Review
Cell Biology
Yiqian Luo, Jie Li, Baoqin Li, Yuanliang Xia, Hengyi Wang, Changfeng Fu
Summary: The behavior of nerve cells is crucial in nerve regeneration, with the surrounding mechanical, topographical, and electrical microenvironment playing a key role in influencing nerve cell behavior. Recently, biological scaffolds have been developed with various physical properties to regulate nerve cell behavior conversion, providing a strong driving force for nerve regeneration. Research has focused on the biological basis of mechanical transduction in nerve cells, as well as the impact of physical cues on neurite outgrowth and neural stem cell differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ema Onode, Takuya Uemura, Kiyohito Takamatsu, Takuya Yokoi, Kosuke Shintani, Shunpei Hama, Yusuke Miyashima, Mitsuhiro Okada, Hiroaki Nakamura
Summary: This study found that nerve conduits coated with human induced pluripotent stem cell (hiPSC)-derived neurospheres can promote nerve regeneration and functional recovery in repairing rat sciatic nerve defects. This nerve regeneration approach shows promise as a new clinical therapy for treating peripheral nerve defects.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Xinyi Gu, Xiaoli Chen, Xiaoxuan Tang, Zhihao Zhou, Tingting Huang, Yumin Yang, Jue Ling
Summary: The pure silk fibroin hydrogel with high strength and aligned microgrooved structure showed excellent mechanical properties and good biocompatibility. The aligned microgrooved structure enabled the aligned growth of Schwann cells, promoting axon growth and guiding neurite sprouting. This micropatterned SF hydrogel has great potential for peripheral nerve regeneration.
NANOTECHNOLOGY REVIEWS
(2021)
Article
Ophthalmology
Jiun L. Do, Salam Allahwerdy, Ryan Caezar C. David, Robert N. Weinreb, Mark H. Tuszynski, Derek S. Welsbie
Summary: Human neural stem cells have shown potential in promoting the survival and axonal regeneration of injured retinal ganglion cells, possibly through diffusible factors released by NSCs. Additionally, NSCs integrate with the injured optic nerve and have the potential to establish neuronal relays to restore retinofugal connections.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yu-Fen Chung, Jong-Hang Chen, Ching-Wen Li, Hui-Yu Hsu, Ya-Ping Chen, Chiao-Chan Wang, Ing-Ming Chiu
Summary: Nerve injury is a significant challenge in modern clinics, and understanding the roles of neurotrophic factors in neuro-regeneration is crucial for recovery and treating neurodegenerative diseases. This study shows that combining hIL12p80 with NSCs improves functional recovery and accelerates nerve regeneration in mice with sciatic nerve injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yibin Jia, Guanyi Wang, Yuqing Ye, Enming Kang, Huijun Chen, Zishuo Guo, Xiaosheng He
Summary: TBI is a disease with high morbidity and disability worldwide, despite ongoing neural protection efforts. Due to patient heterogeneity, it is difficult to achieve satisfying results in clinic. Neuroinflammation appears since primary injury occurs, and recent studies suggest NSCs play a central role in neural regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Engineering, Biomedical
Yogambha Ramaswamy, Iman Roohani, Young Jung No, Genevieve Madafiglio, Frank Chang, Furong Zhang, Zufu Lu, Hala Zreiqat
Summary: A microcasting technique was developed to produce non-symmetrical HAp surfaces with nature-inspired topographies, showing potential in promoting osteogenic differentiation. Isolated islands topographies significantly enhanced osteogenic differentiation of stem cells, while honeycomb topographies inhibited proliferation and differentiation.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Mahekta R. Gujar, Yang Gao, Xiang Teng, Wei Yung Ding, Jiaen Lin, Ye Sing Tan, Liang Yuh Chew, Yusuke Toyama, Hongyan Wang
Summary: The ability of stem cells to switch between quiescent and proliferative states is crucial for maintaining tissue homeostasis and regeneration. In this study, researchers found that the microtubule minus-end binding protein Patronin/CAMSAP promotes acentrosomal microtubule growth and quiescent neural stem cell (qNSC) reactivation. This is achieved through its interaction with Arf1 and its involvement in microtubule regulation.
