Article
Chemistry, Multidisciplinary
Christian B. M. Poulie, Emanuel Sporer, Lars Hvass, Jesper T. Jorgensen, Paul J. Kempen, Sara I. Lopes van den Broek, Vladimir Shalgunov, Andreas Kjaer, Andreas Jensen, Matthias M. Herth
Summary: Combining nanotechnology and bioorthogonal chemistry offers the possibility of developing next generation nanomedicines. These nanomedicines can passively target tumors for drug delivery, and bioorthogonal chemistry allows for pretargeting to improve target-specific drug accumulation and induce internalization processes or controlled drug release.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Anthony J. Rojas, Justin M. Wolfe, Heemal H. Dhanjee, Ivan Buslov, Nicholas L. Truex, Richard Y. Liu, Walter Massefski, Bradley L. Pentelute, Stephen L. Buchwald
Summary: The synthesis of palladium oxidative addition complexes derived from unprotected peptides is described. These complexes can react with thiols in aqueous buffer, providing an efficient method for bioconjugation. Using this strategy, peptides can be functionalized with small molecules to prepare modified aryl thioether side-chains at low micromolar concentrations. Additionally, peptide-peptide and peptide-protein ligations are demonstrated under dilute aqueous conditions.
Article
Chemistry, Multidisciplinary
Menghan Cui, Changhui Su, Rong Wang, Qing Yang, Chunxiang Kuang
Summary: This novel and green route involves the direct synthesis of vinyl triazole derivatives with alkynes and triazoles promoted by an inorganic base under transition metal-free conditions, showing great catalytic activity for the anti-Markovnikov stereoselective hydroamination of alkynes. Good yields with excellent functional group tolerance are successfully achieved for a range of substrates, making it an advanced concept for the synthesis of alkenyl triazole with a versatile and cost-efficient approach.
Article
Multidisciplinary Sciences
Xiao-Jing Zhang, Zhuo Zhao, Xia Wang, Min-Hui Su, Lili Ai, Yingying Li, Quan Yuan, Xue-Qiang Wang, Weihong Tan
Summary: A new chemical tailoring strategy was developed to efficiently produce circular bivalent aptamers and cyclized DNA sequences. These modified molecules exhibited high nuclease resistance and significantly improved binding, targeting, and tumor tissue enrichment abilities, thus showing great potential for biomedical applications.
NATIONAL SCIENCE REVIEW
(2023)
Article
Chemistry, Multidisciplinary
Shanmugam Revathi, Aditya L. Shinde, Mulimani K. Rajashekhar, Debasish Mandal, Amit Ranjan Maity, Somenath Garai, Tapas Ghatak
Summary: A novel synthetic approach using innovative metal-free N-heterocyclic imino catalysts has been developed to efficiently synthesize regioselective 1,4-disubstituted 1,2,3-triazoles. Experimental observations have been supported by TD-DFT computational studies. This state-of-the-art metal-free approach serves as an alternative to CuAAC and shows promising potential in the field of synthesizing regioselective 1,4-triazoles.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Olga A. Krasheninina, Julia Thaler, Matthias D. Erlacher, Ronald Micura
Summary: This study introduces a new method for metal-free diazotransfer on native RNA, which can be used for tagging and developing other RNA tools. The azido-RNA obtained by this method can be further processed through well-established bioorthogonal reactions, expanding its applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Applied
Shijun Lin, Shuwen Liu, Guofei Dai, Xiaojin Zhang, Fan Xia, Yu Dai
Summary: This study presents a click-induced fluorescence-quenching sensor based on gold nanoparticles for rapid detection of copper ions and ascorbic acid. The sensor has a fast response time and high sensitivity, able to detect the concentrations of Cu2+ and AA within less than 3 minutes.
