Review
Medicine, General & Internal
Cristina Irigoyen, Asier Amenabar Alonso, Jorge Sanchez-Molina, Maria Rodriguez-Hidalgo, Araceli Lara-Lopez, Javier Ruiz-Ederra
Summary: Inherited retinal dystrophies (IRDs) affect a significant number of people worldwide, and gene therapy has shown potential in improving visual function for these patients. Subretinal injection is becoming popular among researchers and clinicians as a method to effectively deliver viral vectors for gene therapy. This review aims to provide insight into current subretinal injection techniques and understand the key factors for success in this procedure.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Maria Llorian-Salvador, Eimear M. Byrne, Manon Szczepan, Karis Little, Mei Chen, Heping Xu
Summary: The study found that complement activation plays a crucial role in the development of macular fibrosis, partially through the C5a-C5aR-mediated epithelial-to-mesenchymal transition (EMT). Therefore, targeting complement activation rather than C5a may be a novel approach for the management of macular fibrosis.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Cell Biology
Lei Wang, Jiani Cao, Yaojin Peng, Boqiang Fu, Zibing Jin, Yuntao Hu, Wei Wu, Andy Peng Xiang, Shijun Hu, Junying Yu, Yu Zhang, Jun Wei, Yong Zhang, Qiyuan Li, Jiaxi Zhou, Peijun Zhai, Huanxin Zhu, Lingmin Liang, Aijin Ma, Glyn Stacey, Tongbiao Zhao, Jie Hao
Summary: The guidelines for human retinal pigment epithelial cells in China were jointly drafted by experts from the Chinese Society for Stem Cell Research, aiming to improve quality control and accelerate international standardization.
CELL PROLIFERATION
(2022)
Article
Ophthalmology
M. Helena Cardoso, Michael J. Hall, Thomas Burgoyne, Pedro Fale, Tina Storm, Cristina Escrevente, Pedro Antas, Miguel C. Seabra, Clare E. Futter
Summary: This study aimed to model the effects of chloroquine on the retinal pigment epithelium in vivo and investigate the impact of mild chloroquine treatment on lysosome function and turnover. The research found that low-dose chloroquine treatment inhibits lysosome reformation, leading to a gradual depletion of lysosomes' ability to interact with cargo-carrying vacuoles and degrade their content. The accumulation of pro-cathepsin D and LAMP1 reflects the inhibition of normal turnover of lysosomal constituents and possibly lysosomes themselves. Understanding the mechanisms underlying lysosome reformation may contribute to the development of new treatments for chloroquine-induced retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Kang V. Li, Miguel Flores-Bellver, Silvia Aparicio-Domingo, Carson Petrash, Hannah Cobb, Conan Chen, M. Valeria Canto-Soler, Marc T. Mathias
Summary: Transplantation of stem cell-derived RPE cells is a promising therapy for retinal degenerative diseases. This study presents a set of clinically applicable devices for the subretinal implantation of RPE grafts. The devices demonstrated biocompatibility and high preservation of RPE transplants, and the surgical device allowed precise delivery of the transplants without causing damage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Ophthalmology
Han Joo Cho, Young Joon Jeon, Wontae Yoon, Jihyun Lee, Jaemin Kim, Chul Gu Kim, Jong Woo Kim
Summary: The study analyzed the clinical characteristics of drusenoid pigment epithelial detachment (PED) with subretinal fluid (SRF) and evaluated the impact of SRF on long-term visual and anatomical outcomes. The results showed that drusenoid PED with SRF had larger height, diameter, and volume compared to those without SRF, but there was no significant difference in best-corrected visual acuity at the final visit between the two groups. Additionally, the incidence of retinal pigment epithelial and outer retinal atrophy (cRORA) and macular neovascularization (MNV) did not differ significantly between the two groups.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2023)
Article
Cell Biology
Jinying Li, Chen Qiu, Yang Wei, Weixin Yuan, Jia Liu, Wenyu Cui, Jiayi Zhou, Cong Qiu, Lihe Guo, Liquan Huang, Zhen Ge, Luyang Yu
Summary: Human amniotic epithelial stem cells can differentiate into RPE like cells with the help of trichostatin A and nicotinamide, showing potential therapeutic effects for age-related macular degeneration. Subretinal transplantation of these cells in RCS rats resulted in rescued visual function and retinal structure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Eabha O'Driscoll, Emily Hughes, Mustapha Irnaten, Markus Kuehn, Deborah Wallace, Colm O'Brien
