Article
Plant Sciences
Martin Sak, Ivana Dokupilova, Sarlota Kanukova, Michaela Mrkvova, Daniel Mihalik, Pavol Hauptvogel, Jan Kraic
Summary: In this study, in vitro cell cultures derived from grapevine were induced by different biotic and abiotic elicitors to produce stilbenes. The results showed that cell wall homogenate from F. oxysporum and cellulase from T. viride were more effective in enhancing stilbene production compared to commonly used jasmonates.
Article
Agriculture, Multidisciplinary
Ru Wang, Varun Kumar, Noga Sikron-Persi, Irena Dynkin, David Weiss, Avichai Perl, Aaron Fait, Michal Oren-Shamir
Summary: Elicitation treatments of grape cell cultures with methyl jasmonate, UV-C irradiation, and sucrose induce mild production of stilbenes and flavonoids due to limited substrate availability. However, these treatments cause a synergistic boost of stilbenes production when applied to two phenylalanine-enriched transgenic grape cell lines.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Matthew T. Welling, Myrna A. Deseo, Martin O'Brien, Jacob Clifton, Antony Bacic, Monika S. Doblin
Summary: Cannabis sativa is a versatile plant used for food, fiber, and medicine, producing psychoactive and medicinally important compounds called phytocannabinoids (PCs). In this study, the effects of the phytohormone methyl jasmonate (MeJA) on PC biosynthesis and accumulation in C. sativa were investigated. The results showed a dose-response relationship, with a significant increase in PC content in female inflorescences treated with 15 mM MeJA. Metabolomic analysis and targeted transcript analysis were used to identify key regulatory components contributing to PC production and metabolism. This research enhances our understanding of the oxylipin pathway in C. sativa and provides potential molecular targets for optimizing the medicinal and industrial chemical phenotype.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Food Science & Technology
Nawel Benbouguerra, Ruth Hornedo-Ortega, Francois Garcia, Toni El Khawand, Cedric Saucier, Tristan Richard
Summary: Stilbenes are natural phenolic compounds synthesized by plants in response to stress. They are found in grape berries and wine, with potential anti-obesity effects. Optimized analytical methods are essential for accurate detection and quantification of stilbenes.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Julien Gabaston, Thierry Buffeteau, Agnes Destrac Irvine, Gregory Gambetta, Jean-Michel Merillon, Pierre Waffo-Teguo
Summary: The accurate identification of grapevine cultivars is crucial, and various tools, including E-ε-viniferin, have been developed. The enantiomeric excess of E-ε-viniferin serves as a chemical marker to distinguish grape varieties and establish cultivar relationships through statistical analysis.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Wendell Albuquerque, Parviz Ghezellou, Leif Seidel, Johannes Burkert, Frank Will, Ralf Schweiggert, Bernhard Spengler, Holger Zorn, Martin Gand
Summary: This study presents the first comprehensive identification of proteome content from a white wine (cv. Silvaner). By using mass spectrometry-based proteomics, the wine protein composition was identified through in-solution and in-gel digestion methods after size exclusion chromatographic fractionation. A total of 154 characterized or uncharacterized proteins mainly from Vitis vinifera L. and Saccharomyces cerevisiae were identified, providing valuable information for wine authentication and understanding of organoleptic properties and stability.