Review
Biotechnology & Applied Microbiology
Yuhui Jiang, Xiaoxuan Tang, Tao Li, Jue Ling, Yumin Yang
Summary: Peripheral nerve injury causes sensory dysfunction and locomotor system degeneration, impacting patients' daily life. Recent advancements in artificial nerve grafts and repair products provide potential strategies for repairing long gapped nerve defects. However, challenges in achieving desirable functional recovery still remain. This review discusses the development of tissue engineering products and bionic grafts for peripheral nerve repair, aiming to improve nerve regeneration and provide solutions for long gaps or thick nerve defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Melissa L. D. Rayner, Adam G. E. Day, Kulraj S. Bhangra, John Sinden, James B. Phillips
Summary: This study provides evidence supporting the effectiveness of using engineered neural tissue (EngNT) as a replacement for nerve autografts, with functional regeneration assessed through histological and electrophysiological outcome measures demonstrating equivalent performance.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Chantal Ripoll, Gaetan Poulen, Robert Chevreau, Nicolas Lonjon, Florence Vachiery-Lahaye, Luc Bauchet, Jean-Philippe Hugnot
Summary: Ependymal cells in the spinal cord play a crucial role in providing a physical barrier and circulating cerebrospinal fluid. They exhibit a dorsal-ventral expression pattern of spinal cord developmental transcription factors and express neurodevelopmental genes throughout human life. The persistence of ependymal cells and their heterogeneity highlight the importance of further investigation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Silvan Klein, Andreas Siegmund, Andreas Eigenberger, Valerie Hartmann, Felix Langewost, Nicolas Hammer, Alexandra Anker, Konstantin Klein, Christian Morsczeck, Lukas Prantl, Oliver Felthaus
Summary: The study investigated three differentiation protocols for differentiating rat ASCs into specialized SC phenotypes, comparing their expressions of neurotrophins and myelin markers. The results showed diverse expression profiles of differentiated ASCs, with different protocols inducing varying levels of expression of neurotrophins and myelin markers.
Article
Multidisciplinary Sciences
Yan Li, Zhenyu Zhong, Cunjing Xu, Xiaodan Wu, Jiaqi Li, Weiyong Tao, Jianglin Wang, Yingying Du, Shengmin Zhang
Summary: This study revealed a new mechanotransductive pathway triggered by 3D micropattern force and its inducing effect on tissue regeneration, providing inspiration for innovative design of biomaterial surfaces. The study investigated the mechanotransduction of human mesenchymal stem cells (MSCs) triggered by 3D micropatterns and its effect on the paracrine of MSCs. The findings showed that 3D micropattern force influenced the spatial reorganization of the cytoskeleton and mediated nucleus alteration, leading to enhanced MSCs paracrine and tissue regeneration.
NATIONAL SCIENCE REVIEW
(2023)
Review
Medicine, Research & Experimental
Shyamaladevi Babu, Madhan Krishnan, Arjunkumar Panneerselvam, Mayilvanan Chinnaiyan
Summary: Each year, thousands of people suffer from traumatic peripheral nerve lesions, which can lead to mobility and sensibility impairment and even fatal outcomes. Peripheral nerve regeneration through cell therapy has become a cutting-edge technique. This review focuses on the properties of different types of mesenchymal stem cells (MSCs) that are crucial for peripheral nerve regeneration after nerve injury. ADSCs are considered the most relevant and promising MSCs for repairing peripheral nerve lesions, due to their ability to promote axonal growth, excellent paracrine activity, potential for differentiation, low immunogenicity, and high post-transplant survival rate.
Article
Neurosciences
Ziying Zhu, Xiaona Zhang, Haojie Hao, Heran Xu, Jun Shu, Qian Hou, Min Wang
Summary: Exosomes derived from umbilical cord mesenchymal stem cells (UC-MSCs) play a key role in promoting wound repair and skin regeneration by recruiting and stimulating fibroblasts to secrete nerve growth factors (NGFs) for skin nerve repair.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Amit Nain, Shih-Chun Wei, Yu-Feng Lin, Yu-Ting Tseng, Ranju Prasad Mandal, Yu-Fen Huang, Chih-Ching Huang, Fan-Gang Tseng, Huan-Tsung Chang
Summary: BSA-encapsulated copper sulfide nanocrystals (CuS NCs) were prepared and exhibited enzyme-like and photodynamic activities. under NIR laser irradiation, the antimicrobial response was significantly enhanced and >99% of bacteria were eradicated in just 1 min. The BSA-CuS NCs showed insignificant cytotoxicity and hemolysis, making them highly biocompatible bactericides for effective bacteria eradication in preclinical applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Wei-Jen Chan, Han-Lin Cho, Venkatesh Goudar, Sasinan Bupphathong, Chi-Hung Shu, Chi Kung, Fan-Gang Tseng
Summary: PVA/BA NPs showed high boron-loading capacity, preferential accumulation in brain and oral cancer cells, and lower cytotoxicity towards these cells compared to fibroblasts and macrophages. The results suggest that PVA/BA NPs could potentially serve as an efficient delivery system for BNCT with improved therapeutic outcomes.