Summary: Optic nerve head cupping, a feature of glaucoma, is associated with extracellular matrix remodeling and lamina cribrosa fibrosis. Peripapillary atrophy is common in glaucoma and involves the loss of retinal pigment epithelium adjacent to the optic nerve head. Under pro-fibrotic conditions, epithelial cells can undergo epithelial-to-mesenchymal transition and contribute to extracellular matrix fibrosis. This study investigates the role of this process in glaucoma and peripapillary atrophy, demonstrating the presence of retinal pigment epithelium cells in glaucomatous optic nerve heads and the potential of these cells to differentiate into fibroblast-like cells.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Fatma Sogut, Mahmut Akilli, Ayse Humeyra Kaynar, Handan Tuncel, Deniz Kibar, Sakir Necat Yilmaz, Ulku Comelekoglu
Summary: In this study, the interaction between silica nanoparticles and ion channels in retinal pigment epithelial (RPE) cells was observed using the whole-cell patch clamp technique. The results showed that the windowed scalogram entropy method was sensitive to the effect of silica nanoparticles on RPE cell TRP currents. Additionally, the windowed scale index method could analyze the temporal fluctuations in the aperiodicity of cell membrane current signals. The findings suggest that entropy measurement methods may be useful for the function analysis of cell ion channels.
MEDICAL HYPOTHESES
(2023)
Article
Materials Science, Multidisciplinary
Biola F. Egbowon, Enzo Fornari, Joseph M. Pally, Alan J. Hargreaves, Bob Stevens, T. Martin Mcginnity, Barbara K. Pierscionek
Summary: This study demonstrates that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without or with fluocinolone acetonide (FA) treatment. The cells survived and maintained their morphology for up to 150 days with FA-treated ENS, showing an epithelial phenotype with biomarkers critical for retinal characteristics. This technique has potential for subretinal transplantation.
MATERIALS & DESIGN
(2023)
Article
Medicine, Research & Experimental
Jean-Baptiste Ducloyer, Virginie Pichard, Mathieu Mevel, Anne Galy, Gaelle M. Lefevre, Nicole Brument, Dimitri Alvarez-Dorta, David Deniaud, Alexandra Mendes-Madeira, Lyse Libeau, Caroline Le Guiner, Therese Cronin, Oumeya Adjali, Michel Weber, Guylene Le Meur
Summary: This study evaluated the distribution of enhanced green fluorescent protein (EGFP) after subretinal injection in non-human primates. The results showed that air tamponade resulted in a wider diffusion of EGFP, while without air tamponade, EGFP expression was limited to the injection site. This finding is important for improving the safety and efficacy of subretinal gene therapy.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Masahiro Miura, Shuichi Makita, Yoshiaki Yasuno, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi
Summary: The study quantitatively evaluated retinal pigment epithelium changes in patients with age-related macular degeneration using prototype multi-contrast optical coherence tomography. The results showed that multi-contrast OCT is useful for objective evaluation of changes in the retinal pigment epithelium.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Andrew W. Taylor, Samuel Hsu, Tat Fong Ng
Summary: The retinal pigment epithelial cells (RPE) in the ocular tissue microenvironment play a crucial role in regulating immune cells by expressing immunomodulating molecules to suppress inflammation and promote immune tolerance. Understanding how RPE mediate this regulation and the changes seen under pathological conditions is important in the study of ocular immune privilege.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Ophthalmology
Frederick A. Jakobiec, Paula Cortes Barrantes, Yoshihiro Yonekawa, Eleonora M. Lad, Alan D. Proia
Summary: The study evaluated mononuclear cells in the subretinal exudate of patients with Coats' disease, finding that most of these cells were CD163(+) and a minority were RPE65(+). The expression of CD163 and RPE65 in the retinal pigment epithelium supports differentiation toward histiocytes, with potential implications for macular degeneration.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Cell Biology
Ying Su, Ziyan Tang, Feng Wang
Summary: The study investigated the regulation of LINC01592 on human RPE cells undergoing epithelial-mesenchymal transition induced by TGF-beta 1, finding that LINC01592 overexpression enhanced the process while its inhibition reduced it. This provides a novel treatment approach for proliferative vitreoretinopathy.
