Review
Chemistry, Analytical
Luis Abraham Garcia-Hernandez, Eduardo Martinez-Martinez, Denni Pazos-Solis, Javier Aguado-Preciado, Ateet Dutt, Abraham Ulises Chavez-Ramirez, Brian Korgel, Ashutosh Sharma, Goldie Oza
Summary: This paper reviews recent developments in the detection of cancer cells using the microfluidics-based lab-on-a-chip method, with a focus on techniques that use optical phenomena and various probes for sensing, amplification, and immobilization. The paper also discusses current challenges and prospects for cancer diagnosis.
Article
Chemistry, Analytical
Abdallah M. Alkhaiyat, Mohamed Badran
Summary: This study proposes a lab-on-chip design that efficiently separates circulating tumor cells (CTCs) from white blood cells (WBCs) and blood platelets (PLTs). Numerical simulations are used to optimize the design parameters, and the effects of applied voltage values and buffer inlet velocity are investigated. The proposed design achieves 100% separation efficiency in simulation and shows potential for early detection of CTCs and personalized cancer treatment.
Article
Chemistry, Analytical
Yu-Shih Chen, Tzu-Wei Lo, Hong-Yuan Huang, Lien-Min Li, Yi-Wen Wang, Da-Jeng Yao, Wen-Syang Hsu, Cheng-Hsien Liu
Summary: The combination of In Vitro Fertilization (IVF) and microfluidic technology offers a promising solution to infertility by enhancing the success rate of IVF and accelerating embryo growth and development. By utilizing a microfluidic embryo lab chip for co-cultivation, researchers observed faster embryo growth rates and higher blastocyst development rates compared to traditional culture methods.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Multidisciplinary
Samaneh Zare Harofte, Madjid Soltani, Saeed Siavashy, Kaamran Raahemifar
Summary: Nowadays, artificial intelligence creates numerous opportunities in the life sciences. Integrating AI with microfluidics can significantly impact various fields of biotechnology.
Article
Biochemical Research Methods
Ning Zhang, Chao Yue, Xiaobo Zhan, Zhi Cheng, Chao Li, Yaohua Du, Feng Tian
Summary: The quantitative analysis of respiratory viruses is important for diagnosis, medicine and prognosis. In this study, a lab-on-a-chip platform combined with an automated control system was used to achieve quantitative analysis of COVID-19 pseudovirus from large-volume nasal swab samples. The method can be extended to other respiratory viruses and sample types.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Sangam Srikanth, U. S. Jayapiriya, Satish Kumar Dubey, Arshad Javed, Sanket Goel
Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed for simultaneous cultivation and detection of bacteria, with high accuracy in quantifying bacterial concentration and validating viability, as well as real-time and on-field applications.
Article
Materials Science, Multidisciplinary
Zhenzhen Chen, Liang Shen, Xiong Zhao, Hongqiang Chen, Yaxuan Xiao, Yonghai Zhang, Xiaoping Yang, Jinhua Zhang, Jinjia Wei, Nanjing Hao
Summary: Microfluidics, especially acoustofluidics, offers new opportunities in chemical and biomedical engineering by manipulating fluids at the micrometer or submicrometer scale. This review discusses the utility of acoustofluidic technology, summarizes different types of acoustic micromixers, highlights their applications in various fields, and identifies the practical challenges that need to be addressed. Overall, this review provides valuable insights for both academic research and industrial applications.
APPLIED MATERIALS TODAY
(2022)
Review
Pharmacology & Pharmacy
Renata Maia, Violeta Carvalho, Rui Lima, Graca Minas, Raquel O. Rodrigues
Summary: This systematic review summarizes the recent progress in the use of microneedles (MNs) in biomedical applications, including drug delivery, biomarker detection, and their potential integration with microfluidic devices. Although MNs have been extensively utilized in lab-on-a-chip platforms, recent studies have also explored their applicability in organ-on-a-chip models. Overall, the presence of MNs in advanced microfluidic devices offers simplified drug delivery, microinjection, and biomarker detection through integrated biosensors, enabling real-time monitoring of various biomarkers in lab- and organ-on-a-chip platforms.
Article
Biochemistry & Molecular Biology
Emanuele Piccinno, Anna Grazia Monteduro, Francesco Dituri, Silvia Rizzato, Gianluigi Giannelli, Giuseppe Maruccio
Summary: The study validated a miniaturized on-chip platform for real-time, non-destructive cell proliferation assays and drug screening, comparing its effectiveness with the traditional 96-well plate assay. Results showed the new platform's efficacy comparable to the standard method, with advantages in reagent consumption, operator time, and costs, potentially accelerating drug development processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Canan Ozyurt, Inci Uludag, Bahar Ince, Mustafa Kemal Sezginturk
Summary: Lab-on-a-chip (LOC) or micro total analysis system is a microfluidic technology that integrates and automates analytical laboratory procedures into a single instrument or chip. This article reviews the recent developments in the application of LOC biosensors for cancer detection. Microfluidics has revolutionized healthcare diagnosis by enabling the reconstruction of functional organ units on chips and studying human diseases like cancer. In the past five years, LOCs have been widely used in various biomedical applications such as bioassays, cell analysis, drug discovery, and personalized medicine. This review provides an overview of microfluidic-based cancer research, including its pros, cons, and future prospects.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Environmental Sciences
V Karthik, B. Karuna, P. Senthil Kumar, A. Saravanan, R. Hemavathy
Summary: Recently, the decrease in water availability and quality due to industrialization and anthropogenic activities has led to heavy metals becoming significant pollutants in the water environment. Conventional monitoring techniques have drawbacks, and biosensors have emerged as reasonable devices for detecting environmental contaminants. This review summarizes the toxic effect, source, and occurrence of heavy metals, and discusses the use of lab on a chip with suitable recognition materials for heavy metal detection. The lab on a chip is a promising micro-electrical mechanical system device that enables fast, cost-effective, and highly sensitive analysis of heavy metals.
