Article
Cell & Tissue Engineering
Fabiana Zanata, Lowry Curley, Elizabeth Martin, Annie Bowles, Bruce A. Bunnell, Xiying Wu, Lydia Masako Ferreira, Jeffrey M. Gimble
Summary: This study found similarities between ASC and DF in terms of surface marker expression, differentiation potential, and gene expression, but ASC may have a relatively stronger adipogenesis. However, there is substantial overlap between ASC and DF phenotypes based on biochemical and differentiation metrics.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Plant Sciences
M. H. Hashemi Poor, S. Hosseinzadeh, M. Aminlari
Summary: The study found that PPE can promote proliferation and migration of human dermal fibroblast cells, increase FN1 gene expression, and enhance extracellular matrix components to facilitate wound healing.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Dermatology
Taihao Quan, Yaping Xiang, Yingchun Liu, Zhaoping Qin, Yan Yang, George Bou-Gharios, John J. Voorhees, Andrzej A. Dlugosz, Gary J. Fisher
Summary: The aging process affects the structure and function of dermal collagen, leading to thinning, fragility, wrinkles, laxity, impaired wound healing, and a microenvironment conducive to cancer. CCN1 protein is elevated in aged human skin, and transgenic mice expressing CCN1 in dermal fibroblasts display accelerated skin dermal aging. This mouse model provides a novel tool for understanding and studying the mechanisms of skin aging.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Cell Biology
Xin Shi, Liyuan Jiang, Xin Zhao, Bei Chen, Wei Shi, Yanpeng Cao, Yaowu Chen, Xiying Li, Yusheng He, Chengjie Li, Xiaoren Liu, Xing Li, Hongbin Lu, Can Chen, Jun Liu
Summary: A functional scaffold combining CBD-bFGF and BDDM was developed to enhance stem cell interaction, improve endothelial inducibility, increase stem cell quantity, promote granulation tissue formation and angiogenesis, showing potential application for diabetic wound healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dermatology
Burcu Beksac, Laura Gleason, Sarah Baik, John M. Ringe, Pierluigi Porcu, Neda Nikbakht
Summary: This study investigated the effect of dermal fibroblasts and fibronectin on the survival and proliferation of malignant cutaneous lymphoma cells. The results showed that dermal fibroblasts increased the proliferation rates of the lymphoma cells but did not rescue them from apoptosis. Additionally, an overexpression of a fibronectin isoform was found in the lymphoma skin. The malignant cells expressed fibronectin-binding integrins and adhered to fibronectin, but adhesion-mediated survival was not observed. These findings have important implications for understanding and targeting the malignant skin stromal microenvironment in cutaneous lymphomas.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Ilja L. Kruglikov, Zhuzhen Zhang, Philipp E. Scherer
Summary: Recent data suggest a link between the aging process in dermal fibroblasts and metabolic reprogramming, which involves enhanced fatty acid oxidation and reduced glycolysis. The expansion of dermal white adipose tissue (dWAT) in aged skin covered with hair may trigger this switch, leading to a complex cycle of de-differentiation and re-differentiation in dermal adipocytes. Caveolin-1 and adiponectin play crucial roles in influencing these processes, with potential to manipulate the metabolic preferences of different cell types within the skin microenvironment. Differential expression of adiponectin and caveolin-1 in various cell types may contribute to cellular metabolic heterogeneity within the skin.
