Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Endocrinology & Metabolism
Eira Karvonen, Kai J. E. Krohn, Annamari Ranki, Annika Hau
Summary: APECED is a severe and incurable autoimmune disease that can be treated with stem cell therapy. The study shows that patient-derived iPSCs have stem cell properties and can be used for gene correction, confirming their pluripotency and potential therapeutic use.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell & Tissue Engineering
Hiroki Tsutsumi, Ryota Kurimoto, Ryo Nakamichi, Tomoki Chiba, Takahide Matsushima, Yuta Fujii, Risa Sanada, Tomomi Kato, Kana Shishido, Yuriko Sakamaki, Tsuyoshi Kimura, Akio Kishida, Hiroshi Asahara
Summary: This study successfully generated a tendon-like tissue using induced pluripotent stem cells, which has potential clinical applications.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Medicine, Research & Experimental
Rina Hashimoto, Ayaka Sakamoto, Sayaka Deguchi, Renxing Yi, Emi Sano, Akitsu Hotta, Kazutoshi Takahashi, Shinya Yamanaka, Kazuo Takayama
Summary: This study investigated the roles of neuropilin-1, basigin, TMPRSSs, and CTSs in SARS-CoV-2 infection using the CRISPR interference system and ACE2-expressing human iPS cells. Double knockdown of TMPRSS2 and CTSB significantly reduced the viral load. Combination of CTPB and TMPRSS2 inhibitors showed highly efficient antiviral effects against various SARS-CoV-2 variants, independent of gender.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Summer A. Helmi, Leili Rohani, Ahmed R. Zaher, Youssry M. El Hawary, Derrick E. Rancourt
Summary: Bone healing is a well-organized process regulated by multiple factors. The Notch signaling pathway plays a significant role in controlling bone mesenchymal stem cell differentiation. Inhibiting the Notch pathway can greatly enhance the differentiation of both embryonic and induced pluripotent stem cells, potentially improving overall healing outcomes. Stem cell transplantations are prominent candidates for treating critical-sized bone defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Antonio Ferreira, Jon C. Aster
Summary: Notch receptors participate in a conserved signaling pathway that has diverse effects on normal cells and is highly dependent on the cellular context and signaling dosage. Notch plays different roles in various types of cancers, acting as either an oncogene or a tumor suppressor and affecting tumor cells and stromal elements in multiple ways. This review provides an overview of the roles of Notch in cancer and discusses the challenges and potential approaches for clinical translation of Notch targeting agents.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Samantha J. Linder, Tiziano Bernasocchi, Barbara Martinez-Pastor, Kelly D. Sullivan, Matthew D. Galbraith, Caroline A. Lewis, Christina M. Ferrer, Ruben Boon, Giorgia G. Silveira, Hyo Min Cho, Charles Vidoudez, Stuti Shroff, Joao P. Oliveira-Costa, Kenneth N. Ross, Rami Massri, Yusuke Matoba, Eugene Kim, Bo R. Rueda, Shannon L. Stott, Eyal Gottlieb, Joaquin M. Espinosa, Raul Mostoslavsky
Summary: This study reveals an adaptive pathway in cancer cells that allows them to survive under glutamine starvation by downregulating proline biosynthesis and promoting de novo glutamine synthesis. This pathway highlights a previously unrecognized metabolic plasticity of cancer cells and suggests a potential target for developing metabolism-driven anticancer therapies.
Article
Gastroenterology & Hepatology
Yang He, Pengzhan He, Shimin Lu, Weiguo Dong
Summary: In this study, it was found that KIFC3 was upregulated in gastric cancer and correlated with higher T stage and poor prognosis. Overexpression of KIFC3 promoted the proliferation and metastasis of gastric cancer cells, and this effect was mediated by the activation of the Notch1 pathway.
