Review
Biochemistry & Molecular Biology
Charles Bond, Adriana N. Santiago-Ruiz, Qing Tang, Melike Lakadamyali
Summary: Since its initial demonstration in 2000, there have been significant technological advancements in far-field super-resolution light microscopy. These developments have allowed for unprecedented visualization of the inner workings of cells. This review discusses the technical details of common implementations of super-resolution microscopy and highlights recent promising advances in the field.
Article
Multidisciplinary Sciences
Caio Vaz Rimoli, Cesar Augusto Valades-Cruz, Valentina Curcio, Manos Mavrakis, Sophie Brasselet
Summary: Single-molecule localization microscopy does not provide orientation information of proteins in cells. The authors propose a method called 4polar-STORM combining STORM with four-polarisation image splitting to measure single molecule orientation and wobbling in cells.
NATURE COMMUNICATIONS
(2022)
Article
Materials Science, Multidisciplinary
Zachary Hallenbeck, Esther A. Wertz
Summary: This work introduces a new technique that combines super-resolution microscopy with fluorescence lifetime imaging microscopy to study the enhancement of single-molecule decay rate by plasmonic nanoparticles. The technique provides high spatial resolution and temporal resolution, allowing for a better understanding of the mechanisms of plasmon-enhanced emission.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Biochemical Research Methods
David J. Schodt, Farzin Farzam, Sheng Liu, Keith A. Lidke
Summary: We introduce the Sequential Super-resolution Microscope (SeqSRM), a dedicated microscope for automated sequential localization microscopy. SeqSRM automates precise stage stabilization and data acquisition of user-selected cells, minimizing user interaction. We demonstrate that nanometer-scale changes in cell morphology can impact the fidelity of multi-target super-resolution reconstructions, but these shifts can be reliably detected and masked out using brightfield images. SeqSRM enables automated multi-target imaging without the need for multiple fluorophores, while accurately reflecting the relative organization of the targets.
BIOMEDICAL OPTICS EXPRESS
(2023)
Review
Food Science & Technology
Susana Dianey Gallegos-Cerda, Josue David Hernandez-Varela, Jose Jorge Chanona-Perez, Benjamin Arredondo Tamayo, Juan Vicente Mendez Mendez
Summary: Super-resolution microscopy has the potential to revolutionize food science by providing images beyond the diffraction limit of light microscopes. However, its application in this field is relatively unknown. This review provides a comprehensive overview of the techniques, advances, and challenges of super-resolution microscopy in food science.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Review
Nanoscience & Nanotechnology
A. Femius Koenderink, Roman Tsukanov, Joerg Enderlein, Ignacio Izeddin, Valentina Krachmalnicoff
Summary: This article summarizes the recent advances in the field of nanophotonics that have leveraged SMLM and shows how some concepts commonly used in nanophotonics can benefit the development of new microscopy techniques for biophysics. It introduces the basic concepts of SMLM and the observables that can be measured, linking them with their corresponding physical quantities of interest in biophysics and nanophotonics.
Article
Chemistry, Multidisciplinary
Shikha Dhiman, Teodora Andrian, Beatriz Santiago Gonzalez, Marrit M. E. Tholen, Yuyang Wang, Lorenzo Albertazzi
Summary: Characterization of newly synthesized materials is crucial in chemistry and nanotechnology, with standardized analytical techniques and novel technologies continually developing. The adoption of reporting standards like MIRIBEL aims to provide reproducible and reliable sample preparation for the scientific community. Super-resolution microscopy (SRM) shows potential to transition into a standard routine method for material characterization, but faces challenges in sample preparation, data analysis, and expensive instrumentation.
Article
Optics
Prakash Joshi, Partha Pratim Mondal
Summary: Molecular assembly in cellular environments is crucial for understanding biological mechanisms. Biophysical parameters directly linked to cellular states require a multimodel approach for accurate estimation.