Article
Biochemical Research Methods
Stefania Niada, Chiara Giannasi, Cinzia Magagnotti, Annapaola Andolfo, Anna Teresa Brini
Summary: This study utilized quantitative proteomics to explore the protein composition of conditioned medium (CM) and extracellular vesicles (EV) from Adipose-derived Stem/stromal Cells (ASC) and Dermal Fibroblasts (DF). The analysis revealed a distinct protein composition between CM and EV, as well as specific biological processes for the different cell products.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Yoshie Umehara, Miho Takahashi, Hainan Yue, Juan Valentin Trujillo-Paez, Ge Peng, Hai Le Thanh Nguyen, Ko Okumura, Hideoki Ogawa, Francois Niyonsaba
Summary: The skin produces antimicrobial peptides that not only fight against pathogens but also have immunomodulatory functions. This study found that human beta-defensins promote the secretion of angiogenin, a potent angiogenic factor, by fibroblasts, suggesting a novel role of these peptides in angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Eyleen Corrales, Ella Levit-Zerdoun, Patrick Metzger, Silke Kowar, Manching Ku, Tilman Brummer, Melanie Boerries
Summary: Vemurafenib induces paradoxical changes in human dermal fibroblast (HDF) due to a permissive chromatin landscape, leading to compensatory effects during combination therapies with less specific MAPK/ERK inhibitors. The importance of evaluating the effects of drugs on non-transformed stromal components, especially in combination therapies, is highlighted.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Dabin Choi, Wesuk Kang, Soyoon Park, Bomin Son, Taesun Park
Summary: For several decades, excessive glucocorticoids (GCs) have been known to inhibit collagen synthesis and accelerate skin aging, but the molecular mechanisms involved are not well understood. In this study, the researchers identified potential glucocorticoid receptor (GR) target genes that may contribute to the inhibition of collagen synthesis. They confirmed that dexamethasone, a synthetic GC, activates canonical GR signaling in dermal fibroblasts and identified six candidate genes involved in the GC-mediated inhibition of collagen synthesis. This study provides insights into the molecular mechanisms of GC-mediated skin aging and lays the foundation for further research on the biological characteristics of individual GR target genes.
Review
Cell & Tissue Engineering
Frederik Penzien Mamsen, Lea Munthe-Fog, Mikela Karen Mungal Kring, Dominik Duscher, Mikkel Taudorf, Adam J. Katz, Stig-Frederik Trojahn Kolle
Summary: The review highlights the benefits of using scaffolds for ASC delivery, showing improved cell viability, angiogenesis, and wound healing outcomes compared to ASCs alone. The type of scaffold material influences ASC differentiation and therapeutic effects, with natural scaffolds being recommended for fat regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Marta Gerasymchuk, Gregory Ian Robinson, Alyssa Groves, Lucie Haselhorst, Sanjana Nandakumar, Cora Stahl, Olga Kovalchuk, Igor Kovalchuk
Summary: Through research on the effects of pCBs on skin rejuvenation, it was found that THC and CBD can promote cell growth and reduce senescence within a certain concentration range. They also alter nuclear architecture and genes related to cell cycle regulation and ECM production. In addition, compared to other common anti-aging substances, THC and CBD showed better wound healing effects in replicative senescent skin fibroblasts.
Article
Polymer Science
Fong-Sian Lin, Jian- Lee, Alvin Kai-Xing Lee, Chia-Che Ho, Yen-Ting Liu, Ming-You Shie
Summary: Infusing silicon ions into gelatin methacrylate hydrogels can enhance wound healing and skin regeneration by promoting cellular adhesion, proliferation, and secretion of important extracellular matrix proteins. This innovative approach shows promise for future clinical applications in skin regeneration.
Article
Biochemistry & Molecular Biology
Thanh-Diep Ly, Christopher Lindenkamp, Eva Kara, Vanessa Schmidt, Anika Kleine, Bastian Fischer, Doris Hendig, Cornelius Knabbe, Isabel Faust-Hinse
Summary: This study investigates the impact of inflammation on the expression of XYLT1 and XT-I activity, revealing a previously unknown regulatory mechanism involving components of the inflammasome pathway CTSB and caspase-1. These findings contribute to a better understanding of the inflammatory regulation of XT-I.
Article
Biochemistry & Molecular Biology
Maria A. Ahonen, Muhammad Yasir Asghar, Suvi J. Parviainen, Gerhard Liebisch, Marcus Hoering, Marjut Leidenius, Pamela Fischer-Posovszky, Martin Wabitsch, Tomi S. Mikkola, Kid Tornquist, Hanna Savolainen-Peltonen, P. A. Nidhina Haridas, Vesa M. Olkkonen
Summary: miR-221-3p overexpression inhibits terminal differentiation of adipocytes, while its inhibitor increases triglyceride storage. The effects of miR-221-3p may be mediated through 14-3-3 gamma, and it also impacts lipid metabolism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Pharmacology & Pharmacy
Carly Connor, John Hamilton, Lisa Robison, Michael Hadjiargyrou, David Komatsu, Panayotis Thanos
Summary: This study examined the effects of methylphenidate (MP) on the endocannabinoid system. The results showed that abstinence from chronic high dose MP increased CB1 receptor binding, while abstinence from chronic low dose MP decreased CB1 levels.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Neurosciences
Eliz Arnavut, John Hamilton, Rutao Yao, Munawwar Sajjad, Michael Hadjiargyrou, David Komatsu, Panayotis K. Thanos
Summary: This study investigated the effects of chronic use and abstinence of methylphenidate (MP) on rat brain metabolism. The findings suggest that MP dosage and schedule of use may have varying effects on brain metabolism. These results have important implications for understanding the effects of MP on ADHD models, but further research is needed.
