Counting stem cells: methodological constraints

Lentiviral vector common integration sites in preclinical models and a clinical trial reflect a benign integration bias and not oncogenic selection

(2011) A. Biffi et al.   BLOOD

Polyclonal fluctuation of lentiviral vector-transduced and expanded murine hematopoietic stem cells

(2011) T. Maetzig et al.   BLOOD

Is normal hematopoiesis maintained solely by long-term multipotent stem cells?

(2011) M. Cavazzana-Calvo et al.   BLOOD

Dynamic variation in cycling of hematopoietic stem cells in steady state and inflammation

(2011) Hitoshi Takizawa et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Evolution of human BCR–ABL1 lymphoblastic leukaemia-initiating cells

(2011) Faiyaz Notta et al.   NATURE

Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding

(2011) Rong Lu et al.   NATURE BIOTECHNOLOGY

Dynamics of gene-modified progenitor cells analyzed by tracking retroviral integration sites in a human SCID-X1 gene therapy trial

(2010) G. P. Wang et al.   BLOOD

Cellular barcoding tool for clonal analysis in the hematopoietic system

(2010) A. Gerrits et al.   BLOOD

Hematopoietic Stem Cell Heterogeneity: Subtypes, Not Unpredictable Behavior

(2010) Timm Schroeder   Cell Stem Cell

Integration profile of retroviral vector in gene therapy treated patients is cell-specific according to gene expression and chromatin conformation of target cell

(2010) Luca Biasco et al.   EMBO Molecular Medicine

Some hematopoietic stem cells are more equal than others: Figure 1.

(2010) Hanno Hock   JOURNAL OF EXPERIMENTAL MEDICINE

Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment

(2010) Yohei Morita et al.   JOURNAL OF EXPERIMENTAL MEDICINE

High-Throughput, Sensitive Quantification of Repopulating Hematopoietic Stem Cell Clones

(2010) S. Kim et al.   JOURNAL OF VIROLOGY

Genetic variegation of clonal architecture and propagating cells in leukaemia

(2010) Kristina Anderson et al.   NATURE

Transfusion independence and HMGA2 activation after gene therapy of human β-thalassaemia

(2010) Marina Cavazzana-Calvo et al.   NATURE

Sustained high-level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector-transduced CD34+ cells

(2009) Y.-J. Kim et al.   BLOOD

Comprehensive genomic access to vector integration in clinical gene therapy

(2009) Richard Gabriel et al.   NATURE MEDICINE

Gene Therapy for Immunodeficiency Due to Adenosine Deaminase Deficiency

(2009) Alessandro Aiuti et al.   NEW ENGLAND JOURNAL OF MEDICINE

Long-Term Vector Integration Site Analysis Following Retroviral Mediated Gene Transfer to Hematopoietic Stem Cells for the Treatment of HIV Infection

(2009) Jun Hayakawa et al.   PLoS One

Hematopoietic Stem Cell Gene Therapy with a Lentiviral Vector in X-Linked Adrenoleukodystrophy

(2009) N. Cartier et al.   SCIENCE

Genomic DNA k-mer spectra: models and modalities

(2009) Benny Chor et al.   GENOME BIOLOGY

Potential genotoxicity from integration sites in CLAD dogs treated successfully with gammaretroviral vector-mediated gene therapy

(2008) M Hai et al.   GENE THERAPY

Dissecting T cell lineage relationships by cellular barcoding

(2008) Koen Schepers et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Methods for integration site distribution analyses in animal cell genomes

(2008) Angela Ciuffi et al.   METHODS

DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer

(2008) Gary P. Wang et al.   NUCLEIC ACIDS RESEARCH