Article
Chemistry, Applied
Manzar Alam, Sabeeha Ali, Ghulam Md Ashraf, Anwar L. Bilgrami, Dharmendra Kumar Yadav, Md Imtaiyaz Hassan
Summary: Epigallocatechin 3-gallate (EGCG), a polyphenolic component derived from green tea extract, possesses various biological functions, including anti-cancer and anti-inflammatory properties. It modulates multiple signaling pathways to inhibit tumor growth and induce cell death. EGCG also exhibits antioxidant, anti-angiogenic, and apoptotic effects. Therefore, EGCG shows potential in cancer treatment.
Review
Pharmacology & Pharmacy
Sze Wan Hung, Yiran Li, Xiaoyan Chen, Kai On Chu, Yiwei Zhao, Yingyu Liu, Xi Guo, Gene Chi-Wai Man, Chi Chiu Wang
Summary: Green tea, with its main component EGCG, shows potential in suppressing reproductive cancers by regulating autophagy and other cellular processes.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Priscila Baltazar Goncalves, Ana Carolina Renno Sodero, Yraima Cordeiro
Summary: The major bioactive compound EGCG in green tea shows potential for treating neurodegenerative diseases by targeting protein misfolding and aggregation. There is an urgent need to develop effective drugs for these incurable ailments, and EGCG may offer opportunities in drug discovery for NDs.
Article
Oncology
Feng Li, Syeda Qasim, Dapeng Li, Q. Ping Dou
Summary: In-depth insights on cancer biology have highlighted the important roles of epigenetic mechanisms in tumorigenesis. Targeting the cancer epigenome with epi-drugs, including phytochemicals like EGCG, offers promising strategies for cancer chemoprevention and therapy. However, there are limitations to using EGCG as an epigenetic modulator in cancer treatment.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Multidisciplinary Sciences
John M. Starbuck, Sergi Llambrich, Ruben Gonzalez, Julia Albaiges, Anna Sarle, Jens Wouters, Alejandro Gonzalez, Xavier Sevillano, James Sharpe, Rafael De La Torre, Mara Dierssen, Greetje Vande Velde, Neus Martinez-Abadias
Summary: The study found that in the DS mouse model, low doses of GTE-EGCG were beneficial for improving facial skeletal morphology, while early supplementation of EGCG in DS children was associated with reduced facial dysmorphology. However, high doses of GTE-EGCG usage led to increased facial dysmorphology, indicating dose-dependent effects of GTE-EGCG on modulating facial development.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Victor Valverde-Salazar, Daniel Ruiz-Gabarre, Vega Garcia-Escudero
Summary: Alzheimer's disease (AD) is a common form of dementia characterized by memory decline, cognitive impairment, and several pathological changes in the brain. Oxidative stress and inflammation play important roles in the development of AD. Green tea and its active compound EGCG have shown potential therapeutic effects in modulating AD through their antioxidative and anti-inflammatory properties. EGCG can regulate inflammatory processes, reduce protein aggregation, and promote neuronal survival pathways, making it a suitable candidate for the treatment of neurodegenerative disorders involving oxidative stress and inflammation.
Article
Pharmacology & Pharmacy
Changchao Huan, Weiyin Xu, Bo Ni, Tingting Guo, Haochun Pan, Luyao Jiang, Lin Li, Jingting Yao, Song Gao
Summary: The natural molecule EGCG, found in green tea, is effective against PEDV infection at the cellular level by inhibiting attachment, entry, replication, and assembly processes of the virus.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Juliana Hofstatter Azambuja, Rubia Isler Mancuso, Fernanda Isabel Della Via, Cristiane Okuda Torello, Sara Teresinha Olalla Saad
Summary: The study found that green tea extract and epigallocatechin-3-gallate (EGCG) can effectively inhibit the inflammatory response induced by lipopolysaccharide, regulate the inflammatory phenotype of macrophages, reduce M1-macrophages, increase Treg cells, increase levels of M2-macrophages, N2-neutrophils, and Treg cells, and block the migration of monocytes, thus reducing inflammation damage.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Review
Neurosciences
Luiza Fernandes, Thyago R. Cardim-Pires, Debora Foguel, Fernando L. Palhano
Summary: The accumulation of protein aggregates in human tissues is a hallmark of more than 40 diseases called amyloidoses, with seven of them being associated with neurodegenerative processes. EGCG, a natural compound found in green tea, has shown potential in inhibiting protein aggregation, reducing toxicity, and remodeling fibril structures in neurodegenerative diseases such as AD, PD, and HD.
