期刊
NATURE IMMUNOLOGY
卷 11, 期 8, 页码 759-U130出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ni.1903
关键词
-
类别
资金
- Deutsche Forschungsgemeinschaft [SFB746, SFB 620]
- German Federal and State Governments [GSC-4]
Developing B cells express distinct classes of B cell antigen receptors (BCRs) that differ in their heavy chain (HC). Although only mu HC is expressed in early stages, delta HC-containing BCRs dominate on the surface of mature B cells. The reason for the tightly regulated expression of these receptors is poorly understood. Here we show that mu HC was specifically required for precursor BCR (pre-BCR) function and that delta HC was unable to form a functional pre-BCR. A conserved asparagine (N)-linked glycosylation site at position 46 (N46) in the first conserved domain of mu HC was absolutely required for pre-BCR function, and swapping that domain with delta HC resulted in a functional delta HC-containing pre-BCR. When tested in the context of the BCR, mu HC with a mutant N46 showed normal function, which indicated that N46-glycosylation is specifically required for pre-BCR function. Our results suggest an unexpected mode of pre-BCR function, in which binding of the surrogate light chain to N46 mediates autonomous crosslinking and, concomitantly, receptor formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据