期刊
NANO TODAY
卷 4, 期 3, 页码 235-243出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.nantod.2009.04.005
关键词
Nanowire; Field-effect transistor; Reversible surface functionalization; Protein-protein interaction
资金
- National Science Council of Taiwan [NSC 96-2627-M-002-003, 97-2627-M-002-019, 97-2627-M-001-002, 97-2627-M-002-020]
- NanoCore
- Core Facilities for Nanoscience and Nanotechnotogy at Academia Sinica
Taking advantage of the reversible association-dissociation between glutathione (GSH) and glutathione S-transferase (GST), we have developed a novel method by surface-functionalizing GSH on silicon nanowire field-effect transistors (SiNW-FET) and then anchoring a particular GST-fused protein for the studies of protein-protein interactions. The reversible surface functionalization method has been proven by analytical techniques, fluorescence imaging, atomic force microscopic imaging, and electrical measurements. The reversible GSH-GST functionality on the SiNW-FET has made this sensorial device consecutively reusable and calibratable, allowing for quantitative analysis. In this work, we have demonstrated that the biologically modified SiNW-FET can be used as a screening sensor for the future studies of biomolecular associations, such as protein-protein interactions, protein-DNA interactions, protein-carbohydrate interactions, etc. (C) 2009 Elsevier Ltd. All rights reserved.
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