Article
Biochemistry & Molecular Biology
Florian Labourel, Etienne Rajon
Summary: Enzyme efficiency is influenced by competition for resources and undergoes directional selection up to a certain point before evolving near-neutrality. Evolutionary outcomes for enzymes vary due to differences in the evolutionary context encountered by each enzyme, with drift and effective population size playing important roles. Joint evolution of enzyme concentration and efficiency does not lead to extensive variance in outcomes, suggesting that other factors such as the kinetics of nutrient transporters, tolerance to high concentrations of metabolites, and reversibility of reactions are also significant.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biology
Lambertus A. A. Peletier, Johan Gabrielsson
Summary: This paper explores the impact of enzyme turnover on the classical Michaelis-Menten model. The results show that enzyme turnover significantly alters the dynamics of substrate, free- and bound enzyme, and impacts the rate of substrate conversion to metabolite.
MATHEMATICAL BIOSCIENCES
(2022)
Article
Chemistry, Physical
Rumasha N. T. Kankanamage, Ben K. Ahiadu, Jie He, James F. Rusling
Summary: Enzymes serve as effective catalysts in organic syntheses, offering high selectivity that is often not achievable with chemical catalysts. Biocatalysis with iron heme enzymes in thin films has demonstrated improved stability and efficiency. Utilizing polylysine-crosslinked horseradish peroxidase (HRP) coatings on magnetic beads, selective synthesis of single nitro-phenol products was achieved for a series of para-substituted phenols. This approach shows promise as a valuable tool for biocatalytic chemical synthesis, with significantly improved turnover numbers at elevated temperatures.
Article
Food Science & Technology
Juan Blanco, Helena Martin, Carmen Marino, Araceli E. Rossignoli
Summary: The study investigated the depuration kinetics of okadaic acid in cockles and found that cockles depurated the toxin faster than most other bivalve species. Two-compartment models were needed to describe the depuration process effectively, and esters were depurated at a faster rate than the free form of the toxin.
Article
Agronomy
Carmen Yam-Chimal, Luis Ramirez-Y-Aviles, Jorge Navarro-Alberto, Francisco Solorio-Sanchez, Eduardo Villanueva-Couoh, Jose Noh-Medina
Summary: The application of enzymatic kinetics theory on the nutrition of horticultural species is limited. I-max and K-m were determined for habanero pepper and their values provided information about the efficient use of phosphorus in plants. The roots of habanero pepper can uptake phosphorus efficiently from the soil.
Article
Chemistry, Analytical
Shengmei Tai, Zhijuan Qian, Hongxin Ren, Alberta Osei Barimah, Chifang Peng, Xinlin Wei
Summary: A colorimetric detection method for Glyp was developed using beta-CD@DNA-CuNCs enzyme mimics, showing high sensitivity and selectivity. The method established by detecting the changes of absorption signal at 652 nm provides a potential approach for monitoring Glyp residue in food.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Yu Zhang, Ying Zhang, Miaojia Song, Evangelos Topakas, Qiang Yu, Zhenhong Yuan, Zhongming Wang, Ying Guo
Summary: Conversion of lignocellulose to sugar relies on costly cellulase and xylanase, therefore, improving enzyme utilization is crucial. Enzymatic kinetics can guide researchers to efficient enzyme utilization, with models based on second-order reactions being superior to first-order ones. Enzyme activity and sugar yield have a linear relationship, while higher enzyme loading leads to reduced activity retention.
PROCESS BIOCHEMISTRY
(2021)
Article
Microbiology
Karim Fahmy
Summary: Quantitative analysis of cell replication explores the relationship between metabolism and growth by proposing a growth model with enthalpy as a variable. Isothermal microcalorimetry traces show consistent patterns in the enthalpy domain, which align with a Langmuir adsorption reaction model. The proposed formalism accurately captures the lifespan of cultured microorganisms from exponential growth to metabolic decline.
Article
Chemistry, Multidisciplinary
Chathuri J. Kombala, Sanjaya D. Lokugama, Aikaterini Kotrotsou, Tianzhe Li, Alyssa C. Pollard, Mark D. Pagel
Summary: A newly developed CEST agent can simultaneously measure pH and evaluate protease activity, with the ability to determine pH values through selective saturation signals and ratio analysis.
Article
Multidisciplinary Sciences
Maryam Khan, Zubair Ahmad, Farhad Ali, Naveed Khan, Ilyas Khan, Kottakkaran Sooppy Nisar
Summary: This research focuses on the modelling and dynamics of enzyme processes based on chemical kinetics. A two-step reversible reaction of substrate-enzyme is investigated and transformed into a fractional-order model using the Atangana-Baleanu operator and Mittag-Leffler kernel. The numerical results obtained through the Atangana-Toufik method show the dynamics of the reaction against different reaction rates.
