Article
Microbiology
Christopher J. Day, Alvin W. Lo, Lauren E. Hartley-Tassell, M. Pilar Argente, Jessica Poole, Nathan P. King, Joe Tiralongo, Michael P. Jennings, Mark A. Schembri
Summary: This study focused on dissecting the receptor specificity of different CU fimbriae in Escherichia coli using a glycan array analysis approach with whole-bacterial-cell surface plasmon resonance. The research revealed new information on fimbrial specificity and provided a rapid and scalable system to define novel adhesin-glycan interactions underlying bacterial colonization and disease. The findings contribute to a better understanding of bacterial adhesion mechanisms and offer a promising strategy to combat antibiotic resistance through blocking adhesion.
Article
Food Science & Technology
Alessia Cossettini, Jasmina Vidic, Michela Maifreni, Marilena Marino, Debora Pinamonti, Marisa Manzano
Summary: Listeria, Campylobacter, Salmonella, and Escherichia coli are common causes of foodborne disease in humans, making early and rapid detection crucial for food safety. Recently reported biosensors have shown superior performance in terms of speed, selectivity, sensitivity, and affordability compared to conventional microbiological methods for detecting these bacteria in food.
Review
Chemistry, Multidisciplinary
Zeyu Zhao, Zhiyuan Tian, Feng Yan
Summary: Flexible organic bioelectronic devices are capable of extracting electronic signals from living systems for sensing, recording, and monitoring physiological states. Organic electrochemical transistors (OECTs) have emerged as a promising platform due to their amplification function, high sensitivity, low cost, easy operation, and compatibility with flexible and wearable devices. This review provides a comprehensive overview of recent advancements in flexible OECTs for biosensing applications, covering principles, materials, functionalization, signal acquisition, bioinformatics analysis, and biomimetic devices.
CELL REPORTS PHYSICAL SCIENCE
(2023)
Article
Biophysics
Nirul Masurkar, Naresh Kumar Thangavel, Sally Yurgelevic, Sundeep Varma, Gregory W. Auner, Leela Mohana Reddy Arava
Summary: The fabrication of nano-spacing electrodes through self-assembly technique enables suspended 2D-MoS2 biosensors with enhanced sensitivity and stability, showing promising potential for biomolecule detection including COVID-19 viruses.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biology
Gita Naseri, Hannah Raasch, Emmanuelle Charpentier, Marc Erhardt
Summary: In this study, a set of arabinose-inducible artificial transcription factors (ATFs) were developed using CRISPR/dCas9 and Arabidopsis-derived DNA-binding proteins to control gene expression in E. coli and Salmonella over a wide range of inducer concentrations. The transcriptional output of the ATFs varied greatly, particularly in Salmonella strains rewired for arabinose catabolism. The developed ATFs were used to engineer a Salmonella biosensor strain and regulate beta-carotene biosynthesis in E. coli, resulting in significantly higher production compared to traditional methods.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Huanmin Du, Jinfang Qiao, Yuting Qi, Lingcong Li, Ning Xu, Li Shao, Liang Wei, Jun Liu
Summary: Synthetic biology is used to efficiently produce valuable chemicals by reprogramming the cellular metabolic network. This study developed a H2S2-responsive genetic circuit to reconstruct the l-cysteine biosynthesis network of Corynebacterium glutamicum, resulting in high production of l-cysteine.
Article
Biophysics
Danny Hsu Ko, David Bates, Hande Karaosmanoglu, Karl Taredun, Clare Elton, Leonie Jones, Ali Hosseini, Ashton Partridge
Summary: The increased sensitivity of microelectrode arrays (MEAs) over macroelectrodes for biosensing is due to the reduced diffusion gradient of the target species to and from the electrode surfaces. A polymer-based 3D MEA exhibits higher sensitivity by enhancing the diffusion profile of the target species and inducing differential current distribution focused at the apices of individual electrodes. The electrochemical characteristics of the 3D MEAs show ideal microelectrode behavior with three orders of magnitude greater sensitivity than ELISA.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Environmental Sciences
Alana Marie de Cezaro, Sandra Cristina Ballen, Lucelia Hoehne, Juliana Steffens, Clarice Steffens
Summary: This paper reviews the detection of endocrine disruptors in water, discussing various methods such as chromatography, immunoassays, and different types of biosensors. Cantilever-based biosensors have shown interesting results in detecting specific substances, especially endocrine disruptors in water.