Article
Chemistry, Multidisciplinary
Mukta Sharma, Venkanagouda S. Goudar, Manohar Prasad Koduri, Fan Gang Tseng, Mahua Bhattacharya
Summary: The study introduces a novel region-estimation algorithm using quantification of colon-cancer and fibroblast cells to estimate the densest region of colon-cancer cells in 3D co-cultured spheroids. The proposed methodology outperformed existing approaches in terms of segmentation, quantification, and reducing biasing error.
APPLIED SCIENCES-BASEL
(2021)
Article
Cell Biology
Hsueh-Yao Chu, Chih-Yung Yang, Ping-Hao Yeh, Chun-Jieh Hsu, Lu-Wei Chang, Wei-Jen Chan, Chien-Ping Lin, You-You Lyu, Wei-Cheng Wu, Chun-Wei Lee, Jen-Kuei Wu, Jeng-Kai Jiang, Fan-Gang Tseng
Summary: CTCs can be used for predicting recurrence in CRC patients. CTC clusters/microemboli have higher correlation with metastasis, and when both the number of CTCs and serum CEA levels are high, the prognosis in CRC patients may be worse.
Article
Nanoscience & Nanotechnology
Venkanagouda S. Goudar, Manohar Prasad Koduri, Yen-Nhi Ngoc Ta, Yunching Chen, Li-An Chu, Long-Sheng Lu, Fan-Gang Tseng
Summary: Three-dimensional spheroid culture provides a closer model of tumor growth in its natural context with the presence of the collagen network still unclear. A 3D co-culture model was developed to mimic the tumor microenvironment, showing increased drug resistance in tumor spheroids compared to 2D culture. Targeting collagen homeostasis can sensitize the spheroids to chemotherapy drugs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Kuan-Hung Chen, Shih-Han Lee, Li-Ching Kok, Tseren-Onolt Ishdorj, Hwan-You Chang, Fan-Gang Tseng
Summary: The study introduces a novel multi-functional microfluidic system, 3D-ACEK/SERS, which can rapidly concentrate bacteria from whole blood, identify bacterial species, and determine antibiotic susceptibilities. This system utilizes a hybrid electrokinetic mechanism to achieve high concentration efficiency and quick detection, with potential clinical applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Medicine, General & Internal
Yun-Jie Hao, Chih-Yung Yang, Ming-Hsien Chen, Lu-Wey Chang, Chien-Ping Lin, Liang-Chuan Lo, Sheng-Chieh Huang, You-You Lyu, Jeng-Kai Jiang, Fan-Gang Tseng
Summary: Insufficient prognosis of local recurrence in colorectal cancer (CRC) patients contributes to poor progression-free survival rate and death. This study investigated the potential of plasma/exosomal microRNA-21 (miR-21) expressions in predicting CRC recurrence and the possibility of improving prognostic efficacy in early CRC recurrence by combining biomarkers. The results showed significant correlations between plasma and exosomal miR-21 expressions, as well as similar correlations with other biomarkers. Different values of predicting CRC recurrence were observed in stratified patients based on high levels of exosomal miR-21 and plasma miR-21. Furthermore, the use of circulating tumour cells/microemboli (CTCs/CTM) as sensitive biomarkers showed comparable or even better performances in predicting CRC recurrence in late-stage patients. Therefore, analyzing combined biomarkers could improve the efficacy of prognosing CRC recurrence and differentiate recurrence in stratified patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Aum Sagar Panda, Yi-Chien Lee, Chen-Jung Hung, Kang-Ping Liu, Cheng-Yen Chang, Gkreti-Maria Manesi, Apostolos Avgeropoulos, Fan-Gang Tseng, Fu-Rong Chen, Rong-Ming Ho
Summary: This study demonstrates a facile method for the controlled orientation of nanostructures in thin films using a diblock copolymer system. By manipulating the pressure and surface tension of the polymer materials, the researchers were able to achieve long-range order in the nanostructures.
Article
Chemistry, Analytical
Kirsty Muldoon, Zeeshan Ahmad, Yu-Chuan Su, Fan-Gang Tseng, Xing Chen, James A. D. McLaughlin, Ming-Wei Chang
Summary: Personalised drug delivery systems with real-time imaging have the potential to revolutionize diagnostic and therapeutic applications. In this study, a 3D-printed drug delivery matrix with real-time imaging capability was developed, offering promising applications in drug delivery systems, medical imaging, and targeted therapy.
