期刊
NATURE METHODS
卷 9, 期 11, 页码 1088-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2184
关键词
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资金
- US National Science Foundation, Synthetic Biology Engineering Research Center
- Miller Institute for Basic Scientific Research
- US Department of Defense through the National Defense Science and Engineering Graduate Fellowship
Bacterial regulators of transcriptional elongation are versatile units for building custom genetic switches, as they control the expression of both coding and noncoding RNAs, act on multigene operons and can be predictably tethered into higher-order regulatory functions (a property called composability). Yet the less versatile bacterial regulators of translational initiation are substantially easier to engineer. To bypass this tradeoff, we have developed an adaptor that converts regulators of translational initiation into regulators of transcriptional elongation in Escherichia coli. We applied this adaptor to the construction of several transcriptional attenuators and activators, including a small molecule-triggered attenuator and a group of five mutually orthogonal riboregulators that we assembled into NOR gates of two, three or four RNA inputs. Continued application of our adaptor should produce large collections of transcriptional regulators whose inherent composability can facilitate the predictable engineering of complex synthetic circuits.
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