Article
Cell Biology
Ludmila Matos Baltazar, Gabriela Fior Ribeiro, Gustavo J. Freitas, Celso Martins Queiroz-Junior, Caio Tavares Fagundes, Carlos Chaves-Olortegui, Mauro Martins Teixeira, Daniele G. Souza
Summary: Paracoccidioidomycosis is a systemic disease endemic in Latin America, with most cases registered in Brazil. Research has been conducted to improve treatment effectiveness and protection against the disease, including the investigation of extracellular vesicles as potential antigen delivery vehicles.
Article
Microbiology
Caroline Maria Marcos, Haroldo Cesar de Oliveira, Patricia Akemi Assato, Lariane Teodoro de Oliveira, Nathalia Fregonezi, Kelvin Sousa dos Santos, Caroline Barcelos Costa-Orlandi, Ana Marisa Fusco-Almeida, Maria Jose Soares Mendes-Giannini
Summary: This study explores signaling pathways implicated in fungal pathobiology and identifies the crucial role of the Drk1 protein in the morphological shift and virulence of Paracoccidioides brasiliensis. Peptides with affinity for PbDrk1 were found to inhibit the phase transition and adhesion of P. brasiliensis, and augment the effects of antifungal agents. The peptides also showed enhanced larval survival rates in a Galleria mellonella model. Further investigation of PbDrk1 may improve our understanding of P. brasiliensis and lead to the development of novel therapeutic strategies.
Article
Immunology
Aparecido Ferreira de Souza, Laurine Lacerda Pigosso, Lana O'Hara Souza Silva, Italo Dany Cavalcante Galo, Juliano Domiraci Paccez, Kleber Santiago Freitas e Silva, Milton Adriano Pelli de Oliveira, Maristela Pereira, Celia Maria de Almeida Soares
Summary: In this study, the exoproteome of Paracoccidioides brasiliensis (Pb18) under iron deprivation conditions was analyzed using nanoUPLC-MSE. One hundred forty-one proteins were identified, out of which 64 were predicted to be secreted. The regulation of several virulence factors was also identified, and Cyb5 was highlighted as a secreted molecule potentially involved in iron homeostasis in P. brasiliensis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Eunbyeol Ko, Soyoung Jeong, Min Yong Jwa, A. Reum Kim, Ye-Eun Ha, Sun Kyung Kim, Sungho Jeong, Ki Bum Ahn, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Summary: The study showed that compared to h-SP or f-SP vaccine, r-SP vaccine can provide higher protection against S. pneumoniae, enhance specific antibody levels, and promote follicular helper T cell activation.
Article
Immunology
Juliana S. de Curcio, Lucas Nojosa Oliveira, Mariana P. Batista, Evandro Novaes, Celia Maria de Almeida Soares
Summary: Research shows that under iron deprivation conditions, Paracoccidioides brasiliensis produces a variety of microRNAs, which may regulate metabolic pathways related to iron deficiency.
