期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 221, 期 -, 页码 427-433出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.06.087
关键词
ECBT; Ratiometric pH probe; Escherichia coli cells; Bioimaging
资金
- National Natural Science Foundation of China [21072119, 21102086]
- Shanxi Province Science Foundation [2013011011-1]
- Natural Science Foundation of Shanxi Province [2006011017]
- Shanxi Province Science Foundation for Youths [2012021009-4]
- Shanxi Province Foundation for Returnee [2012-007]
- Taiyuan Technology star special [12024703]
- Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi [20111001]
- Shanxi Province Foundation for College Students of Science and Technology Innovation projects [2013328]
A ratiometric fluorescence pH probe 2-(N-ethylcarbazole-3-vinyl)-benzothiazole (ECBT) is facilely developed by ethylene bridging of N-ethyl-3-carbazolecarboxaldehyde and benzothiazole, which is a useful fluorescent probe for strongly acidic pH sensing. The pH titration indicates remarkable fluorescence enhancement effect and an emission red-shift of 87nm with pH decreasing from 7.00 to 1.00. Furthermore, the solution color changes from light blue to orange under a UV lamp illumination. A pK(a) value of 1.62 and a good linearity in the pH range of 1.23-2.10 indicate that the probe can be used in quantitative fluorescence imaging under strongly acidic condition. In addition, ECBT exhibits high selectivity and sensitivity, good photostability and excellent reversibility for pH sensing. The probe is further applied successfully to imaging extreme acidity in Escherichia coli cells without influence of autofluorescence in biological systems. 2015 Elsevier B.V. All rights reserved.
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