期刊
NATURE CHEMICAL BIOLOGY
卷 10, 期 12, 页码 1049-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.1655
关键词
-
资金
- US National Institutes of Health (NIH)/National Institute of General Medical Sciences [R01 GM065865]
- Howard Hughes Medical Institute
- NIH National Research Service Award Postdoctoral Fellowship [F32GM099359, F32GM101751]
Probes that form covalent bonds with RNA molecules on the basis of their chemical reactivity would advance our ability to study the transcriptome. We developed a set of electrophilic activity-based RNA probes designed to react with unusually nucleophilic RNAs. We used these probes to identify reactive genome-encoded RNAs, resulting in the discovery of a 42-nt catalytic RNA from an archaebacterium that reacts with a 2,3-disubstituted epoxide at N7 of a specific guanosine. Detailed characterization of the catalytic RNA revealed the structural requirements for reactivity. We developed this catalytic RNA into a general tool to selectively conjugate a small molecule to an RNA of interest. This strategy enabled up to 500-fold enrichment of target RNA from total mammalian RNA or from cell lysate. We demonstrated the utility of this approach by selectively capturing proteins in yeast cell lysate that bind the ASH1 mRNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据