期刊
NANOTOXICOLOGY
卷 8, 期 -, 页码 118-127出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2013.859321
关键词
Cell membrane; fertility; gold colloids; ligand effect; nanogold; spermatozoa; toxicity
资金
- Masterrind GmbH Verden
- German Research Foundation [SPP1313, BA3580-10-1, RA898-8-.1]
- Excellence Cluster REBIRTH
To examine gold nanoparticle reprotoxicity, bovine spermatozoa were challenged with ligand-free or oligonucleotide-conjugated gold nanoparticles synthesized purely without any surfactants by laser ablation. Sperm motility declined at nanoparticle mass dose of 10 mu g/ml (corresponding to similar to 14 000 nanoparticles per sperm cell) regardless of surface modification. Sperm morphology and viability remained unimpaired at all concentrations. Transmission electron microscopy showed an modification dependant attachment of nanoparticles to the cell membrane of spermatozoa, but provided no evidence for nanoparticle entrance into sperm cells. A molecular examination revealed a reduction of free thiol residues on the cell membrane after nanoparticle exposure, which could explain the decrease in sperm motility. Sperm fertilising ability decreased after exposure to 10 mu g/ml of ligand-free nanoparticles indicating that agglomerated ligand-free nanoparticles interfere with membrane properties necessary for fertilisation. In conclusion, nanoparticles may impair key sperm functions solely by interacting with the sperm surface membrane.
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