Article
Plant Sciences
Miroslav Ovecka, Jiri Sojka, Michaela Ticha, George Komis, Jasim Basheer, Cintia Marchetti, Olga Samajova, Lenka Kubenova, Jozef Samaj
Summary: The documentation of plant growth and development requires integrative and scalable approaches. Recent developments in mesoscopy, microscopy and nanoscopy methods have been applied to imaging of plant subcellular compartments, cells, tissues, and organs. However, the current LSFM and SRM modalities have limitations and there is a trend towards designing integrated microscopic modalities for broader multiscale plant imaging.
Article
Chemistry, Physical
Yuting Miao, Robert C. Boutelle, Anastasia Blake, Vigneshwaran Chandrasekaran, Chris J. Sheehan, Jennifer Hollingsworth, Daniel Neuhauser, Shimon Weiss
Summary: Plasmonic nano-objects have great potential in various applications. This study investigates the interaction between quantum dots (QDs) and L-shaped gold nanoantennas, and demonstrates that this strong interaction can induce polarization-dependent modifications to the QD emission intensity, polarization, and localization.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Materials Science, Multidisciplinary
Jiangnan Yuan, Shenfei Zong, Yun Liu, Zhuyuan Wang, Yiping Cui
Summary: The article presents a blinking nanoprobe based on ultra-small silica nanospheres, which has stable chemical properties, excellent fluorescence switching behavior, and better localization precision, suitable for single molecule localization imaging.
Review
Chemistry, Multidisciplinary
Alan M. Szalai, Cecilia Zaza, Fernando D. Stefani
Summary: The combination of super-resolution fluorescence microscopy and FRET has provided unique tools for studying biological systems and nanomaterials. The integration of these techniques has advanced super-resolution imaging and allowed for visualization of molecular interaction patterns with higher spatial resolution. Future perspectives include possible integration with next generation super-resolution techniques for true molecular-scale spatial resolution.
Article
Chemistry, Multidisciplinary
Zhiwei Ye, Wei Yang, Ying Zheng, Shujing Wang, Xiaodong Zhang, Haibo Yu, Shuangshuang Li, Chunxiong Luo, Xiaojun Peng, Yi Xiao
Summary: In this study, a new membrane probe and microfluidic platform were developed to enable synchronous use of super-resolution imaging and single-molecule tracking. This method allows high-throughput observation and analysis of the ultrastructural and dynamic details of living red blood cell membranes, providing new perspectives for future disease diagnostics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Weisong Zhao, Shiqun Zhao, Liuju Li, Xiaoshuai Huang, Shijia Xing, Yulin Zhang, Guohua Qiu, Zhenqian Han, Yingxu Shang, De-En Sun, Chunyan Shan, Runlong Wu, Lusheng Gu, Shuwen Zhang, Riwang Chen, Jian Xiao, Yanquan Mo, Jianyong Wang, Wei Ji, Xing Chen, Baoquan Ding, Yanmei Liu, Heng Mao, Bao-Liang Song, Jiubin Tan, Jian Liu, Haoyu Li, Liangyi Chen
Summary: Sparse structured illumination microscopy (Sparse-SIM) achieves nearly twofold resolution enhancement by utilizing sparse deconvolution algorithm, enabling the resolution of intricate biological structures such as small fusion pores, nuclear pores, and relative movements of inner and outer mitochondrial membranes.
NATURE BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Biagio Mandracchia, Jeonghwan Son, Shu Jia
Summary: The study introduced an optofluidic scanning microscopy (OSM) system for super-resolution, live-cell imaging, utilizing multi-focal excitation through the innate fluidic motion of specimens to achieve effective resolution doubling, optical sectioning, and contrast enhancement. The system features minimal instrumental complexity and full compatibility with various microfluidic configurations, offering a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.