Article
Chemistry, Multidisciplinary
Alex Gibney, Raphael E. F. de Paiva, Vandana Singh, Robert Fox, Damien Thompson, Joseph Hennessy, Creina Slator, Christine J. McKenzie, Pegah Johansson, Vickie McKee, Fredrik Westerlund, Andrew Kellett
Summary: In this study, a Cu-catalysed azide-alkyne cycloaddition reaction produced a 1,2,3-triazole linker that was used to create Cu-binding artificial metallo-nucleases (AMNs). A bioactive C-3-symmetric ligand called TC-Thio was developed using biologically inert reaction partners. The ligand formed Cu-II and Cu-I complexes through Cu coordination, and showed potent DNA binding and cleaving abilities. Mechanistic studies revealed that DNA recognition occurred at the minor groove, leading to oxidative damage through a pathway dependent on superoxide and peroxide. Single molecule imaging demonstrated that the complex caused DNA damage recognized by base excision repair enzymes, comparable to the clinical drug temozolomide.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Shuang Zhao, Sha Yang, Hanqing Xu, Xiaoqi Tang, Hongwei Wang, Lianyu Yu, Xiaopei Qiu, Yunxia Wang, Mingxuan Gao, Kai Chang, Ming Chen
Summary: In this study, an enzyme-free fluorescence resonance energy transfer assay (3C-TASK) was developed for the detection of circulating microRNAs (miRNAs). The 3C-TASK utilized click chemical ligation reactions and hairpin stacking circuits to achieve sensitive and specific detection of miRNA. This method offers a new perspective for the broader application of enzyme-free chemical reactions and DNA circuits in biosensing.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Ruijia Huang, Ying Xu, Jihui Du, Qiong Guan, Xiaoqing Cai, Feng Li, Jidong Wang, Wenwen Chen
Summary: Copper is an essential element in the human body and its excessive presence is associated with various diseases. A chemical sensor based on the Cu-catalyzed click reaction and fluorescence-enhanced substrate has been developed for selective detection of Cu2+. This sensor can detect Cu2+ as low as 3.87 nM within 10 minutes and has potential applications in disease diagnosis and antibiotic susceptibility testing.
Article
Immunology
Amelie V. Bos, Martje N. Erkelens, Sebastiaan T. A. Koenders, Mario van der Stelt, Marjolein van Egmond, Reina E. Mebius
Summary: The uptake and metabolism of vitamin A within human immune cells has been difficult to study due to the small, lipophilic nature of retinoids. By synthesizing novel bio-orthogonal retinoid-based probes, researchers were able to monitor retinoid molecules in real-time in immune cells, revealing that various immune cell subsets have the capacity to internalize and metabolize retinoids. This study sheds light on the mechanism of vitamin A metabolism and its importance for human adaptive immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Nisarg Soni, Swarbhanu Sarkar, Abhinav Bhise, Yeong Su Ha, Wonchoul Park, A-Ram Yu, Virendra Kumar, Jeong Eun Lim, Young-Ran Yoon, Jeongsoo Yoo
Summary: The availability of multiple bioorthogonal reactions under physiologically relevant conditions has attracted the interest of biochemists and organic chemists. In this study, the Staudinger ligation reaction was used to release radioactivity from immunoconjugates, improving target-to-background ratios. In vitro and in vivo experiments demonstrated the efficacy of this click cleavage approach in improving tumor-to-blood ratios and enhancing tumor visualization.