DIGESTIVE AND LIVER DISEASE
(2023)
Article
Cell & Tissue Engineering
Wei Shan, Qin Yu, Yan Long, Qian Luo, Honghu Li, Yingli Han, Yulin Xu, Shan Fu, Xiangjun Zeng, Cong Wei, Yang Gao, Xiaoqing Li, Xia Li, Lifei Zhang, Lizhen Liu, Ming Chen, Pengxu Qian, He Huang
Summary: This study established an in vitro differentiation system that successfully generated hematopoietic cells with in vivo hematopoietic reconstitution potential from mouse PSCs. The combination of 3D induction system and OP9 stromal cell coculture yielded c-kit(+) cells with superior repopulating activity.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Bin Liu, Dinghui Wang, Tianhua Xiong, Yajie Liu, Xiaodong Jing, Jianlin Du, Qiang She
Summary: This study revealed that EPCs are a source of epicardial adipocytes, showing lipid droplet accumulation under adipogenic induction. Activation of the Notch signaling pathway attenuated adipogenic differentiation of EPCs, while inhibition promoted differentiation.
STEM CELLS INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Sudipta Mahato, Trupti Agrawal, Divya Pidishetty, Savitri Maddileti, Vinay Kumar Pulimamidi, Indumathi Mariappan
Summary: This protocol describes a simplified, robust, and stepwise method of generating retinal organoids using a hybrid culture system. The resulting organoids contain proliferating, multipotent retinal precursors that self-assemble to form mature retinal organoids.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Biochemistry & Molecular Biology
Nattaphong Rattanavirotkul, Kristina Kirschner, Tamir Chandra
Summary: Senescence is a cellular stress response triggered by diverse stressors and can act as a tumor suppressor mechanism, with the ability to be transmitted to neighboring cells. Recent evidence suggests a more complex scenario for secondary senescence, involving juxtacrine communication between cells, and differences have been noted between primary and secondary senescent endpoints.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Environmental Sciences
Yong Wang, Wen Li, Fangmei Huang, Xiaojian Wu, Wenbin Chen, Ming Dong, Jie Zhou
Summary: The study demonstrates that SCA inhibits the proliferation of myeloma cells by promoting pro-apoptotic proteins and targeting the expression of Notch3. This suggests that SCA may have potential for further development as a treatment for multiple myeloma.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Biology
LinXi Cheng, MingHui Xie, WeiHua Qiao, Yu Song, YanYong Zhang, YingChao Geng, WeiLin Xu, Lin Wang, Zheng Wang, Kai Huang, NianGuo Dong, YuHua Sun
Summary: This study describes a two-step chemically defined xeno-free method for generating valve endothelial-like cells from human pluripotent stem cells. The derived valvular cells exhibit superior proliferative and clonogenic potential, and could be used as seed cells for construction of valve organoids or next generation tissue engineered heart valves.
COMMUNICATIONS BIOLOGY
(2021)
Article
Developmental Biology
Yan Li, Chao Tang, Fan Liu, Caiying Zhu, Feng Liu, Ping Zhu, Lu Wang
Summary: This study investigates the role of DNA methyltransferase 1 (Dnmt1) in hematopoietic stem and progenitor cell (HSPC) generation. It finds that Dnmt1 is essential for HSPC generation by repressing Notch signaling through DNA methylation.
Article
Cardiac & Cardiovascular Systems
Naveed Akbar, Adam T. Braithwaite, Emma M. Corr, Graeme J. Koelwyn, Coen van Solingen, Clement Cochain, Antoine-Emmanuel Saliba, Alastair Corbin, Daniela Pezzolla, Malene Moller Jorgensen, Rikke Baek, Laurienne Edgar, Carla De Villiers, Mala Gunadasa-Rohling, Abhirup Banerjee, Daan Paget, Charlotte Lee, Eleanor Hogg, Adam Costin, Raman Dhaliwal, Errin Johnson, Thomas Krausgruber, Joey Riepsaame, Genevieve E. Melling, Mayooran Shanmuganathan, Christoph Bock, David R. F. Carter, Keith M. Channon, Paul R. Riley, Irina A. Udalova, Kathryn J. Moore, Daniel Anthony, Robin P. Choudhury
Summary: Acute myocardial infarction leads to a rapid increase in blood neutrophils. While chemokine elevation has been considered their source, this study shows that endothelial cell activation can release extracellular vesicles (EVs) that promote mobilization of neutrophils from the spleen to peripheral blood. These EVs carry VCAM-1 and miRNA-126, and their accumulation in the spleen induces inflammatory gene expression and mobilizes splenic neutrophils.