Review
Chemistry, Multidisciplinary
Kai Wang, Zongchao Han
JOURNAL OF CONTROLLED RELEASE
(2017)
Article
Engineering, Biomedical
Rajendra Narayan Mitra, Min Zheng, Ellen R. Weiss, Zongchao Han
Article
Nanoscience & Nanotechnology
Ryan A. Kelley, Shannon M. Conley, Rasha Makkia, Jamie N. Watson, Zongchao Han, Mark J. Cooper, Muna I. Naash
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2018)
Article
Engineering, Biomedical
Kai Wang, Rajendra Narayan Mitra, Min Zheng, Zongchao Han
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2018)
Article
Chemistry, Multidisciplinary
Ruijuan Gao, Rajendra Narayan Mitra, Min Zheng, Kai Wang, Jesse Christine Dahringer, Zongchao Han
ADVANCED FUNCTIONAL MATERIALS
(2018)
Article
Multidisciplinary Sciences
Kai Wang, Min Zheng, Kaitlyn Lee Lester, Zongchao Han
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Multidisciplinary
Fan Yu, Min Zheng, Alice Yang Zhang, Zongchao Han
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Biotechnology & Applied Microbiology
Min Zheng, Rajendra N. Mitra, Ellen R. Weiss, Zongchao Han
Article
Multidisciplinary Sciences
Biraj Mahato, Koray Dogan Kaya, Yan Fan, Nathalie Sumien, Ritu A. Shetty, Wei Zhang, Delaney Davis, Thomas Mock, Subrata Batabyal, Aiguo Ni, Samarendra Mohanty, Zongchao Han, Rafal Farjo, Michael J. Forster, Anand Swaroop, Sai H. Chavala
Article
Chemistry, Multidisciplinary
Yong-Su Kwon, Min Zheng, Alice Yang Zhang, Zongchao Han
Summary: Melanin is a natural pigment with antioxidant and photoprotective properties. However, its protective effects decline with age, contributing to the development of age-related macular degeneration (AMD). To enhance the effects of melanin, researchers developed synthetic melanin-like nanoparticles (MNPs) that can replace natural melanin in the retinal pigment epithelium (RPE). These MNPs have demonstrated biocompatibility, powerful antioxidant properties, and the ability to selectively target reactive oxygen species (ROS) in cellular and mouse models of AMD. The findings suggest the potential efficacy of MNPs as a natural radical scavenger in ROS-related diseases like AMD.
Article
Biochemistry & Molecular Biology
Simna Sp, Rajendra N. Mitra, Min Zheng, Jared D. Chrispell, Kai Wang, Yong-Su Kwon, Ellen R. Weiss, Zongchao Han
Summary: Delivery of a nanoparticle-mediated full-length mouse or human genomic rhodopsin locus can effectively rescue rod photoreceptor degeneration in a mouse model of autosomal dominant retinitis pigmentosa. The use of an intact genomic locus as a transgene is more successful compared to cDNA treatment, highlighting the significance of gene augmentation using regulatory elements.
Article
Pharmacology & Pharmacy
Kai Wang, Min Zheng, Charles Askew, Xintao Zhang, Chengwen Li, Zongchao Han
Summary: In this study, thermoresponsive polymer elastin-like polypeptides (ELPs) were used to enhance the gene therapy effect of Adeno-associated virus (AAV), resulting in significant improvement in expression levels of AAV at high neutralizing antibody titers. The ELP-AAV nanoparticles were stable and had a strong potential to improve gene delivery without the need for redesigning the AAV virion.
ADVANCED THERAPEUTICS
(2022)
Article
Oncology
Kai Wang, Yuhui Chen, Sarah Ahn, Min Zheng, Elisa Landoni, Gianpietro Dotti, Barbara Savoldo, Zongchao Han
Article
Ophthalmology
Priyadarsini Asokan, Rajendra N. Mitra, Ramesh Periasamy, Zongchao Han, Teresa Borras
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Article
Chemistry, Multidisciplinary
Rajendra N. Mitra, Ruijuan Gao, Min Zheng, Ming-Jing Wu, Maxim A. Voinov, Alex I. Smirnov, Tatyana I. Smirnova, Kai Wang, Sai Chavala, Zongchao Han