期刊
NATURE PHOTONICS
卷 7, 期 10, 页码 806-810出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NPHOTON.2013.245
关键词
-
资金
- Broad Stem Cell Research Center at UCLA Innovation Award
Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the trade-off between imaging speed and sensitivity(1) are problematic for acquiring blur-free images of fast phenomena, such as sub-millisecond biochemical dynamics in live cells and tissues(2), and cells flowing at high speed(3). Here, we report a technique that achieves real-time pixel readout rates that are one order of magnitude faster than a modern electron multiplier charge-coupled device-the gold standard in high-speed fluorescence imaging technology(4). Termed fluorescence imaging using radiofrequency-tagged emission (FIRE), this approach maps the image into the radiofrequency spectrum using the beating of digitally synthesized optical fields. We demonstrate diffraction-limited confocal fluorescence imaging of stationary cells at a frame rate of 4.4 kHz, and fluorescence microscopy in flow at a velocity of 1 m s(-1), corresponding to a throughput of approximately 50,000 cells per second.
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