Article
Pharmacology & Pharmacy
Lin Cheng, Zaiming Liang, Fang Liu, Ling Lin, Jiao Zhang, Linli Xie, Mingjie Yu, Fengjun Sun
Summary: This study aimed to investigate the independent factors influencing the concentration of voriconazole (VCZ) in order to optimize dosing regimens and maintain therapeutic concentrations. It was found that in younger adult patients, the levels of total bile acid (TBA), glutamic-pyruvic transaminase (ALT), and the use of proton-pump inhibitors were independent factors influencing VCZ concentration. In elderly patients, direct bilirubin (DBIL), albumin, and estimated glomerular filtration rate (eGFR) were found to be influential factors.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Microbiology
Takeo Yasu, Yuka Nomura, Yoshito Gando, Yasuhiko Matsumoto, Takashi Sugita, Nobuharu Kosugi, Masayuki Kobayashi
Summary: The study aimed to develop an ultra-simple method to measure plasma voriconazole concentration, which will help improve the implementation rate of voriconazole therapeutic drug monitoring and contribute to effective and safe use of the drug.
Article
Pharmacology & Pharmacy
Lin Cheng, Yue Zhao, Zaiming Liang, Xi You, Changsheng Jia, Xiuying Liu, Qian Wang, Fengjun Sun
Summary: This study aimed to develop a machine learning-based method for predicting toxic plasma trough concentration of voriconazole (VCZ). Three machine learning algorithms were used to model data from adult patients, and an external validation set was used to verify the reliability of the models. The results showed that these machine learning models can accurately predict the toxic concentration exposure of VCZ.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Dermatology
Christelle Boglione-Kerrien, Jeff Morcet, Lucie-Marie Scailteux, Francois Benezit, Christophe Camus, Jean-Baptiste Mear, Jean-Pierre Gangneux, Eric Bellissant, Camille Tron, Marie-Clemence Verdier, Florian Lemaitre
Summary: This study aimed to implement routine measurement of voriconazole N-oxide (NOX) concentrations, describe the metabolic ratio (MR), and propose a suggested dosage-adjustment algorithm for therapeutic drug monitoring (TDM) of patients treated with voriconazole (VRC). The MR based on NOX proved to be useful for interpretation and clinical decision-making, especially for complex patients. A dosing algorithm was suggested based on the results.
Article
Pharmacology & Pharmacy
Su-jie Jia, Ke-qin Gao, Pan-hao Huang, Ren Guo, Xiao-cong Zuo, Qing Xia, Shuang-yao Hu, Zhen Yu, Yue-liang Xie
Summary: Glucocorticoids reduced C-min/dose levels of VRC and different SNPs of CYP450 have varied effects on the C-min/dose ratio of VRC. Glucocorticoids and CYP2C19*17 mutants had a synergistic effect in reducing VRC C-min/dose. The clinical efficacy of VRC should be carefully monitored, especially in the presence of CYP2C19*17 mutants when combined with glucocorticoids.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Chiara Cattaneo, Jon Salmanton-Garcia, Francesco Marchesi, Shaimaa El-Ashwah, Federico Itri, Barbora Weinbergerova, Maria Gomes Da Silva, Michelina Dargenio, Julio Davila-Valls, Sonia Martin-Perez, Francesca Farina, Jaap Van Doesum, Toni Valkovic, Caroline Besson, Christian Bjorn Poulsen, Alberto Lopez-Garcia, Pavel Zak, Martin Schonlein, Klara Piukovics, Ozren Jaksic, Alba Cabirta, Natasha Ali, Uluhan Sili, Nicola Fracchiolla, Giulia Dragonetti, Tatjana Adzic-Vukicevic, Monia Marchetti, Marina Machado, Andreas Glenthoj, Olimpia Finizio, Fatih Demirkan, Ola Blennow, Maria Chiara Tisi, Ali S. Omrani, Milan Navratil, Zdenek Racil, Jan Novak, Gabriele Magliano, Moraima Jimenez, Carolina Garcia-Vidal, Nurettin Erben, Maria Ilaria Del Principe, Caterina Buquicchio, Rui Bergantim, Josip Batinic, Murtadha Al-Khabori, Luisa Verga, Tomas Szotkowski, Michail Samarkos, Irati Ormazabal-Velez, Stef Meers, Johan Maertens, Laszlo Imre Pinczes, Martin Hoenigl, L'ubos Drgona, Annarosa Cuccaro, Yavuz M. Bilgin, Avinash Aujayeb, Laman Rahimli, Stefanie Grafe, Mariarita Sciume, Milos Mladenovic, Gokce Melis Colak, Maria Vittoria Sacchi, Anna Nordlander, Caroline Berg Venemyr, Michaela Hanakova, Nicole Garcia-Pouton, Ziad Emarah, Giovanni Paolo Maria Zambrotta, Raquel Nunes Rodrigues, Raul Cordoba, Gustavo-Adolfo Mendez, Monika M. Biernat, Oliver A. Cornely, Livio Pagano
Summary: Patients with simultaneous diagnosis of haematological malignancies (HM) and COVID-19 pose a greater challenge for hematologists. It was found that patients not receiving HM treatment had a higher mortality rate, and age, severe/critical COVID-19, >= 2 comorbidities, and lack of HM treatment were identified as independent risk factors for mortality. These observations highlight the importance of HM treatment in these patients.