WATER AIR AND SOIL POLLUTION
(2021)
Article
Materials Science, Biomaterials
Lauren Gwynne, George T. Williams, Kai-Cheng Yan, Bethany L. Patenall, Jordan E. Gardiner, Xiao-Peng He, Jean-Yves Maillard, Tony D. James, Adam. C. Sedgwick, A. Toby. A. Jenkins
Summary: Alkaline phosphatase (ALP) is an important enzyme biomarker with potential in detecting pathogenic bacteria. Research has shown that ALP has colorimetric and fluorescent responses towards Staphylococcus aureus and demonstrates selectivity over other bacteria. Encapsulation of ALP in hydrogels can be used as smart wound dressings for monitoring and treating wound infections.
BIOMATERIALS SCIENCE
(2021)
Review
Biochemical Research Methods
Arash Mohammadinejad, Ghazaleh Aleyaghoob, Samin Nooranian, Lorena Dima, Marius Alexandru Moga, Mihaela Badea
Summary: Fibrinogen, a major inflammation marker and blood coagulation factor, plays a vital role in human health. Fast, simple, easy to use, and inexpensive methods for monitoring fibrinogen are in high demand. Biosensors have gained attention for their ability to perform analyses at the point-of-care using self-testing devices.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Antonio Alessio Leonardi, Maria Jose Lo Faro, Alessia Irrera
Summary: Biosensors are revolutionizing healthcare systems worldwide by enabling the early detection of various diseases. Silicon, as a leader in the microelectronics industry, may bring about significant changes in healthcare through novel sensors based on this material. These sensors show promising potential for industrial transfer and have advantages in terms of sensitivity and selectivity for medical device applications.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Mehmet Cagri Soylu, Sukru Taner Azgin
Summary: This study explored a method using sewage sludge and cow dung to produce organic fertilizer, with a unique QCM detection system for low-level detection of E. coli in vermicompost tea. The results showed that sewage sludge could be a valuable liquid organic fertilizer, and the QCM biosensing demonstrated good detection sensitivity and stability for sensitive measurement of E. coli.
Article
Chemistry, Multidisciplinary
Martin Lee, Martin P. Robin, Ruben H. Guis, Ulderico Filippozzi, Dong Hoon Shin, Thierry C. van Thiel, Stijn P. Paardekooper, Johannes R. Renshof, Herre S. J. van der Zant, Andrea D. Caviglia, Gerard J. Verbiest, Peter G. Steeneken
Summary: This work demonstrates the use of self-sealing membranes to seal the reference cavity beneath, improving the gas permeation time constant. The adhesion increase over SiO2 is mediated by oxygen bonds that are formed at the SiO2/complex oxide interface during the self-sealing anneal.
Article
Physics, Multidisciplinary
Samira Jalilvand, Reza Sepahvand, Hamze Mousavi
Summary: The electronic properties of DNA and RNA nanowires were investigated using the tight-binding model and the Green's function formalism. Increasing the number of sites in the unit cells reduced the influence of randomness on the electronic properties, and the sugar-phosphate backbone created bandgaps in the band structure and density of states. Increasing the number of bases suppressed the effect of damage, with a more pronounced effect observed for nonzero on-site energies.
