Article
Nanoscience & Nanotechnology
Tomoki Hayashida, Makusu Tsutsui, Sanae Murayama, Tomoko Nakada, Masateru Taniguchi
Summary: This study systematically characterized the effect of dielectric coatings on the capture-to-translocation dynamics of single particles in solid-state pores. By covering SiNx membranes with different coatings, the researchers were able to change the zeta-potential at the pore wall, leading to easier particle capture and slower translocation motion in the channel. These findings serve as a guide for engineering pore wall surfaces to optimize translocation dynamics for efficient sensing of particles and molecules.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Materials Science, Multidisciplinary
Anupama Rao Gulur Srinivas, Rafiq Hilali, Mona Damavandi, Jenny Malmstrom, David Barker, Eva Weatherall, Geoff Willmott, Jadranka Travas-Sejdic
Summary: This study successfully achieved the functionalization of TPU tunable nanopores using a one-pot strategy, with grafting of negatively charged PAA brushes through the Graftfast method and demonstration of grafting of neutral brushes. The functionalization was characterized using various analytical methods, confirming the increase in negative surface charge on the pore walls.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Dario Dematties, Chenyu Wen, Mauricio David Perez, Dian Zhou, Shi-Li Zhang
Summary: The study utilizes deep learning for feature extraction using a bipath network (B-Net), which acquires prototypical pulses and the ability to recognize and extract features without pre-assigned parameters. Evaluation on simulated and experimental data shows small relative errors and stable trends in the B-Net results. The B-Net is capable of processing data with a signal-to-noise ratio equal to 1, something not possible for threshold-based algorithms.
Review
Biochemistry & Molecular Biology
Laxmicharan Samineni, Bibek Acharya, Harekrushna Behera, Hyeonji Oh, Manish Kumar, Ratul Chowdhury
Summary: Proteins are crucial for cellular functions and their structures can be engineered for industrial applications. This article reviews successful cases of protein engineering in pore-forming proteins and discusses their applications in nucleotide/peptide sequencing, single-molecule sensing, and molecular separations.
Article
Chemistry, Analytical
Essraa A. Hussein, Ryan J. White
Summary: Resistive pulse sensing using ion channel proteins has evolved as a single-molecule approach with high molecular selectivity, but requires a stable lipid membrane. The newly developed gold electrode-supported bilayer technology offers high spatial resolution but suffers from channel current decay. Introducing a silver nanoneedle to support lipid bilayer formation and ion channel measurements can mitigate current decay and produce stable DC channel currents.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hye-Jin Hwang, Jin-Sik Kim, Jeonghyun Lee, Jun Sik Min, Ki-Baek Jeong, Eunha Kim, Mi-Kyung Lee, Seung-Wook Chi
Summary: Nanopore sensors are a promising platform for single-molecule sensing, such as sequencing, disease diagnostics, and drug screening. In this study, the interaction between Bcl-xL and Bak-BH3 was characterized using chemically modified OmpG nanopores, and specific inhibition of Bcl-xL was achieved using a small-molecule antagonist. The results demonstrate that chemically modified OmpG nanopores can serve as a valuable sensor platform for ultrasensitive, rapid, and single-molecule-based drug screening against protein-protein interactions.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Kaan Kececi, Dila Kaya, Charles R. Martin
Summary: This study presents a simple method to detect 100-bp DNA using resistive-pulse sensing and investigates the effects of analyte concentration, potential, tip size, and electrolyte concentration. The findings show that current-pulse events can be observed at low potentials and exhibit linear concentration dependence. Additionally, the study explores the impact of tip size on DNA translocation frequency.
Article
Multidisciplinary Sciences
Iat Wai Leong, Shohei Kishimoto, Makusu Tsutsui, Masateru Taniguchi
Summary: This study reports on the interference of ion diffusion kinetics at liquid-electrode interfaces in nanopore sensing. The growing impedance at the liquid-metal interfaces was found to significantly affect the measurements of ionic current, leading to decreased stability and temporal resolution.
Article
Chemistry, Multidisciplinary
Sumanth K. Maheshwaram, Divya Shet, Serene R. David, Mahesh B. Lakshminarayana, Gautam V. Soni
Summary: In this study, a nanopore platform was used in combination with a voltage-dependent force rupture assay to detect distinct structures on nucleosomal arrays and induce breakdown of individual nucleosome complexes. The results showed that nucleosomes exist as a combination of complete and partial structures, and the partial nucleosomes result from voltage-dependent structural disintegration. The study also quantified the heterogeneity and nucleosomal substructure sizes on the arrays.
Article
Chemistry, Analytical
Xiaoqiong Chen, Qiang Zeng, Rui Zhang, Xuye Liu, Min Wang, Lishi Wang
Summary: This paper investigates the translocation events of single ellipsoid human serum albumin (HSA) through a solid glass nanopore using resistive pulses. The events can be classified into three groups based on the difference in current pulse amplitudes. By adjusting the electric field of the nanopore under different conditions, such as voltage, pH, and pore size, it is found that the observation is primarily associated with dynamic rotation rather than increased size exclusion of HSA. The rotation angles of translocated HSA are determined through experimental and theoretical studies.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Chien Hsu, Chih-Yuan Lin, Amer Alizadeh, Hirofumi Daiguji, Wei-Lun Hsu
Summary: This study theoretically analyzes the electrophoretic behavior of spherical particles near the entrance of a charged nanopore and summarizes the dominant mechanisms governing particle behavior for different conditions.