Article
Optics
Oumeng Zhang, Matthew D. Lew
Summary: This study compares the performance of multiple state-of-the-art methods for orientation localization microscopy and identifies optimal imaging methods for various experiment conditions and sample geometries. Additionally, simple modifications to standard fluorescence microscopes exhibit superior performance in many imaging scenarios.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)
Article
Optics
Oumeng Zhang, Matthew D. Lew
Summary: This study proposes a mathematical framework to derive the best possible precision for measuring the position and orientation of dipole-like emitters in any fixed imaging system. It is found that designing an instrument to achieve the maximum sensitivity limit for measuring all possible rotational motions is impossible. Additionally, the best quantum-limited localization precision is shown to be 4%-8% worse than suggested by a scalar monopole model through a vectorial dipole imaging model.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)
Biographical-Item
Chemistry, Physical
Julie S. Biteen, Matthew D. Lew, Katherine A. Willets
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Oumeng Zhang, Weiyan Zhou, Jin Lu, Tingting Wu, Matthew D. Lew
Summary: We present a radially and azimuthally polarized microscope that achieves high detection and estimation performance in single-molecule orientation-localization microscopy (SMOLM). This microscope allows us to measure the localization, orientation, and rotational wobble of Nile red (NR) within supported lipid bilayers (SLBs). The imaging results reveal the presence of randomly oriented binding pockets and cholesterol-rich nanodomains within the SLBs.
Article
Optics
Tingting Wu, Jin Lu, Matthew D. Lew
Summary: This article presents the pixOL algorithm, which optimizes the pixels within a phase mask to produce an engineered Green's function, enabling simultaneous measurement of the 3D orientation and location of a single molecule. The results demonstrate high precision in both experiments and simulations.
Article
Chemistry, Multidisciplinary
Meikun Shen, Tianben Ding, Che Tan, William H. Rackers, Dongyan Zhang, Matthew D. Lew, Bryce Sadtler
Summary: By controlling the surface-ligand redox chemistry on nanowires, the concentration of active sites on photocatalysts can be enhanced, leading to a significant improvement in photocatalytic water oxidation activity. This research result is of great significance for understanding the role of ligands in the catalytic process of nanocrystal catalysts.
Article
Optics
Tingting Wu, Peng Lu, Md Ashequr Rahman, Xiao LI, Matthew D. Lew
Summary: Dipole-spread function (DSF) engineering can reshape microscope images to maximize the sensitivity of measuring the 3D orientations of dipole-like emitters. However, Poisson shot noise, overlapping images, and fitting high-dimensional information pose challenges in image analysis for single-molecule orientation-localization microscopy (SMOLM). In this study, a deep-learning based estimator called Deep-SMOLM is introduced, which achieves superior precision in measuring 3D orientation and 2D position, approaching the theoretical limit. Deep-SMOLM also demonstrates excellent performance in estimating overlapping images of emitters.
Article
Optics
James M. Jusuf, Matthew D. Lew
Summary: This study introduces a simple heuristic for designing a point spread function (PSF) for precise measurement of the distance between two emitters and identifies two types of PSFs that achieve high performance in 3D emitter resolution.
Article
Optics
Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael D. Vahey, Matthew D. Lew
Summary: Single-molecule orientation-localization microscopy is a powerful tool for studying biochemical processes, but the limited photon budget poses challenges for high-dimensional imaging. A raMVR microscope has been developed with isotropic 3D spatial resolution and precision in measuring molecular orientations, enabling detailed imaging of molecular dynamics within biological and chemical systems.
Meeting Abstract
Biophysics
Brian Sun, Weiyan Zhou, Tianben Ding, Tara S. Porter, Tingting Wu, Matthew D. Lew
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Tingting Wu, Matthew R. King, Mina Farag, Rohit V. Pappu, Matthew D. Lew
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael Vahey, Matthew D. Lew
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Tianben Ding, Matthew D. Lew
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Tingting Wu, Jin Lu, Matthew R. King, Rohit V. Pappu, Matthew D. Lew
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Oumeng Zhang, Weiyan Zhou, Matthew D. Lew
BIOPHYSICAL JOURNAL
(2022)