期刊
NANOTOXICOLOGY
卷 3, 期 1, 页码 40-45出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/17435390802593057
关键词
C-60-fullerene; hemocytes; innate immune system; lysosomes; mussels; nanoparticles; nanotubes
资金
- Department for Environment, Food and Rural Affairs (Defra, UK) [AE1136]
- Natural Environment Research Council (NERC, UK)
- Nuffield Bursary
- NERC [pml010003] Funding Source: UKRI
- Natural Environment Research Council [pml010003] Funding Source: researchfish
The toxicity and ecotoxicity of nanomaterials is poorly understood and the risks of environmental exposure are largely unknown. In vitro cellular toxicity of C-60-fullerene and carbon nanotubes was investigated by measuring the retention of a cationic probe (neutral red) within the lysosomal compartment of phagocytic blood cells (hemocytes) from marine mussels (Mytilus galloprovincialis). Intra-lysosomal retention of neutral red, over time, is a measure of the stability of the lysosomal membrane and the health of the cell. Aqueous suspensions of carbon nanoparticles (C-60-fullerene - 1.0 and 10 mu g.ml(-1)) induced cytotoxicity in circulating phagocytic hemocytes, which are a key component of the molluscan innate immune system. Hemocytes exposed to the same concentration range of carbon nanotubes were unaffected. These findings are consistent with the hypothesis that C-60-fullerene is cytotoxic on uptake into the endocytic-lysosomal system and that cell injury may be mediated by generation of reactive oxygen species (ROS).
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