Article
Biochemistry & Molecular Biology
Peng Wu
Summary: The Nobel Prize in Chemistry 2022 was awarded to Carolyn R. Bertozzi, Morten Meldal, and K. Barry Sharpless for their contributions to the development of click chemistry and bioorthogonal chemistry. These technologies enable rapid, selective, and nontoxic covalent reactions that link two components together under benign conditions, leading to a paradigm shift in basic research and practical applications.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Bidyutjyoti Dutta, Anirban Garg, Parmita Phukan, Akshay Kulshrestha, Arvind Kumar, Diganta Sarma
Summary: The green protocol utilizing the bifunctional ionic liquid as a catalyst, combined with microwave irradiation for rapid synthesis of 5-amino-1,2,3-triazoles, is a novel approach. The experimental results confirm the low environmental impact and greener attributes of this method.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Nazha Hilali, Waralee Ruankham, Isaac Aaron Morales Frias, Francesca G. Bellagambi, Marie Hangouet, Marie Martin, Joan Bausells, Hasna Mohammadi, Aziz Amine, Nadia Zine, Abdelhamid Errachid
Summary: The aim of this study was to develop an electrochemical biosensor for determining salivary interleukin-10 (IL-10) levels. The use of copper-free click chemistry improved the biofunctionalization strategy and led to the successful development of the sensor. The immunosensor showed a semi-logarithmic linear range from 0.1 pg/mL to 5 pg/mL with an R2 value of 0.9815, a limit of quantitation (LOQ) of 0.1 pg/mL, and a relative standard deviation (RSD) of 10.67%. The specificity of the immunosensor was confirmed, and it was successfully applied to the analysis of saliva samples from a healthy volunteer.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Cheng Jin, Afaf H. Ei-Sagheer, Siqi Li, Katherine A. Vallis, Weihong Tan, Tom Brown
Summary: Oligonucleotides containing cleavable linkers are versatile tools for stimulus-responsive and site-specific DNA cleavage, but their previous limitations have restricted their in vivo applications. Inspired by cathepsin B-sensitive dipeptide linkers in ADCs, we have developed phosphoramidites of Val-Ala-02 and Val-Ala-Chalcone for automated synthesis of enzyme-cleavable oligonucleotides. Cathepsin B efficiently digests these linkers, enabling cleavage of oligonucleotides or release of small-molecule payloads. We believe that these dipeptide linker phosphoramidites will expand the clinical applications of therapeutic oligonucleotides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Maria-Eleni Kyriazi, Afaf H. El-Sagheer, Igor L. Medintz, Tom Brown, Antonios G. Kanaras
Summary: Researchers have overcome the limitation of enzymatic degradation of spherical nucleic acids by introducing a phosphorothioate bond modification on the oligonucleotide backbone, allowing for high selectivity and specificity in the detection of mRNA in cells.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Nicolas Brouckaert, Nina Podoliak, Tetiana Orlova, Denitsa Bankova, Angela F. De Fazio, Antonios G. Kanaras, Ondrej Hovorka, Giampaolo D'Alessandro, Malgosia Kaczmarek
Summary: This study presents a quick and reliable method to characterize colloidal suspensions of liquid crystals doped with nanoparticles using an optical multi-parameter analyzer. The suspensions characterized in this work include plasmonic gold nanoparticles and ferroelectric Sn2P2S6 nanoparticles in liquid crystals. The results show that the elastic constants and rotational viscosity of the liquid crystals exhibit nonlinear dependence on the concentration of the gold nanoparticles, indicating particle aggregation. For the ferroelectric suspension, the elastic constant decreases with doping while the rotational viscosity increases. Additionally, the stability of the ferroelectric suspension over ten years is demonstrated through viscosity measurements.
Article
Multidisciplinary Sciences
Martina F. Ober, Anna Baptist, Lea Wassermann, Amelie Heuer-Jungemann, Bert Nickel
Summary: In this study, silica deposition on DNA origami structures was investigated using in situ small-angle X-ray scattering. The results revealed that silica formation led to condensation and shrinkage of the DNA origami structures. Silicification increased the stability of the structures and provided chemical protection. The study also found that silica not only formed an outer shell, but also penetrated the internal structure of the origami.
