Article
Biochemistry & Molecular Biology
Eirini Filidou, Leonidas Kandilogiannakis, Gesthimani Tarapatzi, Michail Spathakis, Colin Su, Alin Rai, David W. Greening, Konstantinos Arvanitidis, Vasilis Paspaliaris, George Kolios
Summary: Pluripotent stem cells play a vital role in regenerative medicine. However, the limited availability and ethical concerns associated with embryonic pluripotent stem cells have led to the discovery of very small embryonic-like (VSEL) stem cells. In this study, a simplified and effective method for isolating small pluripotent stem cells from human peripheral blood is presented. The isolated small blood stem cells (SBSC) population expresses pluripotency markers and also exhibits characteristics of hematopoietic and mesenchymal stem cells. Proteomic profiling reveals the presence of various stem cell markers and transcription regulatory complex factors in SBSCs. This novel isolation process yields a abundant population of small-sized cells with pluripotent characteristics from human peripheral blood.
Article
Biochemistry & Molecular Biology
Olga A. Krasnova, Vitaly V. Gursky, Alina S. Chabina, Karina A. Kulakova, Larisa L. Alekseenko, Alexandra V. Panova, Sergey L. Kiselev, Irina E. Neganova
Summary: This study analyzed the morphology of human pluripotent stem cells to identify the best clones for further clinical applications. By analyzing different cell lines and phenotypes, informative markers were identified to separate the best clones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Takuya Hirose, Jun'ichi Kotoku, Fujio Toki, Emi K. Nishimura, Daisuke Nanba
Summary: DeepACT is a deep learning-based automated cell tracking technology for quality control and identification of cultured human stem cells. It can analyze cell motion and spatial information to evaluate keratinocyte dynamics accurately.
Article
Engineering, Biomedical
Bin Jiang, Weijie Li, Samantha Stewart, Wenquan Ou, Baolin Liu, Pierre Comizzoli, Xiaoming He
Summary: The study found that natural sand can seed ice above -10 degrees C, allowing hiPSCs to be cryopreserved without serum and with reduced cryoprotectants, leading to high cell survival. The cryopreserved hiPSCs also retained high pluripotency and functions.
BIOACTIVE MATERIALS
(2021)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Biophysics
Hao Chen, Bin Jiang, James G. Shamul, Xiaoming He
Summary: The beating pattern of 3D iPSC-CSs can be conveniently detected and quantified by calculating the relative change of entropy in all frames/images of non-fluorescent optical signal without labeling any cells. This label-free method enables rapid detection and quantification of the beating pattern of each iPSC-CS, as well as reliable detection of their responses to anticancer and cardiac drugs.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Ines Milagre, Carolina Pereira, Raquel A. Oliveira
Summary: Human pluripotent stem cells (PSCs), including embryonic and induced pluripotent stem cells, are widely used in biomedical research for understanding development, disease progression, and drug discovery. However, concerns have been raised regarding their safety in regenerative medicine, particularly due to the occurrence of aneuploid cells resulting from errors in chromosome segregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Jiesi Luo, Yuyao Lin, Xiangyu Shi, Guangxin Li, Mehmet H. Kural, Christopher W. Anderson, Matthew W. Ellis, Muhammad Riaz, George Tellides, Laura E. Niklason, Yibing Qyang
Summary: Research successfully derived functional xenogeneic-free hiPSC-VSMCs by replacing animal-derived reagents with functional counterparts of human origin, and developed tissue rings with comparable mechanical strength. Engineered vascular tissues were generated by seeding XF-hiPSC-VSMCs onto biodegradable PGA scaffolds, suitable for implantation into an immunodeficient mice model.
ACTA BIOMATERIALIA
(2021)
Correction
Cell & Tissue Engineering
Ran Zheng, Ting Geng, Dan-Ya Wu, Tianzhe Zhang, Hai-Nan He, Hai-Ning Du, Donghui Zhang, Yi-Liang Miao, Wei Jiang
Summary: A new method has been developed to convert human embryonic stem cells and induced pluripotent stem cells into EPSCs using chemicals, demonstrating extended pluripotency in terms of molecular features, chimeric ability, and transcriptome. Certain chemicals targeting glycolysis and histone methyltransferase have been identified to facilitate the conversion and maintenance of feeder-free EPSCs, providing insights for further research and applications in the field of pluripotency.