Article
Nanoscience & Nanotechnology
Rong Liu, Dajiao Cheng, Quan Zhou, Fushuang Niu, Ke Hu
Summary: Synthetic nanomaterials known as nanozymes can catalyze biological transformations, and photonanozymes utilize light for spatial and temporal control. A gold nanocluster modified with glutathione serves as a photonanozyme, catalyzing the reduction of nitrobenzene under light and showing potential as a biocompatible catalyst in mammalian cells. This study reinvents gold nanoclusters as photonanozymes, mimicking natural nitroreductases for potential prodrug activation.
ACS APPLIED NANO MATERIALS
(2021)
Article
Environmental Sciences
Elena Gogina, Igor Gulshin
Summary: This study investigated the operational stability of laboratory oxidation ditches for synthetic and real wastewater under low dissolved oxygen concentrations. The research showed that the efficiency of nitrification and denitrification can be improved by the generation of large floccules of activated sludge in the oxidation ditches. By determining the size of sludge floccules and the main functional microbial groups, the obtained reaction rates can be utilized for calculations and simulations of oxidation ditches operating at low dissolved oxygen concentrations.
Article
Soil Science
Petr Capek, Christopher P. Kasanke, Robert Starke, Qian Zhao, Karolina Tahovska
Summary: The competitive inhibition of MUB-P decay by P-PO43- and dissolved organic P was found, leading to an underestimation of potential acid phosphatase activity by up to 20%. To avoid bias, it is recommended to analyze progress curves at a wide range of initial MUB-P concentrations using non-linear methods when optimizing the enzyme assay for a given soil.
BIOLOGY AND FERTILITY OF SOILS
(2021)
Article
Biochemistry & Molecular Biology
Bjarte Aarmo Lund, Bjorn Olav Brandsdal
Summary: A new method was developed to efficiently determine the temperature dependence of enzyme catalysis, requiring less sample, labor, and time. The method was validated with Bacillus pumilus LipA enzyme and three different enzymes, effectively differentiating between enzyme types at various temperatures.
Review
Biochemistry & Molecular Biology
Todd P. Silverstein
Summary: Two influential enzymological theories were proposed in the late 1970s - that catalytic power can stem from ground state destabilization and enzyme-substrate binding thermodynamics are independent of initial substrate concentration. Nonspontaneous binding can only occur under standard state conditions when c(0) is defined to be less than Km.
BIOPHYSICAL CHEMISTRY
(2021)
Review
Polymer Science
Brian J. Melde, Anthony P. Malanoski, Martin H. Moore, Brandy J. Johnson
Summary: Covalently attached liquids provide a liquid-liquid surface interaction for contacting contaminants, with liquid-like mobility and repellency properties. These coatings have the potential to enhance chemical resistance and prevent chemical contamination, offering unique advantages in comparison to other types of polymer chains.
POLYMER INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Jeffrey S. Erickson, Brandy J. Johnson, Anthony P. Malanoski
Review
Chemistry, Analytical
Brandy J. Johnson, Anthony P. Malanoski, Jeffrey S. Erickson
Article
Chemistry, Multidisciplinary
Zofia E. Siwicka, Florencia A. Son, Claudia Battistella, Martin H. Moore, Joanna Korpanty, Naneki C. McCallum, Zheng Wang, Brandy J. Johnson, Omar K. Farha, Nathan C. Gianneschi
Summary: Melanin, commonly known for its role in skin pigment and UV radiation protection, has been found to have the ability to adsorb toxins in some melanized organisms. A synthetic porous melanin (SPM) was successfully developed with the highest measured surface area among polydopamine-based materials, showing effectiveness in absorbing various gases and organophosphate toxins with high selectivity and potential for ammonia capture. The discovery of porous analogues in biological systems is anticipated due to the demonstrated advantages of synthetic porous melanin and melanin's natural adsorbent role.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biotechnology & Applied Microbiology
Rebecca L. Mickol, Brian J. Eddie, Anthony P. Malanoski, Matthew D. Yates, Leonard M. Tender, Sarah M. Glaven
Summary: The study characterized the CANode biofilm using metagenomics and metatranscriptomics and found that Desulfobulbaceae-2 may be a key player in both energy-generating and energy storage reactions. This suggests that the CANode system can be viewed as a living rechargeable battery.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biology
Daniel A. Phillips, Lori A. Zacharoff, Cheri M. Hampton, Grace W. Chong, Anthony P. Malanoski, Lauren Ann Metskas, Shuai Xu, Lina J. Bird, Brian J. Eddie, Aleksandr E. Miklos, Grant J. Jensen, Lawrence F. Drummy, Mohamed Y. El-Naggar, Sarah M. Glaven
Summary: BAR domain proteins are a superfamily of coiled-coil proteins that influence membrane curvature in eukaryotes, and a bacterial protein BdpA with BAR domain-like activity has been discovered to impact the formation of membrane vesicles and outer membrane extensions. BdpA is essential for the uniform size distribution of membrane vesicles and the scaffolding of consistent diameter and curvature in OMEs, influencing the morphology of these structures. Overexpression of BdpA can promote the formation of OMEs in bacterial species where it is not typically observed, suggesting a new class of bacterial BAR domain-like proteins.
