou, Maxx Q. Arguilla, Yi Luo, Jian Li, Weizhe Zhang, Lei Sun, Jenna L. Mancuso, Luming Yang, Tianyang Chen, Lucas R. Parent, Grigorii Skorupskii, Nicole J. Libretto, Chenyue Sun, Min Chieh Yang, Phat Vinh Dip, Edward J. Brignole, Jeffrey T. Miller, Jing Kong, Christopher H. Hendon, Junliang Sun, Mircea Dinca
Summary: Researchers have successfully grown single crystals of 2D metal-organic frameworks up to 200 µm in size by balancing in-plane and out-of-plane interactions, revealing anisotropic transport and a clear correlation between conductivity and the nature of the metal cation in the sheets.
Article
Neurosciences
Vladan Lucic
Summary: This article reviews the importance of electron microscopy in the study of neuronal synapse structures and discusses the use of image processing software methods. Special attention is given to the application of recent cryo-electron microscopy and tomography methods in molecular level analysis.
CURRENT OPINION IN NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kayo Nozawa, Yoshimasa Takizawa, Leonidas Pierrakeas, Chizuru Sogawa-Fujiwara, Kazumi Saikusa, Satoko Akashi, Ed Luk, Hitoshi Kurumizaka
Summary: The canonical nucleosome is the major packaging unit of eukaryotic chromatin, and the H3-H4 octasome is a nucleosome-like particle with alternative histone stoichiometry. The structure of the H3-H4 octasome is flexible.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)