Article
Plant Sciences
J. R. Standish, E. Gongora-Castillo, M. J. Bowman, K. L. Childs, M. Tian, L. M. Quesada-Ocampo
Summary: In this study, a bioinformatics pipeline was developed to predict specific diagnostic markers for Peronospora belbahrii, the causative agent of basil downy mildew. These markers were validated and used to develop a highly sensitive qPCR assay for detecting P. belbahrii in leaf tissue and seed samples. The assay was able to detect low concentrations of P. belbahrii genomic DNA and a small number of sporangia. This research is valuable for diagnostics and identifying contaminated seed lots to mitigate the effects of basil downy mildew epidemics.
Article
Plant Sciences
Kazuki Fujiwara, Hiroyoshi Inoue, Ryoichi Sonoda, Yutaka Iwamoto, Motoaki Kusaba, Nobuya Tashiro, Atsushi Miyasaka
Summary: The study shows that P. destructor can be detected in both asymptomatic plants and soil, indicating a potential strategy to optimize disease management for onion downy mildew. The real-time PCR assay using novel primer sets provides a useful tool for identifying and quantifying inoculum sources.
Article
Immunology
Yu-Ting Chiu, Ni Tien, Hsiao-Chuan Lin, Hsiu-Mei Wei, Huan-Cheng Lai, Jiun-An Chen, Yan-Yi Low, Hsiu-Hsien Lin, Yu-Lung Hsu, Kao-Pin Hwang
Summary: This study analyzed the epidemiology and clinical presentations of upper and lower respiratory tract infections. The positive rate of tested respiratory samples among adults and children was 21.48% and 48.31%, respectively. Human rhinovirus/Enterovirus (HRV/EV) was commonly found in all age groups, with a higher prevalence in upper respiratory tract infections, while Mycoplasma pneumoniae was the most common pathogen in lower respiratory tract infections.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Immunology
Ying Luo, Ying Xue, Yimin Cai, Qun Lin, Guoxing Tang, Huijuan Song, Wei Liu, Liyan Mao, Xu Yuan, Yu Zhou, Weiyong Liu, Shiji Wu, Ziyong Sun, Feng Wang
Summary: Inadequate tuberculosis diagnostics, especially in discriminating between active TB and latent TB infection, are major obstacles in disease burden reduction. The study investigated the role of lymphocyte non-specific function detection for TB diagnosis. Results show that CD4(+) T cell non-specific function has the potential for differentiating T-SPOT results, and the three-index diagnostic model is a potent tool for identifying different events in TB infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Zoology
David A. Strand, Tomas Jinnerot, Anna Aspan, Satu Viljamaa-Dirks, Sirpa Heinikainen, Elin Rolen, Trude Vralstad
Summary: The parasitic oomycete Aphanomyces astaci is the causative agent of crayfish plague, a devastating disease for European freshwater crayfish. The well established detection method may lead to false-positive results, highlighting the need for improvement to avoid amplification of other Aphanomyces species.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Agronomy
Miting Wan, Liyun Yang, Shizi Zhang, Jing Gao, Lingyan Jiang, Lijuan Luo
Summary: In this study, a real-time PCR assay based on ACT was developed for the detection and quantification of Colletotrichum gloeosporioides s.l. The assay displayed high specificity, allowing early detection at the symptomless phase of infection, and showed good correlation with microscopic observation and later disease symptoms. The method provides a rapid and effective detection tool for studying plant-Colletotrichum interactions, disease prediction, and control.
Article
Chemistry, Analytical
Liben Chen, Kai Wen, Fan-En Chen, Alexander Y. Trick, Hebin Liu, Shibei Shao, Wenbo Yu, Kuangwen Hsieh, Zhanhui Wang, Jianzhong Shen, Tza-Huei Wang
Summary: African swine fever (ASF), with a nearly 100% mortality rate, has devastated pork industries around the world. In the absence of a vaccine, a portable device has been developed for rapid ASF detection, exhibiting high diagnostic accuracy in farm and slaughter facility settings.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Lu Wang, Pan Wu, Yingying Su, Yi Wei, Xi Guo, Lan Yang, Min Wang, Bin Liu
Summary: This study identified novel marker genes for the genus Cronobacter, as well as specific marker genes for three species within the genus. Duplex and multiplex PCR methods based on these markers were established and validated for accurate detection of the strains. The developed methods can be used as valuable tools for identifying these strains in various samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Xiaolan Lin, Jiaxin Ma, Zanhu Zhou, Baoyi Qiao, Yixin Li, Wei Zheng, Yun Tian
Summary: Through simulated experiments using diesel oil as a pollutant, it was found that pathogenic bacteria in the ocean are significantly enriched under oil contamination, with high toxicity and drug resistance. Infection experiments demonstrated the synergistic effect of toxic oil pollutants and pathogens on infected fish. The study also revealed the widespread presence of pathogenic bacteria with oil degradation potential in oil-contaminated marine environments, indicating extensive pathogen reservoir risks in these sites.