Article
Engineering, Biomedical
Jiesi Luo, Xiangyu Shi, Yuyao Lin, Yifan Yuan, Mehmet H. Kural, Juan Wang, Matthew W. Ellis, Christopher W. Anderson, Shang-Min Zhang, Muhammad Riaz, Laura E. Niklason, Yibing Qyang
Summary: The study successfully developed xenogeneic-free conditions for deriving functional endothelial cells from human induced pluripotent stem cells (XF-hiPSC-ECs), which exhibited similar marker expression and function to human primary ECs. Moreover, the XF-hiPSC-ECs responded to shear stress with typical cell alignment and gene expression, and were successfully used to endothelialize decellularized human vessels in a dynamic bioreactor system, advancing the potential clinical application of tissue engineered vascular grafts.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Olga A. Krasnova, Karina A. Kulakova, Julia V. Sopova, Evgenyi Y. Smirnov, Sergey A. Silonov, Ekaterina V. Lomert, Olga A. Bystrova, Marina G. Martynova, Irina E. Neganova
Summary: G-protein-coupled receptors (GPCRs) are the largest family of cell surface receptors and play important roles in diverse developmental processes. However, their role in pluripotency maintenance and acquisition during reprogramming towards hiPSCs has been overlooked. Through RNA interference screening, adhesion GPCR GPR123 was identified as a potential reprogramming effector. Suppression of GPR123 leads to loss of pluripotency and differentiation in hPSCs, suggesting its essential role in maintaining and acquiring pluripotency.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Chemistry, Multidisciplinary
Carlos Honrado, Nadine Michel, John H. Moore, Armita Salahi, Veronica Porterfield, Michael J. McConnell, Nathan S. Swami
Summary: The translation of human-induced pluripotent stem cells into human neural progenitor cells has potential applications in drug discovery and transplant therapy for neurological diseases. By using electrophysiology for quantification of hNPC subpopulations, it is possible to control cell composition for optimal organoid development. This label-free detection strategy allows for faster optimization of cellular compositions and prevents loss of cell viability.
Article
Immunology
Kibaek Choe, Yusaku Hontani, Tianyu Wang, Eric Hebert, Dimitre G. Ouzounov, Kristine Lai, Ankur Singh, Wendy Beguelin, Ari M. Melnick, Chris Xu
Summary: Intravital three-photon microscopy allows for the visualization of immune cell behavior in mouse lymph nodes at depths of 600-900 μm. This technique enables measurement of migration ability in different depths and the observation of inflammation effects on T cell motility.
Article
Engineering, Biomedical
Pamela L. Graney, Zhe Zhong, Sarah Post, Ilana Brito, Ankur Singh
Summary: This study reports the use of maleimide-functionalized poly(ethylene glycol) hydrogels as immune organoids to develop early memory B-cell phenotype and germinal center-like B cells. By using specific cytokines and bacterial antigen presentation, primary murine B cells can be converted to an early memory phenotype in ex vivo immune organoids.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biology
Jessica Elmore, Chavez Carter, Amie Redko, Nicholas Koylass, Amelia Bennett, Max Mead, Marinel Ocasio-Rivera, Weishan Huang, Ankur Singh, Avery August
Summary: The kinase ITK is normally required for the differentiation of Th17 cells, but this study shows that under certain inflammatory conditions, ITK can be bypassed.
COMMUNICATIONS BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Valeria M. Juarez, Alyssa N. Montalbine, Ankur Singh
Summary: Current drug discovery and therapeutic delivery approaches have not fully utilized the complex regulatory network of the microbiome, which is associated with diseases and interacts with drugs. Understanding the interplay between microbes and drugs can lead to the development of new therapeutic strategies and changes in drug delivery methods.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Chemistry, Analytical
Guillaume Aubry, Hyun Jee Lee, Hang Lu
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Fobang Liu, Tianchang Xu, Nga Lee Ng, Hang Lu
Summary: This study reveals that oxidative stress may be a mechanism through which ambient fine particulate matter exerts adverse biological effects. It also demonstrates the association between cellular health and ROS levels upon exposure to secondary organic aerosols, emphasizing the role of oxidative stress in the biological effects of SOA exposure.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yen-Yu Hsu, Agnes M. Resto Irizarry, Jianping Fu, Allen P. Liu
Summary: Plasma membrane tension acts as a regulator of cellular activities, but current measurement techniques are limited in their ability to investigate membrane biophysics and the role of tension. In this study, a new optical reporter for membrane tension is developed using a modified E. coli mechanosensitive channel and circularly permuted GFP, which allows measurement of membrane tension based on fluorescence intensity changes.