EUROPEAN PHYSICAL JOURNAL PLUS
(2022)
Article
Electrochemistry
Miaosi Li, Chathurika Abeyrathne, Daniel P. Langley, Luke R. Cossins, Anushka N. Samudra, George W. Green, Simon E. Moulton, Saimon M. Silva
Summary: Electrochemical glycoprotein sensors are an effective tool for cancer detection, therapeutic design, and monitoring. The combination of lubricin (LUB) and peanut agglutinin (PNA) has been used to directly detect tumor-associated short glycans in bodily fluids. This study extends the use of the LUB-PNA interface to monitor glycan binding interactions using redox species in solution. By measuring cyclic voltammetry and electrochemical impedance spectroscopy, the study reveals the altered behavior of ferrocyanide redox species in the presence of cancer-associated T-antigen glycoprotein. The sensor also enables the detection of ASF in human blood.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Philip Boehm, Alexandros Koutsioubas, Jean-Francois Moulin, Joachim O. Raedler, Erich Sackmann, Bert Nickel
Article
Chemistry, Multidisciplinary
David Volbers, Valentin K. Stierle, Konstantin J. Ditzel, Julian Aschauer, Joachim O. Raedler, Madeleine Opitz, Philipp Paulitschke
Article
Biochemistry & Molecular Biology
Stefanie Kiderlen, Christoph Polzer, Joachim O. Raedler, Denitsa Docheva, Hauke Clausen-Schaumann, Stefanie Sudhop
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Physics, Multidisciplinary
David B. Brueckner, Alexandra Fink, Christoph Schreiber, Peter J. F. Roettgermann, Joachim O. Raedler, Chase P. Broedersz
Correction
Physics, Multidisciplinary
David B. Bruckner, Alexandra Fink, Christoph Schreiber, Peter J. F. Rottgermann, Joachim O. Radler, Chase P. Broedersz
Article
Biochemical Research Methods
Christoph Polzer, Stefan Ness, Mojtaba Mohseni, Thomas Kellerer, Markus Hilleringmann, Joachim Raedler, Thomas Hellerer
BIOMEDICAL OPTICS EXPRESS
(2019)
Article
Multidisciplinary Sciences
Fang Zhou, Sophia A. Schaffer, Christoph Schreiber, Felix J. Segerer, Andriy Goychuk, Erwin Frey, Joachim O. Raedler
Article
Multidisciplinary Sciences
Christoph Schreiber, Behnam Amiri, Johannes C. J. Heyn, Joachim O. Raedler, Martin Falcke
Summary: The study explored the motion of cells under different Fibronectin densities and revealed the regulatory roles of adhesion and velocity in cell velocity, as well as the relationship between leading-edge and rear adhesion structures. The findings provide a quantitative mechanistic understanding of the adhesion-velocity relation and cell response to changes in adhesion, integrating various factors such as force-dependent polymerization, retrograde flow, positive feedback, and spring-like front-rear interaction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
David B. Brueckner, Nicolas Arlt, Alexandra Fink, Pierre Ronceray, Joachim O. Raedler, Chase P. Broedersz
Summary: The study found that the interactions between noncancerous cells are mainly described by repulsion and friction interactions, while cancerous cells exhibit attraction and antifriction interactions. These interaction behaviors were validated in experiments and may provide a unifying theoretical description for diverse cellular interaction behaviors of different cell types.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Instruments & Instrumentation
Tamara Ehm, Julian Philipp, Martin Barkey, Martina Ober, Achim Theo Brinkop, David Simml, Miriam von Westphalen, Bert Nickel, Roy Beck, Joachim O. Raedler
Summary: This paper presents a 3D-printed X-ray chamber that allows for in situ exchange of buffer and in situ optical transmission spectroscopy. The chamber is capable of measuring reversible structural changes in samples under different conditions and enables the study of responsive materials through combined microfluidic exchange and optical spectroscopy.
JOURNAL OF SYNCHROTRON RADIATION
(2022)
Article
Chemistry, Physical
Miriam Grava, Mohd Ibrahim, Akhil Sudarsan, Julio Pusterla, Julian Philipp, Joachim O. Raedler, Nadine Schwierz, Emanuel Schneck
Summary: The protonation degree and lipid packing of ionizable lipids play crucial roles in the performance of lipid-based nanoparticles, and a combination of molecular dynamics simulations and experimental measurements can provide valuable insights for further predictive modeling.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Biochemical Research Methods
Stefan Stoeberl, Miriam Balles, Thomas Kellerer, Joachim O. O. Raedler
Summary: Researchers introduced RGD-functionalized hydrogel structures with pressurized clefts for evaluating invasive migration of cells. Equally spaced blocks of PEG-NB hydrogels were formed using UV-photolithography, which swelled and closed the interjacent gaps, enabling the study of cancer cell transmigration through these clefts. This approach provides soft 3D-microstructures mimicking invasion conditions in the extracellular matrix.
Article
Physics, Fluids & Plasmas
Bernhard G. Mitterwallner, Christoph Schreiber, Jan O. Daldrop, Joachim O. Raedler, Roland R. Netz
Article
Biology
Alexandra Murschhauser, Peter J. F. Roettgermann, Daniel Woschee, Martina F. Ober, Yan Yan, Kenneth A. Dawson, Joachim O. Raedler
COMMUNICATIONS BIOLOGY
(2019)
Correction
Mathematical & Computational Biology
Fabian Froehlich, Anita Reiser, Laura Fink, Daniel Woschee, Thomas Ligon, Fabian Joachim Theis, Joachim Oskar Raedler, Jan Hasenauer
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2019)