Article
Chemistry, Analytical
Stefan Gassmann, Till Schleifer, Helmut Schuette
Summary: This paper presents a Lab-on-a-chip platform for colorimetric measurements in water, providing an effective method for monitoring nutrients in open water. The platform can be customized for different analytes by changing reagents and detection wavelength.
Article
Biochemistry & Molecular Biology
Gulsun Bagci, Dehan Comez, Hande Topel, Yeliz Yilmaz, Ezgi Bagirsakci, Aysim Gunes, Gizem Bati Ayaz, Ismail Tahmaz, Muge Bilgen, Gulhas Solmaz, Devrim Pesen Okvur, Nese Atabey
Summary: This study investigated the role of aberrant c-Met activation in regulating the extravasation and homing capacity of hepatocellular carcinoma (HCC) cells using a Lab on a Chip device. It was found that c-Met overexpression and activation directed HCC cells towards the hepatocytes-enriched microenvironment, and blocking c-Met phosphorylation attenuated the extravasation and homing capacity of HCC cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Chemistry, Analytical
Alexandro Catini, Rosamaria Capuano, Giuseppe Tancredi, Giulio Dionisi, Davide Di Giuseppe, Joanna Filippi, Eugenio Martinelli, Corrado Di Natale
Summary: This paper presents an automatic chemical assay platform for continuous monitoring of harmful compounds in aquaculture. The platform uses a lab-on-a-chip approach and low-cost optoelectronic components to analyze water samples, providing a reliable and cost-effective solution.
Article
Chemistry, Physical
Cristina Chircov, Alexandra Catalina Birca, Alexandru Mihai Grumezescu, Bogdan Stefan Vasile, Ovidiu Oprea, Adrian Ionut Nicoara, Chih-Hui Yang, Keng-Shiang Huang, Ecaterina Andronescu
Summary: The study introduced a new method for the synthesis of magnetite nanoparticles using a microfluidic lab-on-chip device to achieve controlled properties. It investigated the influence of iron precursor solution concentration and flow on the final properties of the nanomaterials, ultimately achieving uniformity and stability in the synthesized nanoparticles.
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Editorial Material
Biochemical Research Methods
Tal Nawy
Review
Biochemistry & Molecular Biology
Orit Rozenblatt-Rosen, Aviv Regev, Philipp Oberdoerffer, Tal Nawy, Anna Hupalowska, Jennifer E. Rood, Orr Ashenberg, Ethan Cerami, Robert J. Coffey, Emek Demir, Li Ding, Edward D. Esplin, James M. Ford, Jeremy Goecks, Sharmistha Ghosh, Joe W. Gray, Justin Guinney, Sean E. Hanlon, Shannon K. Hughes, E. Shelley Hwang, Christine A. Iacobuzio-Donahue, Judit Jane-Valbuena, Bruce E. Johnson, Ken S. Lau, Tracy Lively, Sarah A. Mazzilli, Dana Pe'er, Sandro Santagata, Alex K. Shalek, Denis Schapiro, Michael P. Snyder, Peter K. Sorger, Avrum E. Spira, Sudhir Srivastava, Kai Tan, Robert B. West, Elizabeth H. Williams
Article
Biotechnology & Applied Microbiology
Sitara Persad, Zi-Ning Choo, Christine Dien, Noor Sohail, Ignas Masilionis, Ronan Chaligne, Tal Nawy, Chrysothemis C. C. Brown, Roshan Sharma, Itsik Pe'er, Manu Setty, Dana Pe'er
Summary: Metacells are identified using an algorithm called SEACells, which overcomes the sparsity and retains heterogeneity of single-cell data. SEACells outperforms existing algorithms in identifying comprehensive, compact, and well-separated metacells in both RNA and ATAC modalities across datasets. This method is particularly well suited for patient cohorts and allows for improved gene-peak associations, ATAC gene scores computation, and inference of critical regulators' activities during differentiation. Our metacells reveal expression dynamics and chromatin landscape reconfiguration during hematopoietic differentiation and identify CD4 T cell differentiation and activation states associated with COVID-19 disease onset and severity.
NATURE BIOTECHNOLOGY
(2023)