NATURE COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Lutfur Rahman, Yasra Sarwar, Shazia Khaliq, Wasim Inayatullah, Wasim Abbas, Ameena Mobeen, Ata Ullah, Syed Zajif Hussain, Waheed S. Khan, Maria-Eleni Kyriazi, Irshad Hussain, Antonios G. Kanaras, Asma Rehman
Summary: In this study, surfactin-coated silver nanoparticles were synthesized and applied for the first time as an efficient antibacterial and antibiofilm agent against drug-resistant bacteria. The nanoparticles showed excellent bactericidal and antibiofilm activity against Pseudomonas aeruginosa, without causing significant dermal irritation or inflammation in vivo. Microscopic analysis also revealed no pathological alterations at the applied concentrations, supporting the potential use of these nanoparticles in combating drug-resistant bacterial biofilms and for skin wound dressings.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Joseph Hennessy, Piotr Klimkowski, Daniel Singleton, Alex Gibney, Malou Coche, Nicholas P. Farrell, Afaf H. El-Sagheer, Tom Brown, Andrew Kellett
Summary: We report a new class of carboplatin-TFO hybrid that incorporates a bifunctional alkyne-amine nucleobase monomer called AP-C3-dT, enabling dual 'click' platinum(ii) drug conjugation and thiazole orange fluorophore coupling. These hybrids exhibit superior stability and crosslinking properties compared to previous Pt(ii)-TFOs, and show sequence-specific binding at neutral pH.
RSC MEDICINAL CHEMISTRY
(2023)
Article
Cell & Tissue Engineering
Elloise Z. Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras, Jonathan West, Ben D. MacArthur, Patrick S. Stumpf, Richard O. C. Oreffo
Summary: Researchers have developed a new method to isolate and enrich skeletal stem cells from human bone marrow, which have the potential to differentiate into bone, cartilage, and fat cells. This study provides valuable insights for further understanding and utilization of these stem cells.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Chemistry, Organic
Chunsen Bai, Piotr Klimkowski, Cheng Jin, Jagannath Kuchlyan, Afaf H. El-Sagheer, Tom Brown
Summary: Oligonucleotides labelled with thiazole orange intercalator and a reporter dye on the same thymine base have been synthesized, enabling dual orthogonal labelling of the nucleobase. These combination probes show high duplex stability and fluorescence when bound to complementary DNA and RNA in their DNA and 2'-OMe RNA formats.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Konstantina Alexaki, Maria Eleni Kyriazi, Joshua Greening, Lapatrada Taemaitree, Afaf H. El-Sagheer, Tom Brown, Xunli Zhang, Otto L. Muskens, Antonios G. Kanaras
Summary: In this study, we successfully demonstrated the rapid and accurate detection of SARS-CoV-2 infection without the need for PCR amplification using UCNPs and GO.
Article
Biochemistry & Molecular Biology
Anna Dysko, Ysobel R. Baker, Graham McClorey, Matthew J. A. Wood, Sabine Fenner, Glynn Williams, Afaf El-Sagheer, Tom Brown
Summary: Oligonucleotides are powerful therapeutics for difficult diseases, but they require chemical modification. Substituting the phosphodiester backbone with a triazole linkage provides stability and improved cell penetration, but reduces RNA target binding. Attaching pyrene or anthraquinone to the ribose sugar of the triazole restores stability and splice switching ability.
RSC CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Laetitia Bardet, Herve Roussel, Stefano Saroglia, Masoud Akbari, David Munoz-Rojas, Carmen Jimenez, Aurore Denneulin, Daniel Bellet
Summary: The thermal instability of silver nanowires leads to increased electrical resistance in AgNW networks. Understanding the relationship between structural and electrical properties of AgNW networks is crucial for their integration as transparent electrodes in flexible optoelectronics. In situ X-ray diffraction measurements were used to study the crystallographic evolution of Ag-specific Bragg peaks during thermal ramping, revealing differences in thermal and structural transitions between bare and SnO2-coated AgNW networks.
Article
Chemistry, Multidisciplinary
Nathalia Cancino-Fuentes, Arnau Manasanch, Joana Covelo, Alex Suarez-Perez, Enrique Fernandez, Stratis Matsoukis, Christoph Guger, Xavi Illa, Anton Guimera-Brunet, Maria V. Sanchez-Vives
Summary: This study provides a comprehensive characterization of graphene-based solution-gated field-effect transistors (gSGFETs) for brain recordings, highlighting their potential clinical applications.
