期刊
NATURE NEUROSCIENCE
卷 16, 期 9, 页码 1266-U146出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.3473
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资金
- Wellcome Trust
- Medical Research Council
- MRC [MR/J002976/1] Funding Source: UKRI
- Medical Research Council [MR/J002976/1] Funding Source: researchfish
Regulation of calcium-permeable AMPA receptors (CP-AMPARs) is crucial in normal synaptic function and neurological disease states. Although transmembrane AMPAR regulatory proteins (TARPs) such as stargazin (gamma-2) modulate the properties of calciumimpermeable AMPARs (CI-AMPARs) and promote their synaptic targeting, the TARP-specific rules governing CP-AMPAR synaptic trafficking remain unclear. We used RNA interference to manipulate AMPAR-subunit and TARP expression in gamma-2-lacking stargazer cerebellar granule cells-the classic model of TARP deficiency. We found that TARP gamma-7 selectively enhanced the synaptic expression of CP-AMPARs and suppressed CI-AMPARs, identifying a pivotal role of gamma-7 in regulating the prevalence of CP-AMPARs. In the absence of associated TARPs, both CP-AMPARs and CI-AMPARs were able to localize to synapses and mediate transmission, although their properties were altered. Our results also establish that TARPed synaptic receptors in granule cells require both gamma-2 and gamma-7 and reveal an unexpected basis for the loss of AMPAR-mediated transmission in stargazer mice.
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