Article
Chemistry, Multidisciplinary
Federico Dona, Cagakan Ozbalci, Andrea Paquola, Federica Ferrentino, Stephen J. Terry, Elisabeth M. Storck, Gaoge Wang, Ulrike S. Eggert
Summary: The membrane protein stomatin is found to be involved in the cytokinesis step of cell division, even though it is not a lipid biosynthetic enzyme. Depletion of stomatin leads to changes in cellular lipidomes, and exogenous phosphatidylcholines can rescue these defects.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Cell Biology
Mikolaj Ogrodnik
Summary: The field of cellular senescence research has expanded rapidly from in vitro studies of aging to animal and clinical research, focusing on the role of senescence markers in aging, disease, and anti-senescence interventions. The presence and role of these markers in cells prior to cell cycle arrest, particularly in replicative aging and in vivo conditions, are discussed. The article also explores disparities in quantifications of senescence markers in vivo and the applicational value of broadening the cellular senescence paradigm.
Review
Biochemistry & Molecular Biology
Rachana R. Borkute, Soeren Woelke, Gang Pei, Anca Dorhoi
Summary: Neutrophils play a complex role in tuberculosis, with both beneficial and detrimental effects on disease outcomes. Recent studies have revealed the phenotypic heterogeneity of neutrophils and their interactions with other cells in TB pathophysiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Hematology
Yanming Li, Scott A. LeMaire, Ying H. Shen
Summary: Single-cell RNA sequencing studies have provided new insights into the heterogeneity of vascular cell types, cellular transitions, communications among cell populations, and critical transcription factors in the vascular wall. This information enhances our understanding of the pathogenesis of vascular diseases and the identification of genes and variants associated with aneurysms within specific cell types. Challenges and future directions in the application of single-cell RNA sequencing in aortic biology and disease studies are also discussed.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Laura Yerly, Christine Pich-Bavastro, Jeremy Di Domizio, Tania Wyss, Stephanie Tissot-Renaud, Michael Cangkrama, Michel Gilliet, Sabine Werner, Francois Kuonen
Summary: By utilizing single-cell and spatial transcriptomics, this study identifies the cellular contributors and their transcriptional reprogramming in the spatial organization of the invasive niche in basal cell carcinomas. The findings demonstrate the potential of integrated multi-omics approaches in deciphering cancer-specific invasive properties and developing targeted therapies.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Lakmini Senavirathna, Cheng Ma, Ru Chen, Sheng Pan
Summary: The integrated spectral library-based single-cell proteomics platform developed in this study allows for identification of over 2000 proteins in a single cell, distinguishing proteome landscapes associated with cellular types and heterogeneity. By characterizing normal and cancerous pancreatic ductal cells, a significant upregulation of multiple protein networks in cancer hallmarks was identified in the cancerous cells, functionally discriminating them from normal cells. This unique platform, built on high-resolution MS and widely accepted proteomic software, has great potential for community-wide applications.
Review
Chemistry, Multidisciplinary
Sumit Kumar, Mamata Karmacharya, Yoon-Kyoung Cho
Summary: Cells are the fundamental units of life and contain the necessary information for building living creatures. Advances in synthetic biology and cell engineering allow for the redesign of biological systems, but creating life-like structures from nonliving building blocks remains challenging. Cell membrane-engineered micro or nanoreactors have promising applications.
Article
Engineering, Biomedical
Amar Tamra, Amel Zedek, Marie-Pierre Rols, David Dubuc, Katia Grenier
Summary: This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells subjected to electrochemotherapy. The biosensor offers a non-destructive and label-free technique to extract dielectric parameters and detect the effects of chemical treatments. The results show a high correlation rate and demonstrate the reliability and efficacy of the technique for biology research and personalized medicine.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2022)
Editorial Material
Multidisciplinary Sciences
Jeffrey A. Farrell
Summary: CellOracle, a computational tool, can predict the interaction of gene networks in programming cell identity during embryonic development. This tool will contribute to a better understanding of the regulation of development.
Article
Biochemistry & Molecular Biology
Yosui Nojima, Masahiko Aoki, Suyong Re, Hidekazu Hirano, Yuichi Abe, Ryohei Narumi, Satoshi Muraoka, Hirokazu Shoji, Kazufumi Honda, Takeshi Tomonaga, Kenji Mizuguchi, Narikazu Boku, Jun Adachi
Summary: This study investigated the anti-tumor efficacy of apatinib against gastric cancer cell lines and found that it differed among different cell lines. The study also revealed that apatinib acts as a multi-kinase inhibitor, predominantly targeting c-Kit. These findings contribute to a deeper understanding of the mechanism of action of apatinib in gastric cancer cells.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Editorial Material
Multidisciplinary Sciences
Alain Chedotal
Summary: The GPC3-Unc5 protein complex is found to regulate cell migration in embryos and cancer, indicating a similarity in the migration mechanisms of developing neurons and metastatic cancer cells.
Review
Plant Sciences
Abbas Shojaee, Michelle Saavedra, Shao-shan Carol Huang
Summary: Advancements in single-cell genomics, particularly in single-cell transcriptome profiling, have revolutionized our understanding of the relationship between genes and cellular functions, structures, and phenotypes. This paradigm shift enables us to accurately infer causal relationships between transcriptional events.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Review
Cell Biology
Yu Zhao, Ulf Panzer, Stefan Bonn, Christian F. Krebs
Summary: Single-cell biology is rapidly advancing, providing new opportunities for researchers to understand cell signaling and identity in the fields of medicine and biology. Especially for immune-mediated diseases, single-cell studies can delve into the pathogenesis of diseases and potential therapeutic approaches. This review also introduces the latest developments in applying single-cell technologies to understand renal function.
CELL AND TISSUE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Bhavnita Soni, Shailza Singh
Summary: Macrophage phenotype plays a crucial role in the pathogenesis of Leishmanial infection, and the expression levels of cytokine signaling suppressors (SOCS) in correlation with the establishment of infection are not well understood. IL6 induces the expression of SOCS1 and SOCS3 through JAK-STAT signaling, and the immunological crosstalk between TLR2 and IL6 signaling pathways is established during the early stage of Leishmania major infection. Synthetic biology approaches can be used to target the activity of SOCS1 and restore pro-inflammatory cytokine expression during infection.
Article
Biochemical Research Methods
Robin Kretz, Larissa Walter, Nadja Raab, Nikolas Zeh, Ralph Gauges, Kerstin Otte, Simon Fischer, Dieter Stoll
Summary: Most recombinant biotherapeutics used to treat severe illnesses are produced by CHO cells. CHO cells are constantly being developed to improve stability and productivity. Spatial proteomics combined with protein expression data can help identify characteristics of highly efficient recombinant protein-expressing cell lines.
MOLECULAR & CELLULAR PROTEOMICS
(2022)