Editorial Material
Cell Biology
Tuhin Subhra Santra, Fan-Gang Tseng
Article
Biotechnology & Applied Microbiology
Wei-Jen Chan, Sasinan Bupphathong, Han-Lin Cho, Venkanagouda S. Goudar, Sina Dehestani, Chi-Shiun Chiang, Fan-Gang Tseng
Summary: Poly(vinyl alcohol)/boric acid crosslinked nanoparticles (PVA/BA NPs) demonstrated effective therapeutic results for oral cancer treatments in BNCT, with a significant reduction in tumor size compared with clinically used boronophenylalanine in a murine model.
Article
Polymer Science
Fu-Rong Chen, Rong-Ming Ho, Chen-Jung Hung, Aum Sagar Panda, Yi-Chien Lee, Shih-Yi Liu, Jheng-Wei Lin, Hsiao-Fang Wang, Apostolos Avgeropoulos, Fan-Gang Tseng
Summary: In this study, the controlled self-assembly of star-block polystyrene-blockpolydimethylsiloxane (PS-b-PDMS) thin films was directly visualized using in situ transmission electron microscopy (TEM) observations. The self-alignment process of film-spanning perpendicular cylinders in the block copolymer (BCP) thin films was investigated under low-dose conditions using an environmental chip with a built-in microheater. The time-resolved self-alignment process was systematically compared between symmetric and asymmetric conditions, providing comprehensive insights into the nucleation and growth mechanism.
Article
Chemistry, Analytical
Fan-Gang Tseng, Wei-Cheng Chiu, Po-Jung Huang
Summary: This paper proposes a Swiss-roll-type mini-reformer employing a copper-zinc catalyst for high-efficient SRM process. The Swiss-roll-shaped design improves thermal energy preservation and temperature uniformity, resulting in higher conversion rates and better long-term durability compared to other geometries.
Article
Chemistry, Analytical
Ashish Kumar, Venkanagouda S. Goudar, Kiran Kaladharan, Tuhin Subhra Santra, Fan-Gang Tseng
Summary: Temperature dynamically regulates the reactivity of biomolecules in the cellular environment, including solid tumors. This study utilized fluorescent polymeric nano-thermometers (FPNTs) to measure the intratumor temperature in co-cultured 3D tumor spheroids. The results showed that FPNTs have great stability, biocompatibility, and high intensity in a biological medium. They can be used as a multifunctional adjuvant to examine thermoregulation in tumor spheroids and provide physiologically relevant spatio-temporal information about solid tumors.
Article
Chemistry, Analytical
Pallavi Gupta, Srabani Kar, Ashish Kumar, Fan-Gang Tseng, Shantanu Pradhan, Pallab Sinha Mahapatra, Tuhin Subhra Santra
Summary: Optoporation-based delivery technique using nano-spiked core-shell gold-coated polystyrene nanoparticles (ns-AuNPs) enables high-throughput intracellular delivery of model cargo within 3D cancer spheroids, demonstrating potential translational benefits for anti-cancer therapeutics to tumors by achieving high delivery efficiency with minimal cellular damage.
Article
Nanoscience & Nanotechnology
Daniela Rubanova, Svitlana Skoroplyas, Alena Libanska, Eva Randarova, Josef Bryja, Michaela Chorvatova, Tomas Etrych, Lukas Kubala
Summary: This study explored the use of nanocarriers to deliver dexamethasone for treating rheumatoid arthritis. The results showed that this method increased the accumulation of dexamethasone in the arthritic area, had a dose-dependent healing effect, and reduced the accumulation of the RA mediator RANKL. This study provides a feasible strategy for improving rheumatoid arthritis therapy.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Article
Nanoscience & Nanotechnology
Thanpisit Lomphithak, Apiwit Sae-Fung, Simone Sprio, Anna Tampieri, Siriporn Jitkaew, Bengt Fadeel
Summary: In this study, iron-doped hydroxyapatite (FeHA) was evaluated as a potential nanomedicine approach for treating PDAC. The results showed that FeHA, in combination with a sublethal dose of the GPX4 inhibitor RSL3, could trigger ferroptosis in KRAS mutant PANC-1 cells while sparing normal human cells. The induction of ferroptosis by FeHA plus RSL3 was found to be dependent on the metalloreductase STEAP3 in PDAC cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Review
Nanoscience & Nanotechnology
Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patino Marin, Miguel Angel Casillas Santana, Horacio Bach
Summary: The emergence of antibiotic-resistant bacteria in severe infections is a growing concern, particularly in hospital environments. Metal-based nanomaterials have shown potential as an alternative to combat these bacteria, but there is a risk of bacterial resistance and environmental accumulation.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)