Article
Nanoscience & Nanotechnology
Ziyu Han, Jiantao Liu, Zhanning Liu, Wenwei Pan, Yang Yang, Xuejiao Chen, Yunhua Gao, Xuexin Duan
Summary: This study introduces an IC-compatible method for fabricating a PDMS-based resistive pulse sensing device with embedded nanochannels, allowing for label-free analysis of biomolecules and bionanoparticles. The device was successfully tested with polystyrene micro- and nanoparticles, as well as for plasmid DNA detection and exosome analysis, demonstrating its potential for quantification of nanoscale biomolecules. The proposed nanochannel-RPS device and sensing strategy offer a cost-effective solution for large-scale manufacturing and accurate analysis of nanoscale particles and biomolecules.
Article
Mechanics
Ali Shafiei Souderjani, Mostafa Bakouei, Mohammad Hassan Saidi, Mojtaba Taghipoor
Summary: The electrophoretic motion of hydrophobic particles in solid-state nanopores is numerically investigated. The Poisson, Stokes, and Nernst-Planck equations are simultaneously solved using the Newton-Raphson algorithm. The hydrophobic surface characteristics are described using the Navier-slip boundary condition with a wide range of slip lengths. The study examines the effects of electric field intensity, electrolyte concentration, and particle size on electrophoretic velocity, and manipulates the nanopore's size and surface charge density to separate hydrophobic and hydrophilic particles based on their slip lengths. The resistive pulse properties of particles with different slip lengths are also studied, showing potential application for resistive pulse sensing to determine particle slip length.
Article
Multidisciplinary Sciences
Lauren S. Lastra, Y. M. Nuwan D. Y. Bandara, Michelle Nguyen, Nasim Farajpour, Kevin J. Freedman
Summary: This article investigates the translocation mechanism, pulse direction, and shape in nanopore sensing, and proposes the role of electroosmosis in driving DNA events and conductive events.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Inorganic & Nuclear
Naini Garg, Akash Deep, Amit L. Sharma
Summary: Metal-organic Frameworks (MOFs) are porous crystalline structural materials made up of metal ions and organic linkers, offering endless combinations for various applications such as sensing, gas storage, catalysis, optoelectronics, and drug delivery. MOF nanostructures are being developed to incorporate beneficial attributes and overcome the weaknesses of individual components, leading to high-performance chemo-resistive gas sensors and other advanced applications.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Analytical
Kaan Kececi, Nevim San, Dila Kaya
Article
Electrochemistry
Fatih Buyukserin, Charles R. Martin
ELECTROCHIMICA ACTA
(2016)
Article
Electrochemistry
Dila Kaya, Ali Dinler, Nevim San, Kaan Kececi
ELECTROCHIMICA ACTA
(2016)
Article
Materials Science, Biomaterials
Sevde Altuntas, Fatih Buyukserin, Ali Haider, Buket Altinok, Necmi Biyikli, Belma Aslim
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2016)
Article
Chemistry, Analytical
Merve Celik, Sevde Altuntas, Fatih Buyukserin
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Nanoscience & Nanotechnology
Gozde Ozaydin Ince, Efe Armagan, Hakan Erdogan, Fatih Buyukserin, Lokman Uzun, Gokhan Demirel
ACS APPLIED MATERIALS & INTERFACES
(2013)
Article
Chemistry, Physical
Sevde Altuntas, Fatih Buyukserin
APPLIED SURFACE SCIENCE
(2014)
Article
Biochemical Research Methods
Sercan Keskin, Esen Efeoglu, Kaan Kececi, Mustafa Culha
JOURNAL OF BIOMEDICAL OPTICS
(2013)
Article
Chemistry, Multidisciplinary
Bihter Daglar, Gokcen Birlik Demirel, Tural Khudiyev, Tamer Dogan, Osama Tobail, Sevde Altuntas, Fatih Buyukserin, Mehmet Bayindir
Article
Chemistry, Multidisciplinary
Yixian Wang, Kaan Kececi, Jeyavel Velmurugan, Michael V. Mirkin
Article
Chemistry, Multidisciplinary
Yixian Wang, Kaan Kececi, Michael V. Mirkin, Vigneshwaran Mani, Naimish Sardesai, James F. Rusling
Article
Chemistry, Multidisciplinary
F. Buyukserin, S. Altuntas, B. Aslim
Article
Materials Science, Multidisciplinary
Bihter Daglar, Tural Khudiyev, Gokcen Birlik Demirel, Fatih Buyukserin, Mehmet Bayindir
JOURNAL OF MATERIALS CHEMISTRY C
(2013)
Article
Chemistry, Multidisciplinary
Dila Kaya, Kaan Kececi, Durdane Yilmaz
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
D. Kaya, K. Kececi
BULGARIAN CHEMICAL COMMUNICATIONS
(2017)