期刊
NANOSCALE
卷 6, 期 12, 页码 6468-6472出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4nr01166j
关键词
-
类别
资金
- MOST [2011CB933201, 2012AA030308, 2012AA022703, 2013AA032204]
- NSFC [51105086, 91213305, 21025520, 51073045]
- Beijing Municipal Science & Technology Commission [Z131100002713024]
In this paper, we present a simple but efficient biomimetic method to encapsulate laccase on mesoporous silica-modified electrospun (ES) ultrafine fibers. Because of the mild immobilization conditions (room temperature, aqueous condition), the encapsulated laccase retained a high activity of 94%. Because of the protection from the silica layer, the laccase worked efficiently at 60 degrees C and retained a long-term activity in the presence of proteinase K. After recycling for 10 times the laccase still preserved 96% of its original reactivity. More remarkably, the immobilized laccase on fibers could completely recover its activity after thermal denature, while the free laccase permanently lost the activity. We also demonstrated that the laccase on silica-coated fibers exhibited an enhanced decolorization capability of Brilliant Blue KN-R (BBKN-R) as compared to the free laccase, showing its great potential for industrial applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据