期刊
NATURE METHODS
卷 6, 期 10, 页码 729-732出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1367
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资金
- National Institute of Biomedical Imaging and Bioengineering
- Eunice Kennedy Shriver National Institute
- Child Health and Human Development of the National Institutes of Health
- US National Research Council
Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.
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