Article
Microbiology
Scott N. Dean, Jerome Anthony E. Alvarez, Dan Zabetakis, Scott A. Walper, Anthony P. Malanoski
Summary: The study introduces the PepVAE framework for designing novel AMPs using VAE and antimicrobial activity prediction models. By sampling from different regions of the learned latent space, new AMP sequences can be generated with minimal input parameters for controlled production of AMPs with predicted antimicrobial activity. This modular design framework shows promise for development of novel AMPs with experimental validation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Materials Science, Biomaterials
Joseph R. Spangler, Denver N. Cooper, Anthony P. Malanoski, Scott A. Walper
Summary: Quorum sensing (QS) systems in bacteria have been repurposed for biosensors and inducible protein expression. By altering promoter activity, an engineered QS sentinel organism capable of detecting specific pathogen-associated signaling molecules has been developed.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Microbiology
Brian J. Eddie, Lina J. Bird, Claus Pelikan, Marc Mussmann, Clara Martinez-Perez, Princess Pinamang, Anthony P. Malanoski, Sarah M. Glaven
Summary: A well-conserved pathway for extracellular electron uptake is identified in the order Tenderiales, suggesting that electroautotrophy may be pervasive in this group of bacteria. The geographic locations from which metagenome-assembled genomes were recovered offer insights into the natural ecological niche of the Tenderiales.
Article
Microbiology
Brian J. Eddie, Anthony P. Malanoski, Elizabeth L. Onderko, Daniel A. Phillips, Sarah M. Glaven
Summary: Marinobacter atlanticus produces small amounts of electrical current on electrodes, which is enhanced by the addition of redox mediators; RNA-sequencing revealed increased expression of genes for molybdate transport and type VI secretion system in cells grown at current-producing potentials.
Proceedings Paper
Engineering, Electrical & Electronic
Anthony P. Malanoski, Jeffrey S. Erickson, Brandy J. Johnson, Earl Tankard
Article
Microbiology
Lina Bird, Brian J. Eddie, Anthony P. Malanoski, Princess Pinamang, Sarah M. Glaven
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Chemistry, Multidisciplinary
Xintong Zhang, Yanhong Liu, Wei Liu, Liqing Chen, Mingji Jin, Zhonggao Gao, Wei Huang
Summary: This study developed a macrophage-hitchhiking gene delivery system for the treatment of rheumatoid arthritis. The system demonstrated excellent targeting ability and nuclear entry ability, leading to efficient transfection of interleukin-10 in macrophages and alleviation of inflammation symptoms. This research provides a novel strategy for gene therapy and gene delivery system design for rheumatoid arthritis and other similar inflammatory diseases.
Article
Chemistry, Multidisciplinary
Richard K. Cross, Dave Spurgeon, Claus Svendsen, Elma Lahive, Simon Little, Frank von der Kammer, Frederic Loosli, Marianne Matzke, Teresa F. Fernandes, Vicki Stone, Willie J. G. M. Peijnenburg, Eric A. J. Bleeker
Summary: Even small changes in physicochemical properties of nanoforms (NFs) can influence their environmental fate and hazard. Testing and characterizing each individual NF will not be feasible due to the large number of new materials being developed. Targeting the most relevant form of the NF for a given exposure is important for efficient risk assessment. In aquatic systems, functional fate processes play a key role in determining the exposure relevant form of NFs. Grouping of NFs and read-across based on functional fate pathways can be justified by considering the shared fate and hazard profile. A new Integrated Approaches to Testing and Assessment (IATA) is presented, focusing on dissolution, dispersion stability, chemical transformations, and the contribution to toxicity from particles and dissolved components. This IATA can be used as a template for future in vivo kinetic assessments.
Article
Chemistry, Multidisciplinary
Minhui Lu, Xiaoxuan Zhang, Lijun Cai, Jingjing Gan, Jinglin Wang, Yu Wang, Yuanjin Zhao
Summary: Researchers have proposed a novel black phosphorus-loaded hydrogel inverse opal microneedle patch that exhibits photothermal responsive capacity and vivid structural color screening for psoriasis treatment. With improved materials, structures, and functions, the microneedle patch enables intelligent drug delivery and enhances drug loading and controllable release.
