Article
Chemistry, Multidisciplinary
Hiromi Takahashi, Yoshinobu Baba, Takao Yasui
Summary: The research focuses on the application of nanowire microfluidics in the analysis of biomolecules, including methods for extraction, separation, and detection, opening up a new academic field in analytical chemistry.
CHEMICAL COMMUNICATIONS
(2021)
Review
Chemistry, Analytical
Li Zhang, Quan-Gen Tan, Jia-Qi Fan, Chen Sun, Yu-Ting Luo, Ru-Ping Liang, Jian-Ding Qiu
Summary: Microfluidic techniques including CE, CEC, and MEKC have been widely used for the chiral separation of biomolecules. Different strategies are employed in these techniques, such as CS-bonded stationary phase in CEC, addition of CS to the mobile phase in CE, and involvement of chiral surfactants and/or CS in pseudostationary phases of MEKC. Microfluidic techniques offer advantages of high selectivity, excellent separation efficiency, and low cost compared to conventional techniques. This review discusses innovations in detection systems, separation mechanism, CS, and chiral analytes of biomolecules, as well as the most applied CSs and the chiral separation of representative biomolecules. Prospects and future developments of microfluidics for chiral separation are also proposed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Kira L. Rahn, Sommer Y. Osman, Quinlan G. Pollak, Robbyn K. Anand
Summary: The COVID-19 pandemic has emphasized the need for sensitive and selective point-of-care diagnostic sensors for early and rapid detection of infection. In this study, ion concentration polarization (ICP) is used to improve the sensitivity of paper-based lateral flow assays (LFAs) by preconcentrating the analyte, SARS-CoV-2 Spike protein (S-protein), directly over a test line. The results show that the described ICP-enhanced LFA can concentrate the S-protein by 21-fold, leading to a significant increase in signal compared to a regular LFA.
ANALYTICAL METHODS
(2023)
Review
Chemistry, Multidisciplinary
Ben Tadgell, Luis M. Liz-Marzan
Summary: Chiral plasmonic nanoparticles interact with biomolecules in distinct ways and can be probed using circular dichroism spectroscopy, offering potential advantages in biosensing and biomedical applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Aurelian John-Herpin, Deepthy Kavungal, Lea von Mucke, Hatice Altug
Summary: This study introduces a deep learning-augmented nanoplasmonic technique that can accurately discriminate between major classes of biomolecules without disrupting native processes.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Analytical
Vesna Bacheva, Federico Paratore, Maya Bar-Dolev, Baruch Rofman, Govind V. Kaigala, Moran Bercovici
Summary: In this article, a microfluidic device for selective separation and extraction of molecules based on their diffusivity is presented. The device utilizes electroosmotically driven bidirectional flows to achieve separation, where high-diffusivity species remain stationary while lower diffusivity species are collected. The device demonstrates high extraction efficiency (>90%) and purity (>99%) over extended periods, making it suitable for processing low sample volumes. Furthermore, the device is shown to be applicable for the extraction of genomic DNA from short DNA fragments.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jian Yan, Changwen Zhao, Yuhong Ma, Wantai Yang
Summary: Compared to traditional 2D biochips, 3D biochips offer higher probe density and detection sensitivity due to their designable surface microstructure and enlarged surface area. In this study, a 3D protein chip was prepared by depositing a monolayer of functionalized hollow silica nanoparticles (HSNs) on an activated cyclic olefin copolymer (COC) substrate. The fabricated chip showed improved roughness and light transmittance of the COC substrate, and exhibited higher immobilization efficiency and detection capability for immunoglobulin G compared to a 2D epoxide group-modified glass slide. This nanoparticle-based 3D protein microarray fabrication approach has great potential in biorelated detection.
Article
Chemistry, Analytical
Ting Sun, Mengyao Li, Feng Zhao, Lin Liu
Summary: The research proposed a one-step, real-time method for the design of SPR biosensors by integrating magnetic preconcentration and separation, which is valuable for the detection of small molecules and ultra-low concentrations of analytes.
Article
Chemistry, Multidisciplinary
Juyoung Son, Yongwon Jung
Summary: In this study, stable protocells with an uptake ability for outside biomolecules were successfully constructed using protein-based liquid condensates and a lipid coating. These protocells, which have high condensate rigidity and specific protein-lipid interactions, can selectively uptake various outside proteins through specific protein interactions.
Article
Polymer Science
Amruta Mutalikdesai, Sudhakar Pagidi, Alfred Hassner, Aharon Gedanken
Summary: This review highlights the recent progress in the enantioselective separation of small molecules using biomolecules/macromolecules with natural inherent chiral moieties, particularly focusing on the significance of biomolecule-based microspheres in terms of green synthesis, selective separation, and potential mechanism for enantiomeric separation of amino acids, drugs, and other racemates.