FRONTIERS IN NEUROSCIENCE
(2021)
Review
Chemistry, Analytical
Iyyakkannu Sivanesan, Manikandan Muthu, Anusha Kannan, Suraj Shiv Charan Pushparaj, Jae-Wook Oh, Judy Gopal
Summary: This review discusses the analytical techniques for the key component Epigallocatechin-3-gallate (EGCG) in green tea and surveys its application in in vitro, in vivo, and biological sample analysis. The importance of exploring matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in this field is emphasized.
Article
Biochemistry & Molecular Biology
Anshu Agarwal, Vikash Kansal, Humaira Farooqi, Ram Prasad, Vijay Kumar Singh
Summary: Head and neck cancers, particularly head and neck squamous cell carcinoma (HNSCC), are highly lethal and their prognosis is poor. Epigenetic alterations, such as DNA hypermethylation, play a crucial role in HNSCC development. This study investigated the effect of EGCG, a compound found in green tea, on DNA hypermethylation and HNSCC growth. The results showed that EGCG significantly inhibited DNA hypermethylation and reduced the activity and expression of DNA methyltransferases, leading to the reactivation of tumor suppressor genes and decreased cell proliferation in HNSCC cells. In an in vivo study, EGCG supplementation effectively inhibited tumor growth in mice xenografts. Overall, this study highlights the potential of EGCG as a promising therapeutic strategy for HNSCC.
Article
Food Science & Technology
Klaus W. Lange, Katharina M. Lange, Yukiko Nakamura
Summary: Alzheimer's disease is a global public health challenge with no effective treatment available. Research suggests that bioactive components in green tea may have preventive effects against AD, but clinical evidence is insufficient to support this claim.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Review
Food Science & Technology
Chunpeng Wan, Jian Ouyang, Mingxi Li, Kannan R. R. Rengasamy, Zhonghua Liu
Summary: Diabetes mellitus is a major non-communicable disease with high mortality rates and economic burden. Green tea extract (GTE) and epigallocatechin-3-O-gallate (EGCG) found in green tea have shown promising results in managing diabetes by acting as potent antioxidants. The beneficial effects of green tea and its catechins, particularly EGCG, make it an attractive pharmacological agent for treating diabetes and its complications.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Review
Biochemistry & Molecular Biology
Daniela Mokra, Jana Adamcakova, Juraj Mokry
Summary: This article reviews the biological effects of (-)-Epigallocatechin-3-gallate (EGCG) in respiratory diseases, including antioxidant, anti-inflammatory, anti-fibrotic, pro-apoptotic, anti-tumorous, and metabolic effects. EGCG may be beneficial in respiratory diseases through modulation of various intracellular signaling cascades.
Article
Agriculture, Multidisciplinary
Jingxin Chen, Wanyao Ma, Jiangchuan Yu, Xi Wang, Hongling Qian, Ping Li, Haiqiong Ye, Yue Han, Zhengding Su, Meng Gao, Yongqi Huang
Summary: This study found that the green tea polyphenol EGCG promotes the formation of liquid tau droplets by interacting with the proline-rich domain of tau through hydrophobic interactions and hydrogen bonds. The oxidation of EGCG, tau phosphorylation, and the chemical structure of polyphenols all influence the effectiveness of EGCG in facilitating tau liquid-liquid phase separation (LLPS). These findings provide new insights into the biological activity of EGCG and suggest potential mechanisms for its role in alleviating neurodegenerative diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Cell Biology
Lisa Sophie Kolln, Omar Salem, Jessica Valli, Carsten Gram Hansen, Gail McConnell
Summary: This study presents a new method to reduce nonspecific signals in immunofluorescence images using a convolutional neural network. The network is trained to increase the image contrast of target structures and further improves the image quality with the use of a multiscale structural similarity loss function.