Article
Biochemical Research Methods
Zhi-Peng Wen, Hong Zhang, Qin Li, Chu-Jiao Hu, Cheng-Li Yang, Xing-Hua Fan, Zhi-Chang Zheng, Ming Li, Lin Tan, Rong Tan, Bin-Yi Xia, Jing Ji, Yang Bai, Yi Yang, Xiao-Li Zheng, Ji-Hong Yang
Summary: In this study, a reliable, accurate, sensitive, and quick method for determining voriconazole plasma levels was developed and applied to personalized therapy for pediatric patients with hematologic tumors.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Oncology
Lito E. Papanicolas, Sarah K. Sims, Steven L. Taylor, Sophie J. Miller, Christos S. Karapetis, Steve L. Wesselingh, David L. Gordon, Geraint B. Rogers
Summary: Conventional myelosuppressive chemotherapy results in significant changes in gut microbiota characteristics during the predicted myelosuppression period post-chemotherapy, but these changes are no longer significant at the end of the chemotherapy cycle. Further research is needed to link microbiome changes during chemotherapy to clinical outcomes.
Review
Immunology
Cedric Hupperetz, Sangjoon Lah, Hyojin Kim, Chan Hyuk Kim
Summary: Chimeric antigen receptor (CAR) T cell therapy has shown remarkable potential in treating hematological malignancies, but its application in solid tumors faces challenges such as antigen heterogeneity and the immunosuppressive tumor microenvironment. Additionally, CAR T cell therapy holds promise in infectious diseases and autoimmune diseases.
Article
Infectious Diseases
Anette Veringa, Roger J. Bruggemann, Lambert F. R. Span, Bart J. Biemond, Mark G. J. de Boer, Edwin R. van den Heuvel, Saskia K. Klein, Doris Kraemer, Monique C. Minnema, Niek H. J. Prakken, Bart J. A. Rijnders, Jesse J. Swen, Paul E. Verweij, Marielle J. Wondergem, Paula F. Ypma, Nicole Blijlevens, Jos G. W. Kosterink, Tjip S. van der Werf, Jan-Willem C. Alffenaar
Summary: This study investigated whether TDM-guided voriconazole treatment is superior to standard treatment for invasive aspergillosis. The results showed no significant difference in treatment outcome and adverse reactions between the TDM and non-TDM groups, but a higher proportion of voriconazole concentrations within the acceptable range were found in the TDM group.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Hematology
Shuai-Shuai Ge, Qiao-Cheng Qiu, Hai-Ping Dai, Xiang-Dong Shen, Tian-Mei Wu, Jia-Hui Du, Chao-Ling Wan, Hong-Jie Shen, De-Pei Wu, Sheng-Li Xue, Song-Bai Liu
Summary: In this study, the spectrum of FLT3 mutations in haematological malignancies was investigated. Four types of non-canonical FLT3 mutations were identified, including non-canonical point mutations, deletion, frameshift, and ITD outside the JMD and TKD1 regions. The survival of patients with high-frequency FLT3-NCPM in AML was shown to be comparable to those with canonical TKD mutations. Additionally, certain deletion and ITD mutants showed higher kinase activity than wild-type FLT3, and all tested deletion mutations and ITD were sensitive to AC220 and sorafenib.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Review
Hematology
Michael J. Austin, Foteini Kalampalika, William P. Cawthorn, Bela Patel
Summary: While bone marrow adipocytes (BMAd) have been recognized by clinical haemato-pathologists for a long time, biologists have only recently begun to study the role of the adipocytic niche. There is increasing evidence to suggest that BMAds are not just a structural component of bone marrow, but a key player in physiological and pathological processes related to health and disease. Efforts are being made to understand how BMAds contribute to health and disease, with the ultimate goal of developing clinical applications. This review summarizes the emerging role of BMAds in normal and pathological haematological conditions, with a focus on their impact on cancer therapy.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Mingjie Yu, Jun Yang, Lirong Xiong, Shipeng Zhan, Lin Cheng, Yongchuan Chen, Fang Liu
Summary: The correlation and concordance of UPLC-MS/MS and EMIT in determining VRZ plasma concentration were compared in this study. The results showed a high correlation but poor concordance between UPLC-MS/MS and EMIT. Adjusted EMIT results may serve as a reliable substitute when UPLC-MS/MS results cannot be obtained.