CARDIOVASCULAR RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Pabola S. Nascimento, Marcelo T. Moura, Roberta L. Oliveira-Silva, Pamela Ramos-Deus, Claudio C. Bartolomeu, Ana M. Benko-Iseppon, Jose C. Ferreira-Silva, Antonio S. Santos Filho, Marcos A. L. Oliveira
Summary: This study investigates the correlations between PATF gene expression patterns and mRNA regulatory features in sheep embryos. It identifies different PATF gene expression patterns and reveals their associations with mRNA 3'UTR length, polyadenylation sites, and m6A sites.
Article
Biochemistry & Molecular Biology
Abel Vertesy, Oliver L. Eichmueller, Julia Naas, Maria Novatchkova, Christopher Esk, Meritxell Balmana, Sabrina Ladstaetter, Christoph Bock, Arndt von Haeseler, Juergen A. Knoblich
Summary: This study reveals that organoids experience cellular stress, but it is limited to specific subpopulations and does not affect neuronal specification or maturation. The researchers have developed an algorithm to identify and remove stressed cells, proving its usefulness in different organoid systems.
Article
Biotechnology & Applied Microbiology
Fabian Amman, Rudolf Markt, Lukas Endler, Sebastian Hupfauf, Benedikt Agerer, Anna Schedl, Lukas Richter, Melanie Zechmeister, Martin Bicher, Georg Heiler, Petr Triska, Matthew Thornton, Thomas Penz, Martin Senekowitsch, Jan Laine, Zsofia Keszei, Peter Klimek, Fabiana Naegele, Markus Mayr, Beatrice Daleiden, Martin Steinlechner, Harald Niederstaetter, Petra Heidinger, Wolfgang Rauch, Christoph Scheffknecht, Guenther Vogl, Gunther Weichlinger, Andreas Otto Wagner, Katarzyna Slipko, Amandine Masseron, Elena Radu, Franz Allerberger, Niki Popper, Christoph Bock, Daniela Schmid, Herbert Oberacher, Norbert Kreuzinger, Heribert Insam, Andreas Bergthaler
Summary: Wastewater surveillance of SARS-CoV-2 at the national scale is effective in tracking emerging variants. The study developed a system called VaQuERo, which allows for accurate quantification of predefined variants in complex wastewater samples. The research also identified increased viral genetic diversity during the Delta variant period and proposed a framework for predicting emerging variants and measuring the reproductive advantage of variants of concern using wastewater data.
NATURE BIOTECHNOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Katja Kivinen, Henri G. A. M. van Luenen, Myriam Alcalay, Christoph Bock, Joanna Dodzian, Katerina Hoskova, Danielle Hoyle, Ondrej Hradil, Sofie Kjellerup Christensen, Bernhard Korn, Theodoros Kosteas, Monica Morales, Krzysztof Skowronek, Vasiliki Theodorou, Geert Van Minnebruggen, Jean Salamero, Lavanya Premvardhan
Summary: Core facilities are crucial in the field of life sciences as they generate data and ensure quality standards. It is important to appropriately acknowledge or cite their contributions in research papers.