JOURNAL OF CELL SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Jitkasem Meewan, Sukrut Somani, Partha Laskar, Craig Irving, Margaret Mullin, Stuart Woods, Craig W. Roberts, Abdullah R. Alzahrani, Valerie A. Ferro, Suzanne McGill, Stefan Weidt, Richard Burchmore, Christine Dufes
Summary: The protein corona formed on the nanoparticle surface has a strong influence on cell interactions and uptake of nanomedicines. This study shows that the protein corona affects the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. PEGylation gives stealth effects to the zein micelles and makes them promising drug delivery systems for cancer therapy.
Article
Pharmacology & Pharmacy
Christopher F. van der Walle, Christine Dufes, Arpan S. Desai, Julie Kerby, Joanne Broadhead, Alice Tam, Zahra Rattray
Summary: With the establishment of the UK Academy of Pharmaceutical Sciences Advanced Therapy Medicinal Products Focus Group, a webinar series on current and emerging trends in cell and gene therapy was held, discussing the challenges and opportunities in this field and making a significant impact on the global pharmaceutical sciences research landscape.
Article
Multidisciplinary Sciences
Xue Yan Ho, Sean Coakley, Rumelo Amor, Victor Anggono, Massimo A. Hilliard
Summary: This study uncovers the key role of metalloprotease ADM-4 in axonal fusion, providing a molecular target for axonal repair.
Article
Nanoscience & Nanotechnology
Jitkasem Meewan, Sukrut Somani, Jamal Almowalad, Partha Laskar, Margaret Mullin, Graeme MacKenzie, Swapnil Khadke, Yvonne Perrie, Christine Dufes
Summary: This study evaluated the production of zein nanoparticles using a microfluidic system and found that adjusting the PEG density and chain length improved the stability and drug uptake of the nanoparticles.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Immunology
Eliana Battistella, Juan F. Quintana, Gail McConnell
Summary: In this study, a newly-developed light-sheet illuminator and Mesolens objective lens were used to successfully image the level of neuroinflammation in chronically infected mice and compare it to naive controls. Morphometric analyses and spatial distribution of GFAP(+) cells revealed an increase in cell dendrite branching caused by the infection. This is the first report to utilize light-sheet mesoscopy to study the cellular changes in the mouse brain during inflammatory responses to parasitic infection in intact cleared organs, opening up new avenues for studying host-pathogen interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Eliana Battistella, Jan Schniete, Katrina Wesencraft, Juan F. Quintana, Gail McConnell
Summary: By using light-sheet mesoscopy, we have developed a high-speed imaging technique that allows rapid imaging of large biological tissue specimens. This opens up new possibilities for high-content, higher-throughput optical bioimaging at the mesoscale.
Article
Microscopy
Liam M. Rooney, Beatrice Bottura, Katherine Baxter, William B. Amos, Paul A. Hoskisson, Gail McConnell
Summary: This article provides an overview of the development and application of the Mesolens in microbiology. The Mesolens is an ideal imaging method for studying large microbial specimens with microscopic detail due to its unique combination of low magnification and high numerical aperture. The current applications of the Mesolens in microbial imaging are demonstrated, and the potential for impacting other key areas of microbiology is outlined.