Article
Microbiology
Yutaro Hino, Akira Watanabe, Rio Seki, Shokichi Tsukamoto, Yusuke Takeda, Emiko Sakaida, Katsuhiko Kamei
Summary: Invasive fungal disease (IFD) in patients with haematological disorders is a fatal disease that requires rapid identification and treatment. This study aimed to investigate the epidemiology and causative organisms of IFD in Japan, with Aspergillus, Candida, and Fusarium being the major pathogens identified. The study highlighted the importance of detailed identification of the causative fungi for effective therapeutic options.
Article
Infectious Diseases
Yi-Chang Zhao, Yang Zou, Jing-Jing Hou, Chen-Lin Xiao, Bi-Kui Zhang, Jia-Kai Li, Da-Xiong Xiang, Indy Sandaradura, Miao Yan
Summary: The study aimed to explore optimized maintenance dose of voriconazole in children and factors affecting trough concentration. The probability of achieving target concentration increased after adjustment, with maintenance doses varying based on age and weight.
Article
Infectious Diseases
S. Rocchi, E. Scherer, C. Mengoli, A. Alanio, F. Botterel, M. E. Bougnoux, S. Bretagne, M. Cogliati, M. Cornu, F. Dalle, C. Damiani, J. Denis, S. Fuchs, M. Gits-Muselli, F. Hagen, C. Halliday, R. Hare, X. Iriart, C. Klaassen, M. Lackner, M. Lengerova, V Letscher-Bru, F. Morio, C. Nourrisson, W. Posch, B. Sendid, J. Springer, B. Willinger, P. L. White, R. A. Barnes, M. Cruciani, J. P. Donnelly, J. Loeffler, L. Millon
Summary: Interlaboratory evaluations of Mucorales qPCR assays showed good reproducibility and performance, supporting its use as part of the diagnostic strategy for mucormycosis.
Article
Microbiology
Marketa Nykrynova, Vojtech Barton, Karel Sedlar, Matej Bezdicek, Martina Lengerova, Helena Skutkova
Summary: Genotyping methods can be used to distinguish bacterial strains, and the proposed method based on variable genetic segments can help identify transmission routes and sources of highly virulent and multiresistant strains.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Barbora Weinbergerova, Jiri Mayer, Stepan Hrabovsky, Zuzana Novakova, Zdenek Pospisil, Lucie Martykanova, Katerina Hortova, Lucie Mandelova, Karel Hejduk, Renata Chloupkova, Michal Pospisil, Martina Doubkova, Vladimir Marek, Renata Novotna, Martin Dolecek, Hana Matejovska Kubesova, Kristian Brat, Radana Parizkova, Petr Husa, Marek Mechl, Zdenek Kral, Martina Lengerova
Summary: This study aimed to investigate the natural trajectory of COVID-19 in relation to viral load among the Czech population. The research involved 105 outpatients and revealed that most patients developed symptoms around 6 days after onset, with fevers, respiratory issues, and anosmia being the most common complaints. Telemonitoring was effective in tracking symptom progression and detecting complications early on.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Robin Jugas, Karel Sedlar, Martin Vitek, Marketa Nykrynova, Vojtech Barton, Matej Bezdicek, Martina Lengerova, Helena Skutkova
Summary: CNV detection in bacteria is less focused on compared to eukaryotes, but with increasing interest due to challenges in bacterial drug resistance. CNproScan is a bacterial genome CNV detection method that can detect shorter events and provide classification, showing improvements over existing methods.
Letter
Oncology
Barbora Weinbergerova, Jiri Mayer, Tomas Kabut, Stepan Hrabovsky, Jirina Prochazkova, Zdenek Kral, Vladimir Herout, Rita Pacasova, Lenka Zdrazilova-Dubska, Petr Husa, Petr Bednar, Daniel Ruzek, Martina Lengerova
HEMATOLOGICAL ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Matej Bezdicek, Marketa Nykrynova, Karel Sedlar, Stanislava Kralova, Jana Hanslikova, Aja Komprdova, Helena Skutkova, Iva Kocmanova, Jiri Mayer, Martina Lengerova
Summary: Mini-MLST is a rapid, cost-effective, and high-throughput genotyping method with great portability, despite slightly lower discriminatory power compared to other typing methods. It proves to be ideal for screening, subgrouping, and quick strain typing during outbreaks.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Chemical
Karel Sedlar, Marketa Nykrynova, Matej Bezdicek, Barbora Branska, Martina Lengerova, Petra Patakova, Helena Skutkova
Summary: Clostridium beijerinckii, a widely studied bacterium, was analyzed for its genome diversity and evolution, revealing misidentification of certain strains and defining the pangenome of the species. The core genome contains genes for basic metabolism, while the accessory genome affects final phenotype, showing great diversity within the species with potential for biotechnological applications.