Review
Endocrinology & Metabolism
Michael Hadjiargyrou
Summary: The balance between osteoclastic bone resorption and osteoblastic bone formation is crucial for maintaining a healthy skeleton. Osteoporosis, characterized by increased bone resorption and decreased bone formation, leads to weaker bones and a higher risk of fractures. Antiresorptive drugs are commonly used for treatment, but their long-term use may increase the risk of atypical femoral fractures.
Article
Biology
Edwin Dickinson, Melody W. Young, Charles J. Kim, Michael Hadjiargyrou, Michael C. Granatosky
Summary: The ability to securely grasp substrates of variable diameter is critical for arboreal animals. This study examines the pulling and gripping performance of a model parrot species across a range of substrate diameters. The results show that parrots have optimal grip force on small perches, consistent with their preferential perching habits in the wild. Relative to other arboreal specialists, the grasping performance of parrots is comparable, but lower than that of raptorial birds. Further research is needed to understand the adaptive evolutionary context of grasping performance.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Alexander A. Chirokikh, Sardar M. Z. Uddin, Nadine Areikat, Rachel Jones, Edie Duque, Carly Connor, Michael Hadjiargyrou, Panayotis K. Thanos, David E. Komatsu
Summary: Methylphenidate (MP) is commonly used to treat Attention-Deficit/Hyperactivity Disorder (ADHD), but many patients with ADHD also experience depression and anxiety. Therefore, concurrent administration of selective serotonin reuptake inhibitors such as fluoxetine (FLX) is common. This study aimed to investigate the effects of MP and FLX on bone morphology and biomechanical properties in adolescent rats.
Review
Endocrinology & Metabolism
Grace Clouse, Samantha Penman, Michael Hadjiargyrou, David E. Komatsu, Panayotis K. Thanos
Summary: This review examines the role of cannabinoids on osteoporosis, specifically focusing on the endocannabinoid system and its involvement in bone remodeling, as well as the effects of cannabinoids on bone health. Despite many mechanisms still remaining unclear, both preclinical and clinical studies show that the effects of cannabis mediated through the endocannabinoid system may be an effective treatment for osteoporosis.
ARCHIVES OF OSTEOPOROSIS
(2022)
Article
Pharmacology & Pharmacy
Shebin Tharakan, Shams Khondkar, Sally Lee, Serin Ahn, Chris Mathew, Andrei Gresita, Michael Hadjiargyrou, Azhar Ilyas
Summary: In this study, alginate/collagen hydrogels loaded with MC3T3-E1 osteoblasts were 3D printed with Sr-Ca polyphosphate. The results showed that a concentration of 2 mg/mL of Sr-Ca polyphosphate induced the greatest degradation of scaffolds after 7 days and the least swelling after 24 h, while exposure of osteoblasts to 1 mg/mL of Sr-Ca polyphosphate caused severe cytotoxic effects. Overall, low doses of Sr-Ca polyphosphate showed potential as an additive in biomaterials for bone tissue engineering, but the cytotoxic effects due to its pH must be closely monitored.
Article
Biology
Carlyn Annunziata, Haniyeh Fattahpour, Daniel Fong, Michael Hadjiargyrou, Pejman Sanaei
Summary: In vitro tissue engineering scaffolds consist of pores that allow cells to migrate and are supplied with nutrient-rich culture medium. Cell proliferation in elastic scaffolds depends on the deformation of the scaffold pores, which is influenced by the fluid dynamics and nutrient flow rate. This mathematical model and simulation offer insights into the cellular growth within elastic scaffolds.