MICROBES AND INFECTION
(2021)
Article
Plant Sciences
Lauana Aparecida Santos, Pedro Luiz Rosalen, Nayara Andrade Dias, Julianne Caravita Grisolia, Bruno Jose Nascimento Gomes, Lucas Blosfeld-Lopes, Masaharu Ikegaki, Severino Matias de Alencar, Eva Burger
Summary: Brazilian Red propolis shows direct antifungal activity against Paracoccidioides brasiliensis, activates neutrophils, prevents fungal dissemination, and controls excessive inflammation.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Thyago R. Cardim-Pires, Ricardo Sant'Anna, Debora Foguel
Summary: Studies have shown that the specific epitope P10 of gp43 forms amyloid aggregates in solution, potentially hindering vaccine development. Fragmentation of P10 fibrils can induce the aggregation of P4 peptide, while in silico analysis suggests that other aggregation-prone peptides similar to P10 could be generated under physiological conditions. Based on these findings, the sequence of P10 has been redesigned to reduce its aggregation propensity while maintaining its antigenic region.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Stephan Alberto Machado de Oliveira, Janayna Nunes Reis, Elisa Catao, Andre Correa Amaral, Ana Camila Oliveira Souza, Alice Melo Ribeiro, Lucia Helena Faccioli, Fabiana Pirani Carneiro, Clara Luna Freitas Marina, Pedro Henrique Burgel, Larissa Fernandes, Aldo Henrique Tavares, Anamelia Lorenzetti Bocca
Summary: The results of the study indicate that CD18(low) mice infected with Pb18 survived with fewer granulomas, lower fungal burden, lower nitrate levels, and high IgG1 levels compared to WT mice. In addition, the in vitro macrophages from CD18(low) mice showed slower internalization of yeast cells and lower fungal burden, suggesting that beta 2 integrins play a crucial role in fungal survival within macrophages.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Lucas Nojosa Oliveira, Patricia de Sousa Lima, Danielle Silva Araujo, Igor Godinho Portis, Agenor de Castro Moreira dos Santos Junior, Alexandre Siqueira Guedes Coelho, Marcelo Valle de Sousa, Carlos Andre Ornelas Ricart, Wagner Fontes, Celia Maria de Almeida Soares
Summary: Paracoccidioides brasiliensis reprograms its metabolism under hypoxia to reduce basal metabolism, increase energy and general metabolism, and modify glucose utilization for glycolysis or cell wall synthesis. Changes in plasma membrane/cell wall composition and activation of detoxification mechanisms are also observed. Proteomic analysis revealed up-regulation of biochemical pathways related to acetyl-CoA, pyruvate, and ergosterol synthesis.Metabolic adaptation processes differ between Paracoccidioides species, with distinct proteome profiles in response to hypoxia.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Eduardo Bagagli, Daniel Ricardo Matute, Hans Garcia Garces, Bernardo Guerra Tenorio, Adalberto Garcia Garces, Lucas Gomes de Brito Alves, Danielle Hamae Yamauchi, Marluce Francisca Hrycyk, Bridget Marie Barker, Marcus de Melo Teixeira
Summary: This study found coexistence of P. brasiliensis and P. lutzii in armadillos in Brazil, as well as evidence of two isolates resulting from admixture within P. brasiliensis sensu stricto. The results suggest gene exchange within P. brasiliensis sensu stricto populations and highlight the importance of population structure and admixture for pathogenic fungi.
Article
Immunology
Kaila Petronila Medina-Alarcon, Iara Pengo Tobias da Silva, Giovana Garcia Ferin, Marcelo A. Pereira-da-Silva, Caroline Maria Marcos, Mariana Bastos dos Santos, Luis Octavio Regasini, Marlus Chorilli, Maria Jose S. Mendes-Giannini, Fernando Rogerio Pavan, Ana Marisa Fusco-Almeida
Summary: Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis is a public health problem in Latin America, with the lack of adequate diagnosis leading to severe outcomes. The study showed the formation of mixed biofilms of these microorganisms and the potential effectiveness of a new treatment method.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Rafael de Souza Silva, Wilson Dias Segura, Reinaldo Souza Oliveira, Patricia Xander, Wagner Luiz Batista
Summary: Paracoccidioidomycosis (PCM) is a prevalent fungal infection in Latin America, caused by fungi from the Paracoccidioides genus. This study characterized the modulation of acid proteases in Paracoccidioides brasiliensis and found that these proteases are regulated by environmental conditions and fungal thermo-dimorphism. The findings provide new possibilities for studying the role of aspartyl proteases in the host-pathogen relationship and P. brasiliensis biology.