Article
Chemistry, Multidisciplinary
Sikandar Aftab, Hailiang Liu, Dhanasekaran Vikraman, Sajjad Hussain, Jungwon Kang, Abdullah A. Al-Kahtani
Summary: This study examines the effects of hybrid nanoparticles made of NiO@rGO and NiO@CNT on the active layers of polymer solar cells and X-ray photodetectors. The findings show that these hybrid nanoparticles can enhance the charge carrier capacities and exciton dissociation properties of the active layers. Among the tested configurations, the NiO@CNT device demonstrates superior performance in converting sunlight into electricity, and achieves the best sensitivity for X-ray detection.
Article
Chemistry, Multidisciplinary
Hyo Jung Shin, Seung Gyu Choi, Fengrui Qu, Min-Hee Yi, Choong-Hyun Lee, Sang Ryong Kim, Hyeong-Geug Kim, Jaewon Beom, Yoonyoung Yi, Do Kyung Kim, Eun-Hye Joe, Hee-Jung Song, Yonghyun Kim, Dong Woon Kim
Summary: This study investigates the role of SOX9 in reactive astrocytes following ischemic brain damage using a PLGA nanoparticle plasmid delivery system. The results demonstrate that PLGA nanoparticles can reduce ischemia-induced neurological deficits and infarct volume, providing a potential opportunity for stroke treatment.
Article
Chemistry, Multidisciplinary
Anurag Chaudhury, Koushik Debnath, Nikhil R. Jana, Jaydeep K. Basu
Summary: The study investigates the interaction between nanoparticles and cell membranes, and identifies key parameters, including charge, crowding, and membrane fluidity, that determine the adsorbed concentration and unbinding transition of nanoparticles.
Article
Chemistry, Multidisciplinary
Sina Sadeghi, Fazel Bateni, Taekhoon Kim, Dae Yong Son, Jeffrey A. Bennett, Negin Orouji, Venkat S. Punati, Christine Stark, Teagan D. Cerra, Rami Awad, Fernando Delgado-Licona, Jinge Xu, Nikolai Mukhin, Hannah Dickerson, Kristofer G. Reyes, Milad Abolhasani
Summary: In this study, an autonomous approach for the development of lead-free metal halide perovskite nanocrystals is presented, which integrates a modular microfluidic platform with machine learning-assisted synthesis modeling. This approach enables rapid and optimized synthesis of copper-based lead-free nanocrystals.
Article
Chemistry, Multidisciplinary
Zahir Abbas, Nissar Hussain, Surender Kumar, Shaikh M. Mobin
Summary: The rational construction of free-standing and flexible electrodes for electrochemical energy storage devices is an emerging research focus. In this study, a redox-active metal-organic framework (MOF) was prepared on carbon nanofibers using an in situ approach, resulting in a flexible electrode with high redox-active behavior and unique properties such as high flexibility and lightweight. The prepared electrode showed excellent cyclic retention and rate capability in supercapacitor applications. Additionally, it could be used as a freestanding electrode in flexible devices at different bending angles.
Article
Chemistry, Multidisciplinary
Lishan Zhang, Xiaoting Zhang, Hui Ran, Ze Chen, Yicheng Ye, Jiamiao Jiang, Ziwei Hu, Miral Azechi, Fei Peng, Hao Tian, Zhili Xu, Yingfeng Tu
Summary: Photodynamic therapy (PDT) is a promising local treatment modality in cancer therapy, but its therapeutic efficacy is restricted by ineffective delivery of photosensitizers and tumor hypoxia. In this study, a phototactic Chlorella-based near-infrared (NIR) driven green affording-oxygen microrobot system was developed for enhanced PDT. The system exhibited desirable phototaxis and continuous oxygen generation, leading to the inhibition of tumor growth in mice. This study demonstrates the potential of using a light-driven green affording-oxygen microrobot to enhance photodynamic therapy.