BULLETIN OF MATHEMATICAL BIOLOGY
(2023)
Review
Geriatrics & Gerontology
Bridget Boston, Deepak Ipe, Bogdan Capitanescu, Andrei Gresita, Stephen Hamlet, Robert Love, Michael Hadjiargyrou, Chien-Ling Huang, Iulian Nusem, Rodica Ileana Miroiu, Aurel Popa-Wagner, Patrick Hans-Heinrich Warnke, Eugen Bogdan Petcu
Summary: Medication-related osteonecrosis of the jaw (MRONJ) refers to the non-healing jawbone ulcerative-necrotic lesion appearing after dental therapy or minor trauma in patients treated previously with certain medications. Studies have been conducted to explore MRONJ's classification, clinical features, and pathophysiology, as well as its management and new trends in treatment. Conservative therapy may not be effective for severe cases of MRONJ, but new methods that promote local angiogenesis and vascularization show promise in limited trials.
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY
(2023)
Article
Orthopedics
Michael Hadjiargyrou, Leonidas Salichos, Peter Kloen
Summary: In this study, the miRNA profiles of normal bone, fracture callus, and nonunion tissues were compared. It was found that certain miRNAs were upregulated or downregulated in normal bone and healing tissues. Target genes and relevant biological processes and signaling pathways were also identified. This study enhances our understanding of the role of miRNAs in fracture repair.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Review
Health Care Sciences & Services
Daniela Senior, Rania Ahmed, Eliz Arnavut, Alexandra Carvalho, Wen Xuan Lee, Kenneth Blum, David E. Komatsu, Michael Hadjiargyrou, Rajendra D. Badgaiyan, Panayotis K. Thanos
Summary: The majority of animal studies on methylphenidate (MP) use methods such as intraperitoneal injections, subcutaneous injections, or the oral gavage route. However, the most clinically relevant route is oral administration. Intraperitoneal injections provide an immediate and maximum dose, but only represent a limited window of the drug's effects. Subcutaneous injections do not accurately reflect oral exposure, and the oral-gavage method has adverse effects and may be stressful for animals. The use of a two-bottle drinking method allows for free consumption of MP and more accurately mirrors human treatment, enabling the study of MP's effects on development, behavior, neurochemistry, and brain function.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Polymer Science
Shebin Tharakan, Iman Raja, Annette Pietraru, Elina Sarecha, Andrei Gresita, Eugen Petcu, Azhar Ilyas, Michael Hadjiargyrou
Summary: Osteosarcoma, a malignant bone tumor, poses significant risks to patient mortality and morbidity. Conventional invasive treatments have drawbacks, prompting researchers to explore the use of hydrogels for targeted therapy. Hydrogels loaded with chemotherapeutic drugs have demonstrated promising results in eradicating tumor cells and promoting bone regeneration. Stimuli-responsive hydrogels can react to the tissue microenvironment, facilitating controlled drug release with modulated biomechanical properties. This narrative review discusses in vitro and in vivo studies on different hydrogels, including stimuli-responsive ones, for the treatment of osteosarcoma, as well as potential future applications for patient treatment.
Article
Physiology
Charles J. Kim, Chanpreet Singh, Christine Lee, Kevin DiMagno, Madison O'Donnell, Marina Kaczmarek, Arhum Ahmed, Jessica Salvo-Schaich, Alexis Perez, William Letsou, Maria-Alicia Carrillo Sepulveda, Raddy L. Ramos, Michael Hadjiargyrou
Summary: Research indicates that Mustn1 is involved in skeletal muscle development and is associated with myogenic differentiation and myofusion. The role of Mustn1 in glucose homeostasis is unclear. By deleting Mustn1 in skeletal muscle, it was found that male mice had lower glycemia and altered gene expression related to glucose metabolism and insulin sensitivity at 2 months of age. However, these differences became insignificant after 4 months. There were no differences observed in female mice. These findings suggest a link between Mustn1 expression and glucose homeostasis during skeletal muscle development.
PHYSIOLOGICAL REPORTS
(2023)
Article
Chemistry, Physical
Andrei Gresita, Iman Raja, Eugen Petcu, Michael Hadjiargyrou
Summary: Successfully reconstructing bone and restoring its dynamic function is a significant challenge. Traditional bone grafting methods carry risks and alternatives are needed. In this study, collagen-coated hyperelastic bone scaffolds were found to support cell adhesion and proliferation, but exhibited reduced structural support after 7 days in culture.
Meeting Abstract
Endocrinology & Metabolism
David Komatsu, Edie Duque, Rachel Jones, Alexander Chirokikh, Sardar Uddin, Layla Tarricone, Panayotis Panayotis K. Thanos, MIchael Hadjiargyrou
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