Article
Immunology
Relber Aguiar Goncales, Rafael Ricci-Azevedo, Vanessa C. S. Vieira, Fabricio F. Fernandes, Sandra M. de O. Thomaz, Agostinho Carvalho, Patricia E. Vendruscolo, Cristina Cunha, Maria Cristina Roque-Barreira, Fernando Rodrigues
Summary: The study demonstrated that overexpression of PCN in Paracoccidioides brasiliensis enhances cell separation and chitin processing, leading to increased virulence and exacerbation of the disease. This suggests that PCN modulates cell wall architecture through its chitinase activity.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Leandro B. R. Silva, Cleison L. Taira, Levi G. Cleare, Michele Martins, Magno Junqueira, Joshua D. Nosanchuk, Carlos P. Taborda
Summary: By using immunoproteomics, 18 novel immunogenic peptides derived from Paracoccidioides lutzii were identified, with 8 of them showing promising potential for future therapeutic approaches against PCM.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Ana Marina Barroso de Figueiredo, Dayane Moraes, Alexandre Melo Bailao, Olivia Basso Rocha, Lana Ohara Souza Silva, Fatima Ribeiro-Dias, Celia Maria de Almeida Soares
Summary: Proteomic analysis revealed the impact of P. brasiliensis yeast cells on the macrophage proteome, indicating metabolic changes, altered energy production pathways, epigenetic modifications, gene transcription, and immune system activity. These findings contribute to understanding the host response to P. brasiliensis and identifying potential therapeutic targets for PCM.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
E. K. Grasset, A. Chorny, S. Casas-Recasens, C. Gutzeit, G. Bongers, I Thomsen, L. Chen, Z. He, D. B. Matthews, M. A. Oropallo, P. Veeramreddy, M. Uzzan, A. Mortha, J. Carrillo, B. S. Reis, M. Ramanujam, J. Sintes, G. Magri, P. J. Maglione, C. Cunningham-Rundles, R. J. Bram, J. Faith, S. Mehandru, O. Pabst, A. Cerutti
SCIENCE IMMUNOLOGY
(2020)
Article
Environmental Sciences
Hellem Cristina Silva Carneiro, Rafael Wesley Bastos, Noelly Queiroz Ribeiro, Ludmila Gouveia-Eufrasio, Marliete Carvalho Costa, Thais Furtado Ferreira Magalhaes, Lorena Vivien Neves Oliveira, Tatiane Alves Paixao, Luna Sobrino Joffe, Marcio L. Rodrigues, Glauber Ribeiro de Sousa Araujo, Susana Frases, Jeronimo Conceicao Ruiz, Paula Marinho, Jonatas Santos Abrahao, Maria Aparecida Resende-Stoianoff, Dee Carter, Daniel Assis Santos
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Multidisciplinary Sciences
Zuri A. Sullivan, William Khoury-Hanold, Jaechul Lim, Chris Smillie, Moshe Biton, Bernardo S. Reis, Rachel K. Zwick, Scott D. Pope, Kavita Israni-Winger, Roham Parsa, Naomi H. Philip, Saleh Rashed, Noah Palm, Andrew Wang, Daniel Mucida, Aviv Regev, Ruslan Medzhitov
Summary: Research has shown that carbohydrate intake plays a vital role in regulating intestinal epithelial gene expression and the enzymes and transport proteins required for carbohydrate digestion and absorption; gamma delta T cells regulate this process by suppressing interleukin-22 production by type 3 innate lymphoid cells; nutrient availability affects the tissue localization and transcriptome of gamma delta T cells, as well as the cellular remodeling of the epithelial compartment.
Article
Immunology
Jayati Basu, Bernardo S. Reis, Suraj Peri, Jikun Zha, Xiang Hua, Lu Ge, Kyle Ferchen, Emmanuelle Nicolas, Philip Czyzewicz, Kathy Q. Cai, Yinfei Tan, Juan I. Fuxman Bass, Albertha J. M. Walhout, H. Leighton Grimes, Sergei Grivennikov, Daniel Mucida, Dietmar J. Kappes
Summary: The transcription factor ThPOK plays a critical role in the homeostasis and differentiation of mature helper T cells, controlling the expression of Sil(ThPOK) in CD4 T cells through an autoregulatory loop. Disruption of this loop leads to changes in chromatin accessibility and the derepression of colonic T-reg cell gene expression, promoting the differentiation of naive CD4 T cells into specific T-reg cells and CD4(+) IELs, providing protection from colitis in the gut. This autoregulatory loop represents a key mechanism for the physiological control of ThPOK expression and T cell differentiation in the gut with potential therapeutic relevance.
