Article
Gastroenterology & Hepatology
Yasser Fouad, Reda Elwakil, Medhat Elsahhar, Ebada Said, Shamardan Bazeed, Ahmed Ali Gomaa, Almoutaz Hashim, Enas Kamal, Mai Mehrez, Dina Attia
Summary: Debates in science can stimulate critical thinking and challenge different viewpoints, but may not directly resolve controversies. In the era of evidence-based medicine, holding evidence-based debates is crucial for settling disputes effectively.
LIVER INTERNATIONAL
(2021)
Article
Clinical Neurology
Ainara Elorza, Yamile Marquez, Jorge R. Cabrera, Jose Luis Sanchez-Trincado, Maria Santos-Galindo, Ivo H. Hernandez, Sara Pico, Juan Diaz-Hernandez, Ramon Garcia-Escudero, Manuel Irimia, Jose J. Lucas
Summary: Correction of mis-splicing events is a promising therapeutic approach for neurological diseases caused by splicing-affecting mutations. Next-generation RNA sequencing has enabled the investigation of global mis-splicing in diseased tissue to identify key pathogenic mis-spliced genes. Our new intersect-RNA-seq approach captures pathogenic global mis-splicing in the Huntington's disease striatum.
Article
Biochemistry & Molecular Biology
Liam Abrahams, Rosina Savisaar, Christine Mordstein, Bethan Young, Grzegorz Kudla, Laurence D. Hurst
Summary: The study investigates the impact of disruptions in exonic cis motifs, such as ESEs, on premature termination codon-containing transcripts. Results suggest that ESEs are prone to disruption by nonsense mutations, leading to low NAS rates. Additionally, nucleotide-based machine learning approaches can predict PTCs and pathogenic variants enriched in ESEs.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Liam Abrahams, Rosina Savisaar, Christine Mordstein, Bethan Young, Grzegorz Kudla, Laurence D. Hurst
Summary: The study shows that exonic splice enhancers (ESEs) are prone to disruptive nonsense mutations, leading to low rates of nonsense-associated alternative splicing (NAS). Pathogenic variants are enriched in ESEs, and both in and out of frame mutations to TAA, TGA or TAG are associated with exon skipping, supporting the importance of splice motif modulation in understanding the etiology of premature termination codon (PTC)-associated disease.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Bruno Sopko, Gracian Tejral, Guissepe Bitti, Marianna Abate, Martina Medvedikova, Marian Hajduch, Jan Chloupek, Jolana Fajmonova, Misa Skoric, Evzen Amler, Tomas Erban
Summary: Glyphosate, a broad-spectrum herbicide, selectively affects the shikimate pathway specific to plants and microorganisms, while also possibly interacting with eukaryotic translation elongation factor 1 subunit alpha 1 (eEF1 alpha 1). This interaction delays protein synthesis, suppressing spermatogenesis and cell growth, with potential effects on sperm count.
Article
Geology
Malcolm B. Hart
Summary: The long-standing debate on the formally defining Middle Cretaceous is examined in the context of planktic and benthic foraminifera distribution. While the planktic assemblages in the Albian and Cenomanian stages are distinct, there is a noteworthy turnover at the Cenomanian-Turonian Boundary Event (CTBE) unlike the minimal changes seen at the Aptian-Albian boundary in the benthic foraminifera.
