期刊
NANOTECHNOLOGY
卷 25, 期 28, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/25/28/285101
关键词
Staphylococcus aureus; poly(styrene-co-maleic acid); lipid nanoparticles; antibiotic resistance; immunoaffinity chromatography
资金
- BBSRC [BB/1005579/1]
- Royal Pharmaceutical Society of Great Britain
- BBSRC [BB/I005579/1, BB/H011005/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H011005/1, BB/I005579/1, 1091726] Funding Source: researchfish
Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of the cell division machinery from the membrane of drug-resistant staphylococcal cells. SMA-lipid nanoparticles solubilized FtsZ-PBP2-PBP2a complexes from intact cells, demonstrating the close physical proximity of these proteins within the lipid bilayer. Exposure of bacteria to (-)-epicatechin gallate, a polyphenolic agent that abolishes beta-lactam resistance in staphylococci, disrupted the association between PBP2 and PBP2a. Thus, SMA purification provides a means to remove native integral membrane protein assemblages with minimal physical disruption and shows promise as a tool for the interrogation of molecular aspects of bacterial membrane protein structure and function.
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