EUROPEAN POLYMER JOURNAL
(2021)
Review
Biotechnology & Applied Microbiology
Meena Nemiwal, Tian C. Zhang, Dinesh Kumar
Summary: Biosensors have emerged as important tools for selective and sensitive detection of biomolecules. Enzymes are widely used as recognition sites in biosensors, but their immobilization remains challenging. Researchers are exploring the use of functionalized nanomaterials/nanoparticles to enhance the performance of biosensors.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Luca Capua, Yann Sprunger, H. Elettro, F. Risch, A. Grammoustianou, R. Midahuen, T. Ernst, S. Barraud, R. Gill, A. M. Ionescu
Summary: We present a CMOS-compatible double gate and label-free C-reactive protein (CRP) sensor based on silicon on insulator (SOI) silicon nanowires arrays. By exploiting a reference subtracted detection method and a super-Nernstian internal amplification provided by the double gate structure, we overcome the Debye screening of charged CRP proteins in solutions. The demonstrated internal amplification enables the pH response of the sensor, making it an excellent candidate for continuous monitoring of inflammation biomarkers in serum or interstitial fluid.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2022)
Article
Biochemical Research Methods
Xiaojun Luo, Weiling Yue, Shutong Zhang, Haopeng Liu, Zhinan Chen, Ling Qiao, Caijun Wu, Panjie Li, Yi He
Summary: Long-range surface-enhanced Raman scattering (LR-SERS) substrates, based on long-range surface plasmon resonance (LRSPR) configuration, provide enhanced electromagnetic field and deeper penetration depth for the identification of biomacromolecules. The LRSPR mode can be excited and maximized by adjusting the polarizations and geometrical parameters of the LR-SERS substrate. The optimized LR-SERS substrate exhibits ultralow detection limits and excellent selectivity for the diagnosis of SARS-CoV-2 proteins in real samples.
Article
Chemistry, Multidisciplinary
Zhaojun Zhang, Nils Lamers, Chen Sun, Crispin Hetherington, Ivan G. Scheblykin, Jesper Wallentin
Summary: This study presents a low-temperature solution process to fabricate free-standing vertically aligned metal halide perovskite nanowires with excellent optical and electrical properties. Blue-green heterostructured nanowire arrays were also successfully demonstrated. The scalable and high-quality fabrication method offers new possibilities for optoelectronic applications.
Article
Chemistry, Multidisciplinary
Luc Piraux, Nicolas Marchal, Pascal Van Velthem, Tristan da Camara Santa Clara Gomes, Etienne Ferain, Jean-Paul Issi, Vlad-Andrei Antohe
Summary: This paper presents the preparation and characterization of nanocomposite films made from three-dimensional networks of polycrystalline bismuth nanowires. The films were fabricated using electrodeposition within polycarbonate templates, resulting in self-supported networks of crossed nanowires with varying diameter values. The temperature-dependent electrical resistance and thermopower were studied, revealing a decrease in thermopower at smaller diameters due to the contribution of surface charge carriers. Transverse thermoelectricity based on the Nernst effect was also demonstrated on a network with a diameter of 230 nm. These hierarchical architectures offer a reliable solution for flexible thermoelectric devices.
Article
Materials Science, Multidisciplinary
Esther Lestrell, Fernando Patolsky, Nicolas H. Voelcker, Roey Elnathan
Article
Chemistry, Multidisciplinary
Vadim Krivitsky, Marina Zverzhinetsky, Fernando Patolsky
Article
Chemistry, Multidisciplinary
Nimrod Harpak, Guy Davidi, Yarden Melamed, Adam Cohen, Fernando Patolsky
Review
Materials Science, Multidisciplinary
Hashim Alhmoud, Daniel Brodoceanu, Roey Elnathan, Tobias Kraus, Nicolas H. Voelcker
Summary: Metal-assisted chemical etching (MACE) offers control over size, shape, and porosity of porous silicon nanostructures in a single step, making them suitable as biomaterials. The simplicity and flexibility of MACE are advantages, but the complexity of reaction parameters and lack of a complete model hinder rational design. Current understanding of the MACE reaction mechanism still contains uncertainties.