Article
Engineering, Electrical & Electronic
Thiago Alonso Stephan Lacerda de Sousa, Viviane Cristina Fernandes dos Santos, Nathalie Bonatti Franco Almeida, Fabricio Aparecido dos Santos, Thais Gomes da Silva, Eduardo Nery Duarte de Araujo, Antero Silva Ribeiro de Andrade, Flavio Plentz
Summary: Surface modifications with organic molecules are a versatile tool for enabling graphene applications in biotechnology. Interfacing graphene with ligands such as antibodies and aptamers is a prevalent methodology for creating bios-elective graphene surfaces. By using two novel routes for surface modifications of graphene devices with Staphylococcus aureus aptamers, researchers have successfully achieved selective graphene surfaces which provide robust alternatives for constructing tailored graphene-based biosensors.
IEEE SENSORS JOURNAL
(2021)
Article
Immunology
Hernandez Moura Silva, Jamil Zola Kitoko, Camila Pereira Queiroz, Lina Kroehling, Fanny Matheis, Katharine Lu Yang, Bernardo S. Reis, Christine Ren-Fielding, Dan R. Littman, Marcelo Torres Bozza, Daniel Mucida, Juan J. Lafaille
Summary: Perivascular macrophages play a role in tissue development and disease processes, although their key features are not yet clear. Lack of the transcription factor c-MAF in perivascular macrophages leads to different effects in various organs and has significant impacts on metabolic parameters.
SCIENCE IMMUNOLOGY
(2021)
Article
Immunology
Roham Parsa, Mariya London, Tiago Bruno Rezende de Castro, Bernardo Reis, Julian Buissant des Amorie, Jason G. Smith, Daniel Mucida
Summary: The intestinal epithelium is the largest surface in the body that is exposed to viruses and houses a large population of intraepithelial T lymphocytes. This study found that newly recruited CD4(+) T cells accumulate in the intestine after infection with certain viruses, and they provide protection against enteric adenovirus. These findings reveal a potential protective role of intraepithelial Ly6A(+)CCR9(+)CD4(+) T cells against enteric adenovirus.
Article
Multidisciplinary Sciences
Bernardo S. Reis, Patrick W. Darcy, Iasha Z. Khan, Christine S. Moon, Adam E. Kornberg, Vanessa S. Schneider, Yelina Alvarez, Olawale Eleso, Caixia Zhu, Marina Schernthanner, Ainsley Lockhart, Aubrey Reed, Juliana Bortolatto, Tiago B. R. Castro, Angelina M. Bilate, Sergei Grivennikov, Arnold S. Han, Daniel Mucida
Summary: Gamma delta T cells in colorectal cancer (CRC) exhibit distinct phenotypes and functions at different stages of CRC development. In premalignant or non-tumor colons, gamma delta T cells show cytotoxic markers, while tumor-infiltrating gamma delta T cells express a pro-tumorigenic profile.
Article
Multidisciplinary Sciences
Djenet Bousbaine, Laura Fisch, Mariya London, Preksha Bhagchandani, Tiago B. Rezende de Castro, Mark Mimee, Scott Olesen, Bernardo S. Reis, David VanInsberghe, Juliana Bortolatto, Mathilde Poyet, Ross W. Cheloha, John Sidney, Jingjing Ling, Aaron Gupta, Timothy K. Lu, Alessandro Sette, Eric J. Alm, James J. Moon, Gabriel D. Victora, Daniel Mucida, Hidde L. Ploegh, Angelina M. Bilate
Summary: The microbiome plays a role in the development and maturation of the immune system. CD4(+) T cells in the intestine differentiate into different subtypes in response to commensal bacteria. The development of CD4IELs in the small intestine depends on the microbiota. Beta-hexosaminidase, a conserved enzyme in commensal bacteria, drives the differentiation of CD4IELs. In a mouse model of colitis, beta-hexosaminidase-specific lymphocytes protect against intestinal inflammation.
