期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 10, 期 6, 页码 1335-1341出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2014.03.009
关键词
CLEC5A; Dengue virus; Anodic aluminum oxide; Nanostructured biosensor; Electrochemical impedance spectroscopy
资金
- Department of Health of Taiwan [DOH102-TD-N-111-004]
- National Science Council of Taiwan [NSC-101-2212-E-005-022- MY3]
- National Science Council [NSC 101-2325-B-010-006, NSC 101-2321-B-010-003]
- Academia Sinica
- Thematic Research Project [AS-101-TP-B06-2]
- Infection and Immunity Center, National Yang-Ming University, Taiwan (from Ministry of Education, Aim for the Top University Plan)
- Taipei Veterans General Hospital [V101E4-006, V101E4-007, TVGH-NTUH VN-100-06]
- Molecular and Genetic Imaging Core/National Research Program for Genomic Medicine at National Yang-Ming University [NSC99-3112-B-010-015]
In this paper, we develop an effective method for detecting weak molecular bonding between the dengue virus (DV) and its receptor C-type lectin domain family 5, member A (CLEC5A). The CLEC5A-DV interaction is critical for DV-induced hemorrhagic fever and shock syndrome, so the sensing of CLEC5A-DV binding is crucial to realize a thorough study of the pathogenesis of dengue fever. Through a highly sensitive nanostructured sensing electrode of gold nanoparticles (GNPs) uniformly deposited on a nanohemisphere array, a label-free detection of the ultra weak binding between CLEC5A and the DV can be performed with electrochemical impedance spectroscopy (EIS). Experimental results demonstrate that the proposed approach is a highly promising method for investigating weak molecular interactions such as the ligand-receptor interaction of dengue fever, enterovirus (EV), or the interaction between cancer surface glycoproteins and their receptors. (C) 2014 Elsevier Inc. All rights reserved.
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