期刊
NATURE BIOTECHNOLOGY
卷 30, 期 4, 页码 336-343出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2157
关键词
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资金
- Richard M. Schulze Family Foundation
- Mayo Foundation
- Cancer Research UK
- US National Institutes of Health [R01 CA107082, R01CA130878, R01 CA132734]
- Cancer Research UK [13244] Funding Source: researchfish
Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumor-specific CD4(+) interleukin (IL)-17 recall response in vitro. We used this characteristic IL-17 response to screen the VSV-cDNA library and identified three different VSV-cDNA virus clones that, when used in combination but not alone, achieved the same efficacy against tumors as the complete parental virus library. VSV-expressed cDNA libraries can therefore be used to identify tumor rejection antigens that can cooperate to induce anti-tumor responses. This technology should be applicable to antigen discovery for other cancers, as well as for other diseases in which immune reactivity against more than one target antigen contributes to disease pathology.
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