Article
Agriculture, Multidisciplinary
Ru Wang, Sangram Keshari Lenka, Varun Kumar, Kelem Gashu, Noga Sikron-Persi, Irena Dynkin, David Weiss, Avichai Perl, Aaron Fait, Michal Oren-Shamir
Summary: The study focuses on enhancing the production of stilbenes, specifically the resveratrol dehydrodimer viniferin, by overexpressing stilbene synthase (STS) and providing ample phenylalanine (Phe). Results show that this strategy significantly increases the accumulation of resveratrol and viniferin in plant cell cultures.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Viviana Martins, Marianne Unlubayir, Antonio Teixeira, Hernani Geros, Arnaud Lanoue
Summary: The study suggests a potential interaction between Ca and MeJA signaling pathways in regulating specific secondary metabolism routes in grape cells. The combination of MeJA+Ca specifically enhances the accumulation of Eresveratrol and E-e-viniferin, while inhibiting the stimulatory effect of MeJA on flavonoid synthesis by down-regulating most flavonoid pathway genes. Ca can mimic or potentiate the effect of MeJA on the expression of JA signaling genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Marisa Maia, Andrea McCann, Cedric Malherbe, Johann Far, Jorge Cunha, Jose Eiras-Dias, Carlos Cordeiro, Gauthier Eppe, Loic Quinton, Andreia Figueiredo, Edwin De Pauw, Marta Sousa Silva
Summary: This study successfully localized the molecules involved in the initial moments of grapevine-Plasmopara viticola interaction using MSI and found their accumulation on the veins. This provides important clues for further understanding the infection mechanism of P. viticola.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Jameel M. Al-Khayri, Roseanne Mascarenhas, Himanshu Madapur Harish, Yashwanth Gowda, Vasantha Veerappa Lakshmaiah, Praveen Nagella, Muneera Qassim Al-Mssallem, Fatima Mohammed Alessa, Mustafa Ibrahim Almaghasla, Adel Abdel-Sabour Rezk
Summary: Stilbenes are polyphenolic allelochemicals synthesized by plants for defense, and they have been used in medicine for their antioxidant, anti-proliferative and anti-inflammatory properties. They modulate inflammatory pathways and reduce the release of cytokines, thereby maintaining homeostasis.
Article
Chemistry, Applied
Milena Stranska, Leos Uttl, Kamila Bechynska, Kamila Hurkova, Adam Behner, Jana Hajslova
Summary: The study demonstrated the potential of metabolomic fingerprinting for characterizing grapevine leaves and canes, with harvesting season explaining most of the variability between samples, followed by different localities and farming systems which also influenced the abundance of metabolites.
Article
Biochemical Research Methods
Sarah Schleicher, Gavin O'Connor, Petra Mischnick
Summary: The methyl distribution of methyl cellulose (MC) is commonly studied by analyzing cello-oligosaccharides (COS) with ESI-MS after perdeuteromethylation. Isotope labeling with (CH3)-C-13 instead of CD3-etherified COS can provide more precise and accurate results. (CH3)-C-13 is more robust in determining the methyl distribution of MCs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Natalia Pudelko-Malik, Jerzy Wisniewski, Dominika Drulis-Fajdasz, Piotr Mlynarz
Summary: A liquid chromatography-mass spectrometry method was developed to quantitatively analyze the presence of a chemical compound in mouse tissues. The study found that the compound is not only present in the liver, heart, and skeletal muscle, but also in different regions of the brain.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Natalia Pudelko-Malik, Jerzy Wisniewski, Dominika Drulis-Fajdasz, Piotr Mlynarz
Summary: A liquid chromatography-mass spectrometry method was developed and validated for the quantitative analysis of a chemical compound in mouse tissues, showing its presence in various brain regions.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Plant Sciences
Lingling Fang, Amit Raj Sharma, Chineche Aniemena, Krystian Roedel, Florence Henry, Philippe Moussou, Anita Samuga, Fabricio Medina-Bolivar
Summary: Stilbene and benzofuran derivatives isolated from white mulberry root have shown various biological activities. Multiple elicitors, including CD, MgCl2, MeJA, and H2O2, were used to enhance the production of these bioactive compounds. The results showed that co-treatment with CD, H2O2, and MgCl2 significantly increased the accumulation of stilbenes and benzofurans in hairy root cultures.
Article
Biochemistry & Molecular Biology
Chiara Cavaliere, Angela Michela Immacolata Montone, Sara Elsa Aita, Rosanna Capparelli, Andrea Cerrato, Paola Cuomo, Aldo Lagana, Carmela Maria Montone, Susy Piovesana, Anna Laura Capriotti
Summary: This paper aimed to comprehensively characterize peptides obtained from soybean protein digestion, with a focus on identifying antioxidant peptides. Two analytical approaches were used to analyze medium-sized and short peptides, leading to the discovery of potential antioxidant amino acid sequences in certain fractions.
