期刊
NATURE CHEMICAL BIOLOGY
卷 14, 期 9, 页码 887-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41589-018-0114-4
关键词
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资金
- NIH [R01GM112940, F31GM119357, T32 GM88118, P41GM111244]
- National Institutes of Health (NIH) [P41 GM103403, S10 RR029205]
- DOE Office of Science [DE-AC02-06CH11357]
- DOE Office of Biological and Environmental Research [KP1605010, KC0401040]
The ykkC family of bacterial riboswitches combines several widespread classes that have similar secondary structures and consensus motifs but control different genes in response to different cellular metabolites. Here we report the crystal structures of two distinct ykkC riboswitches specifically bound to their cognate ligand ppGpp, a second messenger involved in stress response, or PRPP, a precursor in purine biosynthesis. Both RNAs adopt similar structures and contain a conserved core previously observed in the guanidine-specific ykkC riboswitch. However, ppGpp and PRPP riboswitches uniquely employ an additional helical element that joins the ends of the ligand-sensing domains and creates a tunnel for direct and Mg2+-mediated binding of ligands. Mutational and footprinting experiments highlight the importance of conserved nucleotides forming the tunnel and long-distance contacts for ligand binding and genetic response. Our work provides new insights into the specificity of riboswitches and gives a unique opportunity for future studies of RNA evolution.
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