Article
Biochemistry & Molecular Biology
Mariane Izabella Abreu de Melo, Pricila da Silva Cunha, Ieda Mendes Ferreira, Antero Silva Ribeiro de Andrade
Summary: This study successfully selected DNA aptamers with high affinity and specificity for Staphylococcus aureus, which have the potential to be used in the diagnosis of S. aureus and other bacterial infections.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Yu Matsuzawa-Ishimoto, Xiaomin Yao, Akiko Koide, Beatrix M. Ueberheide, Jordan E. Axelrad, Bernardo S. Reis, Roham Parsa, Jessica A. Neil, Joseph C. Devlin, Eugene Rudensky, M. Zahidunnabi Dewan, Michael Cammer, Richard S. Blumberg, Yi Ding, Kelly Ruggles, Daniel Mucida, Shohei Koide, Ken Cadwell
Summary: Loss of Paneth cells is associated with Crohn's disease. γδ IELs promote the viability of Paneth cells by secreting API5.
Article
Nanoscience & Nanotechnology
Nathalie B. F. Almeida, Thiago A. S. L. Sousa, Viviane C. F. Santos, Camila M. S. Lacerda, Thais G. Silva, Rafaella F. Q. Grenfell, Flavio Plentz, Antero S. R. Andrade
Summary: In this study, an aptamer (ZIKV60) with high affinity and specificity to Zika virus NS1 protein was selected through CE-SELEX. The ZIKV60 aptamer showed a strong binding affinity to ZIKV NS1, and exhibited high specificity compared to NS1 from DENV and YFV. The functionalized graphene field-effect transistor devices with ZIKV60 demonstrated the potential for differential diagnosis of Zika virus.
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
(2022)
Article
Virology
Camila Maria de Sousa Lacerda, Nathalie Bonatti Franco Almeida, Viviane Cristina Fernandes dos Santos, Flavio Plentz, Antero Silva Ribeiro de Andrade
Summary: This study aimed to obtain DNA aptamers specific for the 3ABC protein of FMDV for their application in FMD diagnosis. An aptamer named FMDV1 was selected using a variation of the SELEX technique called Capillary Electrophoresis SELEX (CE-SELEX). The FMDV1 aptamer showed high binding affinity to the 3ABC protein with a Kd value of 22.69 +/- 1.79 nM, and its binding to 3ABC was significantly higher compared to the control BSA protein, demonstrating its specificity.
Article
Immunology
Sandra Nakandakari-Higa, Roham Parsa, Bernardo S. Reis, Renan V. H. de Carvalho, Luka Mesin, Hans-Heinrich Hoffmann, Juliana Bortolatto, Hiromi Muramatsu, Paulo. J. C. Lin, Angelina M. Bilate, Charles M. Rice, Norbert Pardi, Daniel Mucida, Gabriel D. Victora, Maria Cecilia C. Canesso
Summary: Efficient mouse models are crucial for studying SARS-CoV-2 infection and evaluating vaccines and treatments. Researchers have developed a mouse model that can be infected by multiple strains of SARS-CoV-2 through minimal genetic modifications. The infected mice exhibit similar clinical manifestations to COVID-19 patients, making the model valuable for studying immune responses to sequential infections.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Lili Chen, Zhengxiang He, Bernardo S. Reis, Jesse D. Gelles, Jerry Edward Chipuk, Adrian T. Ting, Julie A. Spicer, Joseph A. Trapani, Glaucia C. Furtado, Sergio A. Lira
Summary: Using single-cell transcriptomic analysis, researchers identified multiple subsets of CD4(+) T cells in the intestines of colitic mice, including a subset that produces interferon (IFN)-gamma. In vivo experiments showed that challenge with the food colorant Red 40 activated CD4(+) T cells and caused intestinal epithelial cell apoptosis, which was attenuated by depletion of CD4(+) cells and blocking IFN-gamma. Experiments conducted ex vivo demonstrated that intestinal CD4(+) T cells from colitic mice directly promoted apoptosis of intestinal epithelial cells and enteroids.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