Article
Chemistry, Analytical
Sara Elsa Aita, Anna Laura Capriotti, Chiara Cavaliere, Andrea Cerrato, Benedetta Giannelli Moneta, Carmela Maria Montone, Susy Piovesana, Aldo Lagana
Summary: Research on wild neotropical blueberries, endemic to Central and South American regions, has been limited despite their potential as sources of bioactive compounds. This study focused on the phenolic composition of Disterigma alaternoides for the first time, identifying 249 phenolic compounds including anthocyanins, flavonoids, phenolic acids, and proanthocyanidins. The findings highlight the importance of further exploring and understanding the diverse phenolic compounds present in these uncharted matrices.
Article
Biochemical Research Methods
Andrea Cerrato, Susy Piovesana, Sara Elsa Aita, Chiara Cavaliere, Simona Felletti, Aldo Lagana, Carmela Maria Montone, Celia Vargas-de-la-Cruz, Anna Laura Capriotti
Summary: This study investigated the phenolic composition of Vaccinium floribundum Kunth, a species endemic to the Andean regions. High-resolution mass spectrometry analysis revealed that Andean berries are a rich source of phenolic compounds, and the molecular transformations of these compounds during fermentation were comprehensively studied for the first time.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Andrea Cerrato, Sara Elsa Aita, Anna Laura Capriotti, Chiara Cavaliere, Angela Michela Immacolata Montone, Carmela Maria Montone, Aldo Lagana
Summary: In this study, a suspect screening approach based on high-resolution mass spectrometry was used to comprehensively characterize the short endogenous peptidome in dry-cured ham samples. A total of 236 short peptide sequences, including 173 natural short peptides and 63 sequences containing non-proteinogenic amino acids, were tentatively identified. Several modified short peptides, such as pyroglutamination and lactoyl conjugation, were also observed, suggesting reactions occurring over time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Susy Piovesana, Anna Laura Capriotti, Chiara Cavaliere, Andrea Cerrato, Carmela Maria Montone, Riccardo Zenezini Chiozzi, Aldo Lagana
Summary: Site localization of protein sulfation remains challenging despite technological improvements. This study explores different fragmentation strategies and highlights the importance of using metal adducts for sulfation localization.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Organic
Leonardo Straminelli, Francesco Vicentini, Antonio Di Sabato, Carmela Maria Montone, Chiara Cavaliere, Kari Rissanen, Francesca Leonelli, Fabrizio Vetica
Summary: A highly stereoselective procedure for the synthesis of spiro-polycyclic oxindoles bearing five contiguous stereogenic centers including two tetrasubstituted carbons has been developed. The sequence involves sequential organocatalysis using a pyrrolidine-based organocatalyst and DBU, resulting in an atom-economical Michael-domino Michael/aldol reaction sequence. The reaction yielded variously functionalized spiro-decalin oxindoles with excellent stereoselectivity (>99:1 dr, up to 92% ee).
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Andrea Cerrato, Anna Laura Capriotti, Chiara Cavaliere, Carmela Maria Montone, Susy Piovesana, Aldo Lagana
Summary: This paper proposes a method of using 6-azauracil for the nitrogenous Paterno-Buchi reaction of lipids to determine carbon-carbon double bonds in fatty acyl chains using higher collisional dissociation in the negative ion mode. The method improves the ionization efficiency of fatty acid precursors and diagnostic product ions, and also allows for deprotonation of phosphatidylcholines.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Damiano Caputo, Alessandro Coppola, Erica Quagliarini, Riccardo Di Santo, Anna Laura Capriotti, Roberto Cammarata, Aldo Lagana, Massimiliano Papi, Luca Digiacomo, Roberto Coppola, Daniela Pozzi, Giulio Caracciolo
Summary: In this study, a multiplexed strategy combining a nanoparticle-enabled blood test and serum levels of acute-phase proteins demonstrated the ability to distinguish pancreatic cancer patients from healthy controls. The study suggests the potential role of acute-phase proteins as biomarkers for pancreatic cancer and provides a new avenue for the development of multiplexed technologies for early cancer detection.