CRETACEOUS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Fan Gao, Fang Wang, Huifen Cao, Yue Chen, Yong Diao, Philipp Kapranov
Summary: The human genome contains various noncoding transcripts that have been traditionally categorized into long or small based on their lengths. However, the functions and biological significance of most of these transcripts are still unknown. In this study, a high-throughput assay was developed to test the functionality of small noncoding RNAs (sncRNAs) by overexpressing them in human cells. Surprisingly, a significant fraction of unannotated sncRNAs were found to have biological relevance, challenging the assumption that they are merely degradation products. These findings suggest the potential existence of multiple functional sncRNAs in the small noncoding transcriptome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Guangyao Shan, Guoshu Bi, Yunyi Bian, Besskaya Valeria, Dejun Zeng, Huan Zhang, Guangyu Yao, Yi Zhang, Hong Fan, Cheng Zhan
Summary: The genomic and microenvironment characteristics differed between the cell cycle progression pathway altered/non-altered patients with LUAD, providing new insights for personalized treatment of LUAD patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Nahee Hwang, Yune Huh, Seonghyeon Bu, Kyung Jin Seo, Se Hwan Kwon, Jae-woo Kim, Bo Kyung Yoon, Hyo-Suk Ahn, Sungsoon Fang
Summary: This study conducted a time series analysis of peripheral blood mononuclear cells from a patient with BNT162b2-induced myocarditis, and found the importance of classical monocytes in the pathogenesis of myocarditis. The study also suggests that vaccination may affect monocytes and induce their infiltration into the heart.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Yi Guo, Dandan Bai, Wenqiang Liu, Yingdong Liu, Yalin Zhang, Xiaochen Kou, Jiayu Chen, Hong Wang, Xiaoming Teng, Ji Zuo, Shaorong Gao
Summary: Advanced paternal age alters the profile of transfer RNA-derived small RNAs, leading to anxiety-like behaviors in offspring. Changes in gene expression related to dopaminergic synapse and neurotrophin are observed in the cerebral cortex and hippocampus of male offspring, as well as in early-stage embryos. Up-regulated sperm tsRNA target genes are involved in neurogenesis and nervous system development, suggesting a potential contribution to intergenerational transmission of behavioral traits.
Article
Biochemistry & Molecular Biology
Jing Tong, Hongwanyu Li, Cong Zhang
Summary: In this study, the authors investigated the characteristics and roles of m(5)C modification and mRNA expression profiles in the decidua of pre-eclampsia (PE). Through comprehensive analysis, they identified 50 candidate genes linked to PE and validated their expression changes. The findings suggest that m(5)C modification and relevant mRNA expression changes may influence the pathogenesis of PE.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Yan Yang, Wenyun Yue, Nan Wang, Zicong Wang, Bingyan Li, Jun Zeng, Shigeo Yoshida, Chun Ding, Yedi Zhou
Summary: This study identified altered tsRNAs and miRNAs in vitreous humor samples of proliferative diabetic retinopathy (PDR) patients. The differential expression of these molecules may play important roles in the pathogenesis of PDR and could serve as potential therapeutic targets for the treatment of PDR.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Jose M. Murrieta-Coxca, Emanuel Barth, Paulina Fuentes-Zacarias, Ruby N. Gutierrez-Samudio, Tanja Groten, Alexandra Gellhaus, Angela Koeninger, Manja Marz, Udo R. Markert, Diana M. Morales-Prieto
Summary: Placenta accreta spectrum (PAS) is a group of pathological conditions characterized by abnormal attachment of the placenta to the uterus wall, and it is a major cause of maternal morbidity and mortality worldwide. In this study, altered miRNA expression, particularly in placenta-specific miRNA clusters, was identified in placenta accreta tissue compared to normal placental tissues. Dysregulated pathways associated with cell cycle regulation, inflammation, and invasion were also observed. The study provides miRNA:mRNA associations that could contribute to our understanding of PAS development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Neurosciences
Giorgia Tosoni, Dilara Ayyildiz, Julien Bryois, Will Macnair, Carlos P. Fitzsimons, Paul J. Lucassen, Evgenia Salta
Summary: The potential of exploiting the regenerative capacity of the human brain has gained attention as an alternative strategy for enhancing or restoring brain function in physiological aging or neurological diseases. However, there is a debate regarding whether the human brain has the ability to regenerate. Recent single-cell RNA sequencing studies in the human hippocampus have yielded conflicting results, raising concerns about the reliability of this approach. In this article, we critically discuss and re-analyze previously published single-cell transcriptomic datasets related to human adult hippocampal neurogenesis (AHN), highlighting the importance of addressing methodological, conceptual, and biological factors in studying AHN.
Article
Ophthalmology
Tanja Stachon, Mahsa Nastaranpour, Berthold Seitz, Eckart Meese, Lorenz Latta, Suphi Taneri, Navid Ardjomand, Nora Szentmary, Nicole Ludwig
Summary: This study evaluates the expression of mRNA and miRNA in the epithelium and stroma of patients with keratoconus, and identifies significant differences compared to healthy controls. The findings suggest that genes and miRNAs associated with metabolic changes and cell signaling pathways may play a role in the development of keratoconus.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)