JOURNAL OF MICROSCOPY
(2023)
Article
Optics
Peter W. Tinning, Jana K. Schniete, Ross Scrimgeour, Lisa S. Kolln, Liam M. Rooney, Trevor J. Bushell, Gail Mcconnell
Summary: Multi-wavelength standing wave microscopy and interference reflection microscopy are powerful techniques for studying topographical structures using optical interference. However, imaging complex cell surfaces with more than two wavelengths results in complicated topographical maps and difficulties in resolving three-dimensional contours. This study presents a simple image processing method to reduce the complexity of multi-wavelength interference microscopy and produce clearer and more precise topographical maps of cellular structures.
Article
Physics, Applied
S. Foylan, W. B. Amos, J. Dempster, L. Kolln, C. G. Hansen, M. Shaw, G. McConnell
Summary: Total internal reflection fluorescence (TIRF) illumination utilizes an evanescent field to excite fluorophores close to a sample substrate, bypassing the axial diffraction limit of light. However, the standard TIRF imaging requires a high numerical aperture (NA), which limits the field of view. We have developed a prism-based TIRF illuminator for the Mesolens, which has a significantly larger field of view and higher optical throughput compared to traditional TIRF objectives.
APPLIED PHYSICS LETTERS
(2023)
Article
Microscopy
Shannan Foylan, Jana Katharina Schniete, Lisa Sophie Kolln, John Dempster, Carsten Gram Hansen, Michael Shaw, Trevor John Bushell, Gail McConnell
Summary: Standing wave (SW) microscopy is a method that uses interference pattern to excite fluorescence from labelled cellular structures and produce high-resolution images. Here, we report upscaling of this method from microscale to mesoscale using Mesolens, which has low-magnification and high-numerical aperture. With this method, we can produce SW images within a field of view of 4.4 mm x 3.0 mm that can accommodate over 16,000 cells, and apply it to study cells under flow conditions.
JOURNAL OF MICROSCOPY
(2023)
Review
Neurosciences
Hamid Hasani, Jipeng Sun, Shuyu I. Zhu, Qiangzhou Rong, Florian Willomitzer, Rumelo Amor, Gail McConnell, Oliver Cossairt, Geoffrey J. Goodhill
Summary: One of the holy grails of neuroscience is to record the activity of every neuron in the mammalian brain while the animal is freely moving and performing behavioral tasks. Recent progress has been made in large-scale neural recording in rodent models, but achieving single neuron resolution across the entire mammalian brain remains challenging. However, the larval zebrafish, with its transparency and genetic indicators, offers great potential for whole-brain recordings at single-neuron resolution. Recent advances, particularly in light-field microscopy techniques, have enabled brain imaging of immobilized zebrafish.
FRONTIERS IN NEUROSCIENCE
(2023)
Editorial Material
Physics, Applied
S. Foylan, W. B. Amos, J. Dempster, L. Kolln, C. G. Hansen, M. Shaw, G. McConnell
APPLIED PHYSICS LETTERS
(2023)
Article
Pharmacology & Pharmacy
Khadeejah Maeyouf, Intouch Sakpakdeejaroen, Sukrut Somani, Jitkasem Meewan, Hawraa Ali-Jerman, Partha Laskar, Margaret Mullin, Graeme Mackenzie, Rothwelle J. Tate, Christine Dufes
Summary: This study explores the potential of novel hybrid lipid nanoparticles for treating prostate cancer by improving the delivery of therapeutic DNA and drugs. The nanoparticles efficiently entrapped docetaxel and demonstrated proficient DNA condensation, leading to increased uptake by cancer cells and enhanced anti-proliferative efficacy and gene expression levels.
Article
Microbiology
Beatrice Bottura, Liam M. Rooney, Paul A. Hoskisson, Gail McConnell
Summary: The morphology of nutrient-transporting channels in mature E. coli biofilms is affected by growth substrate composition and nutrient availability. The channels exhibit non-linear increase in width with radial distance from the biofilm center. Channel density and width differ between colonies grown on rich and minimal media. This research provides new insights into infection and pathogenicity.
