期刊
NANO LETTERS
卷 12, 期 7, 页码 3861-3866出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl302069q
关键词
Super-resolution; optical DNA mapping; genomic variations; copy-number variations; SHRImP; SHREC; DNA
类别
资金
- NIH [068625, R01-HG005946]
- NSF [DBI-02-15869, 082265]
- Network for Computational Nanotechnology at Illinois
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1063188] Funding Source: National Science Foundation
Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
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