期刊
NATURE METHODS
卷 8, 期 8, 页码 684-U120出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1643
关键词
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资金
- European Commission [PIRG-GA-2007-209789, LSHG-CT-2007-037897]
- Columbia University
- McKnight Innovation in Neuroscience Award
- Czech government [MSM6007665808, MSM6007665801, AVOZ60870520]
- EU [CZ.1.07/2.3.00/09.0203]
- University of South Bohemia
Membrane proteins are a large, diverse group of proteins, serving a multitude of cellular functions. They are difficult to study because of their requirement of a lipid membrane for function. Here we show that two-photon polarization microscopy can take advantage of the cell membrane requirement to yield insights into membrane protein structure and function, in living cells and organisms. The technique allows sensitive imaging of G-protein activation, changes in intracellular calcium concentration and other processes, and is not limited to membrane proteins. Conveniently, many suitable probes for two-photon polarization microscopy already exist.
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