Article
Cell Biology
Tamara Casteels, Simon Bajew, Jiri Reinis, Lennart Enders, Michael Schuster, Frederic Fontaine, Andre C. Mueller, Bridget K. Wagner, Christoph Bock, Stefan Kubicek
Summary: The study identifies the key factor Smndc1 connecting splicing and chromatin remodeling to the control of insulin expression in human and mouse islet cells, demonstrating its repressive role on insulin expression and regulation of the β-cell transcription factor Pdx1.
Article
Biology
Antigoni Gogolou, Celine Souilhol, Ilaria Granata, Filip J. Wymeersch, Ichcha Manipur, Matthew Wind, Thomas J. R. Frith, Maria Guarini, Alessandro Bertero, Christoph Bock, Florian Halbritter, Minoru Takasato, Mario R. Guarracino, Anestis Tsakiridis
Summary: The neural crest (NC) is an important cell population in embryonic development, and understanding its regionalization is crucial. This study investigates the acquisition of posterior axial identity in NC cells derived from neuromesodermal progenitors (NMPs) through the regulation of transcription factors and signaling pathways. The findings reveal a previously unknown role of TBXT in influencing posterior NC fate and highlight the existence of cell type-dependent modes of posterior axial identity control.
Article
Immunology
Ram Vinay Pandey, Johanna Strobl, Anna Redl, Luisa Unterluggauer, Laura Gail, Lisa Kleissl, Sophie Mueller, Denise Atzmueller, Victoria Fife-Gernedl, Thomas Krausgruber, Hanna Knaus, Margit Mitterbauer, Philipp Wohlfarth, Werner Rabitsch, Christoph Bock, Georg Stary
Summary: Allogeneic hematopoietic stem-cell transplantation (HSCT) aims to rebuild the host's immune system using donor stem cells. However, there are complications that can affect the success of HSCT, such as leukemia relapse or graft-versus-host-disease (GvHD). This study investigated the regulatory processes involved in T cell recovery after HSCT, using multi-omics analysis of T cells from the skin and blood. The findings revealed the importance of epigenetic regulation and identified specific epigenetic regulators in restoring T cell homeostasis after transplantation.
CLINICAL IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laura Boccuni, Elke Podgorschek, Moritz Schmiedeberg, Ekaterini Platanitis, Peter Traxler, Philipp Fischer, Alessia Schirripa, Philipp Novoszel, Angel R. Nebreda, J. Simon C. Arthur, Nikolaus Fortelny, Matthias Farlik, Veronika Sexl, Christoph Bock, Maria Sibilia, Pavel Kovarik, Mathias Mueller, Thomas Decker
Summary: This study reveals the synergistic effect of cell stress-induced p38 MAPK signaling and IFN-stimulated signal transduction, and the impact of this synergy on ISG expression and macrophage infection ability.
Article
Multidisciplinary Sciences
Johanna Klughammer, Daria Romanovskaia, Amelie Nemc, Annika Posautz, Charlotte A. Seid, Linda C. Schuster, Melissa C. Keinath, Juan Sebastian Lugo Ramos, Lindsay Kosack, Ann Evankow, Dieter Printz, Stefanie Kirchberger, Bekir Erguener, Paul Datlinger, Nikolaus Fortelny, Christian Schmidl, Matthias Farlik, Kaja Skjaerven, Andreas Bergthaler, Miriam Liedvogel, Denise Thaller, Pamela A. Burger, Marcela Hermann, Martin Distel, Daniel L. Distel, Anna Kuebber-Heiss, Christoph Bock
Summary: This study maps DNA methylation in 535 vertebrates and 45 invertebrates and establishes a reference dataset for cross-species analysis. It quantifies the association between DNA methylation and genomic DNA sequence throughout vertebrate evolution and reveals a deeply conserved association of DNA methylation with tissue type. The study also identifies evolutionary changes in DNA methylation at gene promoters for orthologous genes.
