期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 216, 期 -, 页码 105-112出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.03.094
关键词
Electrical impedance spectroscopy; Microfabrication; Alumina nanopore membrane; DNA sensor
资金
- Swiss National Foundation project [FNS 200021-140493]
In this work we demonstrate microfabricated thin alumina nanopore membranes as a platform for impedance sensing of DNA immobilization and hybridization. We develop a wafer-scale fabrication of free-standing alumina nanopore membranes with well controlled thickness, pore diameter and overall pore density. One 1 cm x 1 cm single chip contains an array of 69 membranes. Each membrane is 100 mu m x 100 mu m large and 2 mu m thick, with pore diameter of 120 nm. With low pore density of similar to 6 pores/mu m(2), nanopore resistance and membrane capacitance can be recognized clearly in the electrochemical impedance spectrum from 100 to 1 MHz. The total surface area can be further increased by the coating of silica nanoparticles with similar to 20 nm in diameter. During the immobilization of probe ssDNA to (3-glycidoxypropyl) trimethoxysilane functionalized surface, the nanopore resistance drops significantly by 80%, whereas the membrane capacitance increases less than 2%. After hybridization with complementary DNA, the nanopore resistance increases up to 10%. Non-complementary ssDNA has no obvious effect. The detection limit is 12.5 nM in phosphate-buffered saline (PBS) solution. (C) 2015 Elsevier B.V. All rights reserved.
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