Article
Biotechnology & Applied Microbiology
Jana Musilova, Xenie Kourilova, Iva Pernicova, Matej Bezdicek, Martina Lengerova, Stanislav Obruca, Karel Sedlar
Summary: This study provides an in-depth characterization of A. thermoaerophilus CCM 8960, including genome sequencing and assembly, as well as the study of genes involved in PHA metabolism. The bacterium shows promising features that make it a potential candidate for industrial production of PHA.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Editorial Material
Medicine, General & Internal
Jan Stingl, Julie Hylmarova, Martina Lengerova, Jan Malaska, Jan Stasek
Summary: Cryptococcal superinfection is a rare but potentially fatal complication, especially if its detection and subsequent treatment are delayed. This case report highlights the disturbance of the immune system in a patient with no previously known immune suppression, which is consistent with the changes observed in cellular immunity in COVID-19. Despite receiving standard treatment, the patient's condition deteriorated and eventually resulted in death.
Article
Microbiology
Marketa Nykrynova, Roman Jakubicek, Vojtech Barton, Matej Bezdicek, Martina Lengerova, Helena Skutkova
Summary: Nanopore sequencing has become popular due to its rapid and simple library preparation, sequencing versatility, and longer read lengths compared to next-generation sequencing. However, data postprocessing remains a time-consuming and computationally demanding bottleneck. In this study, a neural network-based method is proposed, which can detect and classify specific genomic regions directly from the raw nanopore signals. This eliminates the need for basecalling and allows for real-time squiggle processing.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marketa Nykrynova, Vojtech Barton, Matej Bezdicek, Martina Lengerova, Helena Skutkova
Summary: The study introduces a pipeline for identifying highly variable genomic fragments in unmapped reads through a modified hybrid assembly approach. These variable regions can be used in efficient laboratory methods for bacterial typing with high discriminatory power, such as mini-MLST, replacing expensive methods like MLST. Through this approach, infection monitoring can be carried out more rapidly.
Article
Microbiology
Kristyna Dufkova, Matej Bezdicek, Marketa Nykrynova, Iva Kocmanova, Petra Kubackova, Jana Hanslikova, Karolina Fejkova, Jiri Mayer, Martina Lengerova
Summary: In this study, a novel mini-MLST typing scheme for Pseudomonas aeruginosa was designed. It has great discriminatory power, ease of performance, and short processing time, making it suitable for prospective typing of large isolate sets. Integrating the novel typing scheme enables the investigation of the development and spread of multidrug-resistant/extensively drug-resistant high-risk clones.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Matej Bezdicek, Jana Hanslikova, Marketa Nykrynova, Kristyna Dufkova, Iva Kocmanova, Petra Kubackova, Jiri Mayer, Martina Lengerova
Summary: Enterococcus faecium is a significant pathogen causing healthcare-associated infections, and its resistance to antibiotics complicates treatment. The current MLST scheme does not accurately reflect genetic relatedness between strains. A new MLST scheme based on genome analysis was proposed, which showed higher discriminatory power and identified more clonal complexes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Kristyna Dufkova, Matej Bezdicek, Kristina Cuprova, Dagmar Pantuckova, Marketa Nykrynova, Eva Brhelova, Iva Kocmanova, Silvie Hodova, Marketa Hanslianova, Tomas Juren, Bretislav Lipovy, Jiri Mayer, Martina Lengerova
Summary: In this study, a novel molecular typing approach for Staphylococcus aureus was established, combining high-resolution melting analysis and multilocus sequence typing. This approach is rapid, reproducible, and cost-efficient, and has great potential for improving epidemiological investigations of S. aureus.
MICROBIOLOGY SPECTRUM
(2022)
Article
Evolutionary Biology
Jana Musilova, Xenie Kourilova, Matej Bezdicek, Martina Lengerova, Stanislav Obruca, Helena Skutkova, Karel Sedlar
Summary: Schlegelella thermodepolymerans is a moderately thermophilic bacterium capable of producing polyhydroxyalkanoates - biodegradable polymers. The complete genome of the type strain S. thermodepolymerans DSM 15344 was assembled using a hybrid approach, and it revealed a high number of genes with unknown function or unknown clusters of orthologous groups, indicating limited knowledge of thermophilic polyhydroxyalkanoates-producing bacteria at the genome level.
GENOME BIOLOGY AND EVOLUTION
(2021)