期刊
NANO LETTERS
卷 14, 期 3, 页码 1627-1633出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl500092j
关键词
Dynamic DNA nanotechnology; DNA origami; e-beam lithography; single molecule fluorescence; DNA strand displacement reactions
类别
资金
- Deutsche Forschungsgemeinschaft [SFB 1032 TPA2]
- Deutsche Forschungsgemeinschaft (cluster of excellence Nanosystems Initiative Munich (NIM))
- Volkswagen Stiftung [86 395]
The combination of molecular self-assembly based on the DNA origami technique with lithographic patterning enables the creation of hierarchically ordered nanosystems, in which single molecules are positioned at precise locations on multiple length scales. Based on a hybrid assembly protocol utilizing DNA self-assembly and electron-beam lithography on transparent glass substrates, we here demonstrate a DNA origami microarray, which is compatible with the requirements of single molecule fluorescence and super-resolution microscopy. The spatial arrangement allows for a simple and reliable identification of single molecule events and facilitates automated read-out and data analysis. As a specific application, we utilize the microarray to characterize the performance of DNA strand displacement reactions localized on the DNA origami structures. We find considerable variability within the array, which results both from structural variations and stochastic reaction dynamics prevalent at the single molecule level.
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