PROGRESS IN MATERIALS SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Yaping Chen, Stella Aslanoglou, Takahide Murayama, Gediminas Gervinskas, Laura I. Fitzgerald, Sharath Sriram, Jie Tian, Angus P. R. Johnston, Yasuhiro Morikawa, Koukou Suu, Roey Elnathan, Nicolas H. Voelcker
ADVANCED MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Yaping Chen, Ji Wang, Xiangling Li, Ning Hu, Nicolas H. Voelcker, Xi Xie, Roey Elnathan
ADVANCED MATERIALS
(2020)
Article
Biotechnology & Applied Microbiology
Roey Elnathan, Andrew W. Holle, Jennifer Young, Marina A. George, Omri Heifler, Andriy Goychuk, Erwin Frey, Ralf Kemkemer, Joachim P. Spatz, Alon Kosloff, Fernando Patolsky, Nicolas H. Voelcker
Summary: Programmable nano-bio interfaces with tuneable vertically configured nanostructures are powerful tools for cellular manipulations and interrogations. However, the opaque nature of many nanostructured surfaces makes non-destructive, live-cell characterization challenging.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
James Carthew, Hazem H. Abdelmaksoud, Margeaux Hodgson-Garms, Stella Aslanoglou, Sara Ghavamian, Roey Elnathan, Joachim P. Spatz, Jurgen Brugger, Helmut Thissen, Nicolas H. Voelcker, Victor J. Cadarso, Jessica E. Frith
Summary: Cells are able to perceive complex mechanical cues from their microenvironment, which influences their development. This study shows that substrate microtopography can modulate nuclear mechanotransduction, alter chromatin organisation, and impact cell fate, especially in MSC osteogenesis. The findings have implications for developing microstructured cell culture substrates for therapeutic applications.
Article
Nanoscience & Nanotechnology
Roey Elnathan, Andy Tay, Nicolas H. Voelcker, Ciro Chiappini
NATURE NANOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Esther Lestrell, Yaping Chen, Stella Aslanoglou, Carmel M. O'Brien, Roey Elnathan, Nicolas H. Voelcker
Summary: Cell nuclear size and shape are regulated and abnormalities can lead to disease. Silicon nanoneedle arrays can modify cell's external environment to study mechanotransduction and intracellular signaling. Our study shows that cell responses to these arrays resemble three-dimensional environments and significantly impact nuclear shape.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Ali-Reza Shokouhi, Yaping Chen, Hao Zhe Yoh, Jason Brenker, Tuncay Alan, Takahide Murayama, Koukou Suu, Yasuhiro Morikawa, Nicolas H. Voelcker, Roey Elnathan
Summary: A non-viral electroactive nanoinjection (ENI) platform is developed to efficiently deliver and express CAR genes in primary human T cells, with high transfection efficiency and minimal cellular perturbation. The ENI-transfected CAR-T cells show effective suppression of lymphoma cell growth, demonstrating the platform's potential for ex vivo cell engineering in CAR-T cell therapy.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Hao Zhe Yoh, Yaping Chen, Ali-Reza Shokouhi, Helmut Thissen, Nicolas H. Voelcker, Roey Elnathan
Summary: The advancement of nanofabrication technologies has enabled the exploration of new territories in nanoneedles applications, particularly in intracellular delivery. In this study, programmable polymeric nanotubes (NTs) were fabricated for efficient mRNA delivery into cells, and the role of actin cytoskeleton in this process was investigated.
Article
Materials Science, Multidisciplinary
James W. Maina, Jennifer M. Pringle, Joselito M. Razal, Stella Aslanoglou, Roey Elnathan, Nicolas H. Voelcker, Ludovic F. Dumee
Summary: Metal hollow nanostructures have great potential in energy storage and conversion due to their unique characteristics. However, fabricating such nanostructures has always been a challenge. This study presents a universal templating strategy for fabricating various metal hollow nanostructures. The electrocatalytic activity of these nanostructures for CO2 conversion is enhanced, demonstrating their effectiveness in energy storage and conversion applications.
MATERIALS ADVANCES
(2022)
Review
Engineering, Biomedical
Yaping Chen, Maria Alba, Terence Tieu, Ziqiu Tong, Rajpreet Singh Minhas, David Rudd, Nicolas H. Voelcker, Anna Cifuentes-Rius, Roey Elnathan
Summary: Different types of engineered nano-bio interfaces, driven by various micro/nanoneedles, nanoparticles, organ-on-chip devices, and nanosubstrates, are advancing scientific and technological progress in biomedical research.
ADVANCED NANOBIOMED RESEARCH
(2021)
Article
Electrochemistry
Dan Schneier, Nimrod Harpak, Svetlana Menkin, Guy Davidi, Meital Goor, Edna Mados, Gilat Ardel, Fernando Patolsky, Diana Golodnitsky, Emanuel Peled
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2020)