Article
Chemistry, Multidisciplinary
Qianyun Tang, Dandan Wang, Jinhui Cui, Yiheng Zhang, Junyang Mei, Jing Du, Anyue Xia, Qian Sun, Dan Luo, Baosan Han, Mingzhe Gan, Peifeng Liu
Summary: This study presents a novel microfluidic platform for precise and flexible control of oxygen concentrations in microbial suspension culture. The platform demonstrates unique capabilities for spatiotemporal gas control and detection, allowing for applications in screening, studying, and culturing industrial or niche-specific environmental microbiomes.
Review
Chemistry, Multidisciplinary
Jiaen Wu, Hao Chen, Jiawei Xu, Muhammad Saif Ur Rahman, Shengmei Li, Jie Wang, Shifen Huang, Charles C. Han, Shanshan Xu, Ying Liu
Summary: This review categorizes the potential health risks of microplastic pollution by focusing on the three primary pollution sources. It provides an in-depth analysis of the pharmacokinetics, toxicity potential, and biological mechanism of microplastics in the human body. The review aims to fill knowledge gaps about the toxicity of microplastics on human health and provide ideas for repairing the damage caused by microplastics.
Article
Chemistry, Multidisciplinary
Fanshu Ma, Yi Cao, Jincong Yan, Zhongzhong Lu, Lina Sun, Zahid Hussain, Zheng Wang, Li Wang, Renjun Pei
Summary: This study proposes a simple yet powerful method to create multifunctional hybrid nanovesicles that combine the characteristics of oncolytic viruses and pyroptosis, leading to enhanced tumor targeting and improved immune response. The results demonstrate excellent tumor inhibition efficacy against melanoma and pulmonary metastasis.
Article
Chemistry, Multidisciplinary
Fangmian Wei, Johannes Karges, Siyuan Gao, Lili Wang, Xiting Zhang, Xing-Can Shen, Liangnian Ji, Hui Chao
Summary: This study presents the coordination of Ru(II) polypyridine complexes to graphitic carbon nitride nanosheets for oxygen-self-sufficient two-photon photodynamic immunotherapy. The conjugates were found with strong two-photon absorption and could generate reactive oxygen species (ROS) to induce cell death and inhibit tumor growth through immune system activation.
Article
Chemistry, Multidisciplinary
Weiwei Zheng, Shun-Yu Yao, Haijun Hu, Xiping Chen, Zhefeng Qian, Wenxing Liu, Yang Zhu, Zhengwei Mao, Dong-Sheng Guo, Changyou Gao
Summary: In this study, a hypoxia-responsive self-assembled peptide hydrogel was prepared for ischemic stroke treatment. The hydrogel showed the ability to release drugs and effectively improve motor function, reduce infarct volume, and alleviate inflammation.
Article
Chemistry, Multidisciplinary
Qianqian Qiao, Jinyu Wang, Kai Long, Linwei Li, Jiahao Chen, Yuhao Guo, Ziqiang Xu, Ying Kuang, Tianjiao Ji, Cao Li
Summary: This study developed a catalytic system using titanium-based MXene nanosheets to load enzymes and anticancer drugs. The nanosheets demonstrated catalase-like activity and photothermal capability, enabling enhanced cancer treatment through starvation therapy and alleviation of hypoxia. In vitro and in vivo studies confirmed the effective anticancer capability of this enzyme cascade system.
Article
Chemistry, Multidisciplinary
Shiyang Wu, Yan He, Ruiqi Zhou, Chunlin Chen, Dawei Chen, Haiyang Hu
Summary: In this research, LDHA@MIP-DSD nanoparticles were designed to enhance the effectiveness of immunogenic cell death (ICD) in cancer immunotherapy. LDHA@MIP-DSD improved the accessibility of nanodrugs to cancer cells by surface imprinting LDHA and induced autophagy with SLN. The combination of these two effects resulted in optimal immune stimulation and antitumor efficiency.
Article
Chemistry, Multidisciplinary
Xu Zhang, Kejian Shi, Jiahui Mao, Kerou Mao, Yangrui Jia, Jiakun Zhang, Qingzhen Wang, Ru Bai, Fene Gao, Shihui Liu, Mengyu Guo, Fenglan Qin, Shengmin Li, Chunying Chen, Huige Zhou, Jing Liu, Fulin Chen
Summary: Compared with vein injection, oral administration is a preferred non-invasive and self-help treatment option for cancer therapy. However, the harsh gastrointestinal tract and biological barriers limit the stability and efficiency of oral drug delivery systems. To overcome these challenges, researchers have developed Cyssome, a drug delivery platform that can maintain stability in harsh environments, penetrate biological barriers, and improve drug release and bioavailability.