Article
Plant Sciences
Hanna Mestdagh, Kris Van Poucke, Annelies Haegeman, Tinne Dockx, Isabel Vandevelde, Ellen Dendauw, An Decombel, Monica Hoefte, Kurt Heungens
Summary: This study developed real-time PCR assays for the detection of Fusarium oxysporum f.sp.lactucae (Fol) races 1 and 4, prevalent in Europe. The assays were specific and sensitive, allowing for the detection of low amounts of genomic DNA. Sample preparation methods were also developed for different sample types, enabling the detection of Fol in plant tissue, soil, and surfaces. The assays were applied to commercial lettuce farms in Belgium and confirmed the prevalence of Fol race 4. The developed methods are useful for epidemiological research and evaluating control measures.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Rui Wang, Ying Liu, Shuaiwei Chen, Linlin Bai, Kaiming Guo, Yanan Pang, Feng Qian, Yongfang Li, Li Ding, Yongming Wang
Summary: Bloodstream infection is a global health problem with high mortality. This study proposes a novel strategy called utPCR that combines the sensitivity of digital PCR with a shortened reaction time, enabling single-molecule detection. The utPCR shows promising applications in the next generation of molecular diagnosis.
Article
Immunology
Li Yi, Manyu Jin, Mengxia Gao, Haikun Wang, Qingying Fan, Daniel Grenier, Liyun Sun, Shaohui Wang, Yang Wang
Summary: Respiratory infections greatly impact the swine industry globally, with co-infections of Streptococcus suis and Actinobacillus pleuropneumoniae often forming biofilms. However, the methods for detecting these bacteria in co-infections and biofilms are immature. This study successfully established a TaqMan real-time PCR method for simultaneous detection of S. suis and A. pleuropneumoniae, which may aid in disease detection, prevention, and control.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Liangsheng Xu, Yibo Wang, Shan Zhu, Jianyu Li, Yali Chang, Lili Huang
Summary: Valsa mali, the causal agent of apple Valsa canker, can be effectively diagnosed using the field-based LAMP assay, which is rapid, sensitive, and reliable. The LAMP method targets a specific DNA region and can detect the disease with high sensitivity, making it a practical tool for disease management in apple orchards.
Article
Microbiology
Bangchuan Hu, Yue Tao, Ziqiang Shao, Yang Zheng, Run Zhang, Xuejing Yang, Jingquan Liu, Xi Li, Renhua Sun
Summary: This study compared the diagnostic utility of ddPCR and mNGS for critically ill patients with suspected BSIs. It found that ddPCR was faster and more sensitive for common isolated pathogens and AMR genes, while mNGS detected a broader range of pathogens, including viruses. Based on their respective strengths and limitations, ddPCR is more suitable for rapid detection of common isolated pathogens and AMR genes, while mNGS is more appropriate for diagnosing BSIs when causative pathogens cannot be identified by traditional methods.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Linqing Zhao, Xiaoyan Dong, Ling Cao, Yongmei Jiang, Hong Zhang, Ran Mo, Hanmin Liu
Summary: This study evaluated the performance of Acaruiter Respiratory Panel (fluorescent PCR) in detecting respiratory tract infection pathogens in children. The results showed that it has good consistency, high accuracy, and high positive and negative agreement percentages.