Article
Biophysics
Benteng Ma, Xinya Liu, Zhuoyu Zhang, Chao Ma, Rashik Chand, Saee Patwardhan, Chuanyu Wang, Soracha D. Thamphiwatana, Pengyu Chen, Weiqiang Chen
Summary: This study used a digital nanoplasmonic microarray immunosensor combined with a microfluidic biomimetic Leukemia-on-a-Chip model to monitor cytokine secretion dynamics during CD19 CAR T-cell therapy against precursor B-cell acute lymphocytic leukemia (B-ALL). The results revealed a heterogeneous secretion profile of various cytokines during CAR T-cell therapy and confirmed a correlation between the cytokine secretion profile and the CAR T-cell cytotoxic activity.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Jiacheng He, Siqi Wu, Wu Chen, Albert Kim, Wen Yang, Chuanyu Wang, Zhengyang Gu, Jialiang Shen, Siyuan Dai, Weiqiang Chen, Pengyu Chen
Summary: Immunomodulation therapies are crucial for immune-related diseases, and precise real-time detection of cytokines is essential. This study developed a label-free multiplex immunosensor using nanoplasmonic bioink, which can be easily fabricated and applied for real-time detection of multiple cytokines.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Feng Lin, Xia Li, Shiyu Sun, Zhongyi Li, Chenglin Lv, Jianbo Bai, Lin Song, Yizhao Han, Bo Li, Jianping Fu, Yue Shao
Summary: Researchers have developed an efficient biomaterial system that can generate gut spheroids from human pluripotent stem cells through mechanically enhanced tissue morphogenesis. The study also reveals the mechanisms behind gut spheroid formation, including the effects of cell multilayering and crowding, and proposes a mechanism for morphogenetic transitions.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Zachary C. Stevenson, Megan J. Moerdyk-Schauwecker, Stephen A. Banse, Dhaval S. Patel, Hang Lu, Patrick C. Phillips
Summary: High-throughput transgenesis using synthetic DNA libraries is a powerful method for exploring genetic function. However, the need for library transgenesis has restricted its application to single-cell models. In this study, we present TARDIS, a method that overcomes these limitations and enables large-scale transgenesis in multicellular systems. We demonstrate its utility in Caenorhabditis elegans and show that transformation yields can be increased up to approximately 1000-fold compared to current methods.
Article
Biochemical Research Methods
Omotola Okesanjo, Guillaume Aubry, Sven Behrens, Hang Lu, J. Carson Meredith
Summary: Surfactant-free capillary foams (CFs) are highly tolerant to oil and have unique stability and flow properties. They can flow through a microporous environment and recover a large percentage of foam pumped through it. CF flow lacks steady state and exhibits phenomena such as increasing apparent viscosity, reconfigurable flow paths, and intermittent clogging of the micromodel from oil-particle composites and trapped bubbles in pores. CFs surprisingly exhibit similar bubble generation and destruction mechanisms to classical foams despite the absence of surfactants.
Article
Geriatrics & Gerontology
Mei ElGindi, Jiranuwat Sapudom, Anna Garcia Sabate, Brian Chesney Quartey, Aseel Alatoom, Mohamed Al-Sayegh, Rui Li, Weiqiang Chen, Jeremy Teo
Summary: Microgravity accelerates aging and reduces immune response in both aged individuals and astronauts. This study investigates the effects of simulated microgravity on dendritic cells (DCs) cultured in collagen hydrogels, revealing that both aging or loose tissue and exposure to simulated microgravity alter the immunogenicity of DCs.
Article
Cell Biology
Ngoc Luu, Apratim Bajpai, Rui Li, Seojin Park, Mahad Noor, Xiao Ma, Weiqiang Chen
Summary: This study reveals the defective mechanosensation of vascular smooth muscle cells (VSMCs) during aging, leading to a decline in their ability to sense and adapt to mechanical perturbations. Aged VSMCs exhibit a relatively inert mechanobiological state with altered actin cytoskeletal integrity, resulting in impaired mechanosensitivity and dynamic mechanoresponse. This decline in mechanosensation is mediated by hyperactivity of Piezo1-dependent calcium signaling. Inhibition of Piezo1 can alleviate vascular aging and partially restore the loss in dynamic contractile properties in aged cells.
Article
Engineering, Biomedical
Liana Kramer, Hannah W. Song, Kaiya Mitchell, Mythili Kartik, Ritika Jain, Victoria Lozano Escarra, Enrique Quiros, Harrison Fu, Ankur Singh, Krishnendu Roy
Summary: High-affinity antigen-specific B cells are generated within specialized structures called germinal centers (GCs) in lymphoid organs. Follicular dendritic cells (FDCs) present antigens on their membrane surface to activate B cells, leading to their rapid proliferation and differentiation. Researchers have used liposomal membranes as antigen-presenting FDC-like fluid lipid surface in ex vivo studies to investigate this process. Their findings suggest that fluid membrane-bound antigens can induce a strong GC response and provide a novel synthetic in vitro system for studying GC biology and expanding therapeutic B cells.
ADVANCED NANOBIOMED RESEARCH
(2022)
