期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 209, 期 -, 页码 645-651出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.12.040
关键词
VEGF; Aptamer; Cancer biomarker; Biosensor; Capacitance
资金
- Scientific and Technological Research Council of Turkey (TUBITAK) [110E287]
In this paper, we report on in vitro anti-VEGF aptamer selection and the development of a capacitive aptamer-antibody based sandwich assay for sensitive detection of vascular endothelial growth factor165 (VEGF) in human serum. The assay design involves capturing of VEGF protein through two epitopes, one with anti-VEGF aptamer and the other with the antibody. The capacitive sensor was functionalized with anti-VEGF aptamer which first captures the VEGF protein followed by sandwiching with antibody conjugated magnetic beads (MB-Abs). Changes in capacitance was measured using non-Faradaic electrochemical impedance spectroscopy (nFIS) at different AC electrical frequencies (50-300 MHz). The sandwich assay format exhibited enhanced capacitive signal with respect to concentration with a detection range 5 pg mL(-1) to 1 ngmL(-1) of VEGF protein in human serum. Use of anti-VEGF aptamers enabled chemical stability on sensors during and after the chemical coupling on sensors. This work demonstrated the successful application of on-chip aptamer-antibody based sandwich assays for detection of target proteins in real serum sample for early cancer diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据