Review
Plant Sciences
Camilla Badiali, Valerio Petruccelli, Elisa Brasili, Gabriella Pasqua
Summary: Xanthones are valuable secondary metabolites produced by plants with diverse structures and biological activities. This review summarizes the current knowledge of xanthone biosynthesis in plants, including the precursors, enzymes involved, and their cellular and organ localization. The biosynthetic pathway in plants involves the shikimate and acetate pathways, leading to the central intermediate 2,3',4,6-tetrahydroxybenzophenone. Unlike plants, xanthones in fungi and lichens are entirely derived from polyketide. Further studies are needed to investigate the synthesis mechanisms in order to exploit xanthones for practical applications.
Article
Chemistry, Analytical
Andrea Cerrato, Chiara Cavaliere, Carmela Maria Montone, Susy Piovesana
Summary: The paper presents the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides. The material was prepared using specific monomer and cross-linker, and by fine-tuning the polymer properties, it displayed superior performance in terms of the number of enriched glycopeptides and selectivity. These results not only provide a new HILIC material for glycopeptide enrichment but also pave the way for the use of hydrogel materials in this field.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Andrea Cerrato, Sara Elsa Aita, Giuseppe Cannazza, Anna Laura Capriotti, Chiara Cavaliere, Cinzia Citti, Chiara Dal Bosco, Alessandra Gentili, Carmela Maria Montone, Roberta Paris, Aldo Lagana
Summary: Industrial hemp is being widely used for pharmaceutical and nutraceutical purposes, but the detailed composition of hemp remains poorly understood. In this study, the carotenoids and fat-soluble vitamins in the inflorescences of seven Italian industrial hemp varieties were analyzed. The most abundant carotenoids were found to be phytoene, phytofluene, and all-trans -a-carotene. In addition, several other carotenoids were tentatively identified using liquid chromatography coupled with photodiode-array detection.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Carmela Maria Montone, Benedetta Giannelli Moneta, Sara Elsa Aita, Anna Laura Capriotti, Andrea Cerrato, Aldo Lagan, Angela Marchetti, Susy Piovesana, Marianna Villano, Chiara Cavaliere
Summary: The presence of pharmaceuticals in the aquatic environment is mainly due to their release from wastewater treatment plants. Sulfonamides, a class of synthetic antibacterials, have often been found in surface water, contributing to antibiotic resistance. Monitoring their transformation products (TPs) is important, as they may still exert pharmaceutical activity. In this study, three commonly used sulfonamides were incubated with activated sludge, and their TPs were identified using mass spectrometry. The identified TPs were then used to analyze wastewater treatment plant samples. Acetylation was found to be the predominant transformation pathway.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Andrea Cerrato, Alessandra Biancolillo, Giuseppe Cannazza, Chiara Cavaliere, Cinzia Citti, Aldo Lagana, Federico Marini, Massimo Montanari, Carmela Maria Montone, Roberta Paris, Nino Virzi, Anna Laura Capriotti
Summary: This study conducted a comprehensive analysis of industrial hemp samples from different regions in Italy and different cultivars using metabolomics. The study found that cannabinoids, flavonoids, and phenolic acids play a key role in differentiating hemp samples and determining their biological activities.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Guillermo Santos-Sanchez, Ivan Cruz-Chamorro, Carlotta Bollati, Martina Bartolomei, Justo Pedroche, Francisco Millan, Maria del Carmen Millan-Linares, Anna Laura Capriotti, Andrea Cerrato, Aldo Lagana, Anna Arnoldi, Antonio Carrillo-Vico, Carmen Lammi
Summary: This study elucidated the molecular mechanism underlying the hypocholesterolemic effects of Lupin protein hydrolysates (LPHs) in an in vivo model. The results showed that LPH treatment reduced de novo cholesterol synthesis through the regulation of HMGCoAR and LDLR protein levels. LPHs also controlled the PSCK9 signal pathway, leading to decreased cholesterol absorption. These findings highlight LPHs as a potential ingredient for developing new functional foods.