Article
Chemistry, Multidisciplinary
Yujin Li, Jing Xu, Xinqi Luo, Futing Wang, Zhong Dong, Ke-Jing Huang, Chengjie Hu, Mengyi Hou, Ren Cai
Summary: In this study, hollow heterostructured materials were constructed using an innovative template-engaged method as cathodes for zinc-ion batteries. The materials exhibited fast Zn2+ transport channels, improved electrical conductivity, and controlled volume expansion during cycling. The designed structure allowed for an admirable reversible capacity and high coulombic efficiency.
Article
Chemistry, Multidisciplinary
Paritosh Mahato, Shashi Shekhar, Rahul Yadav, Saptarshi Mukherjee
Summary: This study comprehensively elucidates the role of the core and electrostatic surface of metal nanoclusters in catalytic reduction reactions. The electrostatic surface dramatically modulates the reactivity of metal nanoclusters.
Article
Chemistry, Multidisciplinary
Pei Liu, Mengdi Liang, Zhengwei Liu, Haiyu Long, Han Cheng, Jiahe Su, Zhongbiao Tan, Xuewen He, Min Sun, Xiangqian Li, Shuai He
Summary: This study demonstrates a simple and environmentally-friendly method for the synthesis of zinc oxide nanozymes (ZnO NZs) using wasted hop extract (WHE). The WHE-ZnO NZs exhibit exceptional peroxidase-like activity and serve as effective catalysts for the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2). In addition, a straightforward colorimetric technique for detecting both H2O2 and glucose was developed using the WHE-ZnO NZs as peroxidase-like catalysts.
Article
Chemistry, Multidisciplinary
Hyunkyu Oh, Young Jun Lee, Eun Ji Kim, Jinseok Park, Hee-Eun Kim, Hyunsoo Lee, Hyunjoo Lee, Bumjoon J. Kim
Summary: Mesoporous carbon particles have unique structural properties that make them suitable as support materials for catalytic applications. This study investigates the impact of channel nanostructures on the catalytic activity of porous carbon particles (PCPs) by fabricating PCPs with controlled channel exposure on the carbon surface. The results show that PCPs with highly open channel nanostructures exhibit significantly higher catalytic activity compared to those with closed channel nanostructures.
Article
Chemistry, Multidisciplinary
Yunjie Lu, Zhaohui Li, Zewei Li, Shihao Zhou, Ning Zhang, Jianming Zhang, Lu Zong
Summary: A tough, long-lasting adhesive and highly conductive nanocomposite hydrogel (PACPH) was fabricated via the synergy of interfacial entanglement and adhesion group densification. PACPH possesses excellent mechanical properties, interfacial adhesion strength, and conductivity, making it a promising material for long-term monitoring of human activities and electrocardiogram signals.
Article
Chemistry, Multidisciplinary
Zichao Wei, Audrey Vandergriff, Chung-Hao Liu, Maham Liaqat, Mu-Ping Nieh, Yu Lei, Jie He
Summary: We have developed a simple method to prepare polymer-grafted plasmonic metal nanoparticles with pH-responsive surface-enhanced Raman scattering. By using pH-responsive polymers as ligands, the aggregation of nanoparticles can be controlled, leading to enhanced SERS. The pH-responsive polymer-grafted nanoparticles show high reproducibility and sensitivity in solution, providing a novel approach for SERS without the need for sample pre-concentration.
Article
Chemistry, Multidisciplinary
Melis Ozge Alas Colak, Ahmet Gungor, Merve Buldu Akturk, Emre Erdem, Rukan Genc
Summary: This research investigates the effect of functionalizing carbon dots with hydroxyl polymers on their performance as electrode materials in a supercapacitor. The results show that the functionalized carbon dots exhibit excellent electrochemical performance and improved stability.