期刊
NANOMEDICINE
卷 8, 期 12, 页码 1913-1925出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm.12.209
关键词
brain tumor model; convection-enhanced delivery; drug delivery system; immunoliposome; in vivo imaging; nanomedicine; quantum dot; targeted cancer therapy; targeted nanoparticle
资金
- National Cancer Institute [NIH P50 CA 58207-01, NIH P50 CA CA097257, NIH U54 CA90788]
- Delores R Malone American Brain Tumor Association
- National Cancer Institute Cancer Center
- DOD BCRP [BC045345]
- Agilent Technologies Foundation [NA270, 09-US-270]
Aim: The aim of this work is to evaluate combining targeting strategy and convection-enhanced delivery in brain tumor models by imaging quantum dot-immunoliposome hybrid nanoparticles. Materials & methods: An EGF receptor-targeted, quantum dot-immunoliposome hybrid nanoparticle (QD-IL) was synthesized. In vitro uptake was measured by flow cytometry and intracellular localization was imaged by confocal microscopy. In the in vivo study, QD-ILs were delivered to intracranial xenografts via convection-enhanced delivery and fluorescence was monitored noninvasively in real-time. Results: QD-ILs exhibited specific and efficient uptake in vitro and exhibited approximately 1.3- to 5.0-fold higher total fluorescence compared with nontargeted counterpart in intracranial brain tumor xenografts in vivo. Conclusion: QD-ILs serve as an effective imaging agent in vitro and in vivo, and the data suggest that ligand-directed liposomal nanoparticles in conjunction with convection-enhanced delivery may offer therapeutic benefits for glioblastoma treatment as a result of specific and efficient uptake by malignant cells.
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