NATURE COMMUNICATIONS
(2023)
Article
Geriatrics & Gerontology
Isabella Pototschnig, Ursula Feiler, Clemens Diwoky, Paul W. Vesely, Thomas Rauchenwald, Margret Paar, Latifa Bakiri, Laura Pajed, Peter Hofer, Karl Kashofer, Nyamdelger Sukhbaatar, Gabriele Schoiswohl, Thomas Weichhart, Gerald Hoefler, Christoph Bock, Martin Pichler, Erwin F. Wagner, Rudolf Zechner, Martina Schweiger
Summary: In this study, CHX207 fibrosarcoma cells were used as a novel tool to study the mediators and metabolic consequences of CAC in C57BL/6 mice compared to non-cachectic MCA207-tumour-bearing mice. The results showed that IL-6 plays a crucial role in the development of CAC caused by CHX207 tumors.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Immunology
Wilhelm A. J. W. Vos, Albert L. Groenendijk, Marc J. T. Blaauw, Louise E. van Eekeren, Adriana Navas, Maartje C. P. Cleophas, Nadira Vadaq, Vasiliki Matzaraki, Jessica C. dos Santos, Elise M. G. Meeder, Janeri Froberg, Gert Weijers, Yue Zhang, Jingyuan Fu, Rob ter Horst, Christoph Bock, Rainer Knoll, Anna C. Aschenbrenner, Joachim Schultze, Linos Vanderkerckhove, Talent Hwandih, Elizabeth R. Wonderlich, Sai V. Vemula, Mike van der Kolk, Sterre C. P. de Vet, Willem L. Blok, Kees Brinkman, Casper Rokx, Arnt F. A. Schellekens, Quirijn de Mast, Leo A. B. Joosten, Marvin A. H. Berrevoets, Janneke E. Stalenhoef, Annelies Verbon, Jan van Lunzen, Mihai G. Netea, Andre J. A. M. van der Ven
Summary: This study applies a multi-omics approach to analyze a large cohort of people living with HIV on combination antiretroviral therapy (cART). The aim is to understand the dysregulations in the immune system, identify new biomarkers, and discover potential therapeutic targets. Results show that HIV-infected individuals still experience immune activation, immune aging, and increased risk of non-AIDS comorbidities. The study also assesses the latent viral reservoir and records COVID-19 infection and vaccination history.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Thomas Krausgruber, Anna Redl, Daniele Barreca, Konstantin Doberer, Daria Romanovskaia, Lina Dobnikar, Maria Guarini, Luisa Unterluggauer, Lisa Kleissl, Denise Atzmuller, Carolina Mayerhofer, Aglaja Kopf, Simona Saluzzo, Clarice X. Lim, Praveen Rexie, Thomas Weichhart, Christoph Bock, George Stary
Summary: By analyzing granulomas from sarcoidosis patients, researchers reconstructed the gene regulatory networks involved in granuloma formation. They found that granulomas adopt aspects of lymphoid organ development and involve processes related to immunometabolism, cytokine and chemokine signaling, and extracellular matrix remodeling. Three cell types, including metabolically reprogrammed macrophages, cytokine-producing Th17.1 cells, and fibroblasts with inflammatory and tissue-remodeling phenotypes, were identified as key players in granuloma formation. Inhibiting one of the identified processes attenuated granuloma formation in a mouse model of sarcoidosis.
Article
Biochemical Research Methods
Lethicia Souza Tavares, Roberta Lane Oliveira-Silva, Marcelo Tigre Moura, Jessica Barboza da Silva, Ana Maria Benko-Iseppon, Jose Vitor Lima-Filho
Summary: This study identified suitable reference genes for different conditions in a mouse model of Listeria monocytogenes and highlighted the importance of RT-qPCR in gene expression analysis.
Meeting Abstract
Oncology
Thomas Roetzer-Pejrimovsky, Barbara Kiesel, Karl-Heinz Nenning, Johanna Klughammer, Martin Rajchl, Christoph Bock, Johannes Hainfellner, Bernhard Baumann, Georg Langs, Adelheid Woehrer