MICROBIOLOGY SPECTRUM
(2023)
Article
Soil Science
Doreen Babin, Annette Deubel, Samuel Jacquiod, Soren J. Sorensen, Joerg Geistlinger, Rita Grosch, Kornelia Smalla
SOIL BIOLOGY & BIOCHEMISTRY
(2019)
Article
Microbiology
Azhar A. Zarkani, Jasper Schierstaedt, Marlies Becker, Johannes Krumwiede, Maja Grimm, Rita Grosch, Sven Jechalke, Adam Schikora
FEMS MICROBIOLOGY ECOLOGY
(2019)
Article
Microbiology
Jasper Schierstaedt, Sven Jechalke, Joseph Nesme, Klaus Neuhaus, Soren J. Sorensen, Rita Grosch, Kornelia Smalla, Adam Schikora
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Agronomy
R. Djalali Farahani-Kofoet, R. Duensing, F. Braendle, R. Grosch
Article
Multidisciplinary Sciences
Sneha Gulati, Max-Bernhard Ballhausen, Purva Kulkarni, Rita Grosch, Paolina Garbeva
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Rita Zrenner, Franziska Genzel, Bart Verwaaijen, Daniel Wibberg, Rita Grosch
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Saskia Windisch, Loreen Sommermann, Doreen Babin, Soumitra Paul Chowdhury, Rita Grosch, Narges Moradtalab, Frank Walker, Birgit Hoeglinger, Abbas El-Hasan, Wolfgang Armbruster, Joseph Nesme, Soren Johannes Sorensen, Ingo Schellenberg, Joerg Geistlinger, Kornelia Smalla, Michael Rothballer, Uwe Ludewig, Guenter Neumann
Summary: Fertilization management has specific effects on rhizodeposition, rhizosphere microbiota, and plant performance. The study demonstrates a complex network of interactions among root exudates, site-specific factors and rhizosphere microbiota that modulate the impact of fertilization management on plant health and performance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Tarek R. Elsayed, Rita Grosch, Kornelia Smalla
Summary: This study found that bacteria with antagonistic activity towards Ralstonia solanacearum were enriched in the rhizosphere and endophytic compartments of potato plants grown in different soil types, indicating the importance of plant sphere in shaping the proportion and diversity of antagonists.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Plant Sciences
Saskia Windisch, Anja Walter, Narges Moradtalab, Frank Walker, Birgit Hoeglinger, Abbas El-Hasan, Uwe Ludewig, Guenter Neumann, Rita Grosch
Summary: The study identified benzoic acid and lettucenin A as defense compounds against Rhizoctonia solani and Olpidium virulentus in lettuce. Benzoic acid was released as root exudate, inhibiting R. solani growth and mitigating growth suppression and root damage in lettuce. Lettucenin A acted as a phytoalexin, accumulating locally in affected plant tissues upon infection.
Article
Microbiology
Daniel Wibberg, Franziska Genzel, Bart Verwaaijen, Jochen Blom, Oliver Rupp, Alexander Goesmann, Rita Zrenner, Rita Grosch, Alfred Puehler, Andreas Schlueter
Summary: The study compared the genome sequences of two less aggressive Rhizoctonia solani isolates with a highly aggressive isolate, revealing potential gene differences that may explain the varying levels of aggressiveness.
Article
Agronomy
Jan Graefe, Rita Grosch, Michael Bitterlich
Summary: The study focused on the efficiency of heat and mass exchange between leaves and their environment under low wind speed conditions. Through numerical simulations, a new model of natural convection relationships was derived for different inclinations and elliptical shapes of leaves, with a predicted heat flux error of only 4% for temperature differences of 1-12K.
AGRICULTURAL AND FOREST METEOROLOGY
(2022)
Article
Microbiology
Kenneth Dumack, Kai Feng, Sebastian Flues, Melanie Sapp, Susanne Schreiter, Rita Grosch, Laura E. Rose, Ye Deng, Kornelia Smalla, Michael Bonkowski
Summary: An experiment in the field examined the effects of soil type and plant species on the assembly of soil protist communities. The results confirmed the presence of plant species-specific protist communities and identified mechanisms of self-organization.
Article
Engineering, Environmental
Nikola Major, Sven Jechalke, Joseph Nesme, Smiljana Goreta Ban, Marko Cerne, Soren J. Sorensen, Dean Ban, Rita Grosch, Adam Schikora, Jasper Schierstaedt
Summary: Municipal sewage sludge and other biosolids have high potential as organic fertilizers in agriculture, but their use is limited by the presence of heavy metal and organic compound pollutants. This study investigated the impact of stabilization method and wastewater treatment plant size on microbial community structure and gene abundance, finding antibiotic resistance genes and mobile genetic elements in all samples. The presence of certain heavy metals correlated with the presence of antibiotic resistance genes. It is important to assess pollutants and long-term effects before considering the use of sewage sludge as fertilizer.
Article
Plant Sciences
Burkhardt Flemer, Sneha Gulati, Alessandro Bergna, Manuela Raendler, Tomislav Cernava, Katja Witzel, Gabriele Berg, Rita Grosch
Summary: This study aimed to assess the impact of abiotic and biotic stress factors on tomato plant growth and root bacterial composition. The results showed that tomato growth and photosynthetic activity were reduced under all stress conditions. The microbiota structure and function changed, and stressor-specific enrichments of beneficial bacteria in the roots were discovered.
PHYTOBIOMES JOURNAL
(2022)
Review
Microbiology
Jasper Schierstaedt, Rita Grosch, Adam Schikora
FEMS MICROBIOLOGY LETTERS
(2019)