期刊
MYCOSES
卷 56, 期 6, 页码 672-680出版社
WILEY-BLACKWELL
DOI: 10.1111/myc.12093
关键词
Silver nanoparticles; nystatin; chlorhexidine digluconate; synergism; Candida biofilms
资金
- CAPES [BEX 1221/10-8]
- FAPESP, Brazil [2009/15146-5]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [09/15146-5] Funding Source: FAPESP
Although silver nanoparticles (SN) have been investigated as an alternative to conventional antifungal drugs in the control of Candida-associated denture stomatitis, the antifungal activity of SN in combination with antifungal drugs against Candida biofilms remains unknown. Therefore, the aim of this study was to evaluate the antifungal efficacy of SN in combination with nystatin (NYT) or chlorhexidine digluconate (CHG) against Candida albicans and Candida glabrata biofilms. The drugs alone or combined with SN were applied on mature Candida biofilms (48h), and after 24h of treatment their antibiofilm activities were assessed by total biomass quantification (by crystal violet staining) and colony forming units enumeration. The structure of Candida biofilms was analysed by scanning electron microscopy (SEM) images. The data indicated that SN combined with either NYT or CHG demonstrated synergistic antibiofilm activity, and this activity was dependent on the species and on the drug concentrations used. SEM images showed that some drug combinations were able to disrupt Candida biofilms. The results of this study suggest that the combination of SN with NYT or CHG may have clinical implications in the treatment of denture stomatitis. However, further studies are needed before recommending the use of these drugs safely in clinical situations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据